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Spectrophotometry Determination of Stimulant Drugs in Urine by Color Reaction with Tetrabromophenolphthalein Ethyl Ester

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Abstract

A new procedure for sensitive and selective determination of stimulant drugs in urine Is described. The method consists of extracting the organic base in alkaline media and performing the color reaction with tetrabromophenolphthalein ethyl ester molecule. Colored products have maximum absorption at 570 nm for methampheta-mine, 555 nm for ephedrine and 550 nm for methylephedrine, respectively. The optimal conditions — pH on extraction of organic bases, shaking time, concentration of dye, percentage extraction of the bases and the linear ranges of calibration curves are investigated for the determinations of methamphetamine, ephedrine and methylephedrine. The extracting solvent used is 1,2-dichloroethane. This new method allows the sensitive and selective determination of methamphetamine, ephedrine and/or methylephedrine in urine.

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Authors and Affiliations

  1. Department of Chemistry, Asahi University(Gifu College of Dentistry), Hozumi, Gifu, 501-02, Japan

    Tadao Sakai & Noriko Ohno

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  1. Tadao Sakai
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  2. Noriko Ohno
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Sakai, T., Ohno, N. Spectrophotometry Determination of Stimulant Drugs in Urine by Color Reaction with Tetrabromophenolphthalein Ethyl Ester. ANAL. SCI. 2, 275–279 (1986). https://doi.org/10.2116/analsci.2.275

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  • DOI: https://doi.org/10.2116/analsci.2.275

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