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Fast Separation and Quantification of C60 and C70 Fullerenes Using Thermostated Micro Thin-layer Chromatography

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Abstract

Thermostated micro planar chromatography was applied for systematic separation studies of C60 and C70 fullerenes using n-alkanes as mobile phases on TLC and HPTLC plates coated with polyamide, silica gel, aluminum oxide as well as two types of octadecylsilica (C18) sorbents. Retention data were collected at constant temperature at 20°C (±0.05°C) using an unsaturated chamber mode with an eluent, such as n-pentane, n-hexane and n-heptane. The separation results under both saturated and unsaturated chamber modes for selected mobile/stationary phases were also examined, and several parameters, including separation factor (α) and resolution (RS), were compared with data obtained with high¬performance liquid chromatography conditions. Interestingly, C60/C70 fullerenes separation performed on HPTLC plates with a developing distance of 45 mm was better for those observed on a 25 cm length analytical HPLC column under similar conditions to that on carbon coverage of the stationary phase, n-hexane as the mobile phase and separation temperature (RS = 1.84 and 1.68 for HPTLC, and HPLC, respectively). Moreover the advantage of the planar chromatographic separation of fullerenes studied is a short elution time of less than 6 min. Furthermore, the reported separation protocol shows a capability for the evaluation of fullerenes quantity in commercial samples.

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References

  1. D. M. Cox, S. Behal, M. Disko, S. M. Gorun, M. Greaney, C. S. Hsu, E. B. Kollin, J. Millar, J. Robbins, W. Robbins, R. D. Sherwood, and P. Tindall, J. Am. Chem. Soc., 1991, 113, 2940.

    Article  CAS  Google Scholar 

  2. J. C. Fetzer and E. J. Gallegos, Polycyclic Aromat. Compd., 1992, 2, 245.

    Article  CAS  Google Scholar 

  3. K. Jinno, “Separation of Fullerenes by Liquid Chromatography”, ed. RSC Chromatography Monographs, 1999, The Royal Society of Chemistry, Cambridge, UK.

    Google Scholar 

  4. Y. Saito, H. Ohta, and K. Jinno, Anal. Chem., 2004, 75 (15), 266A.

  5. H. Ohta, Y. Saito, N. Nagae, J. J. Pesek, M. T. Matyska, and K. Jinno, J. Chromatogr., A, 2000, 883, 55.

    Article  CAS  Google Scholar 

  6. J. P. Hare, H. W. Kroto, and R. Taylor, Chem. Phys. Lett., 1991, 177, 394.

    Article  CAS  Google Scholar 

  7. H. Ajie, M. M. Alvarez, S. J. Anz, R. D. Beck, F. Diederich, K. Fostiropoulos, D. R. Huffman, W. Kratschmer, Y. Rubin, K. E. Schriver, D. Sensharma, and R. L. Whetten, J. Phys. Chem., 1990, 94, 8630.

    Article  CAS  Google Scholar 

  8. C. J. Welch and W. H. Pirkle, J. Chromatogr., 1992, 609, 89.

    Article  CAS  Google Scholar 

  9. A. Gugel and K. Mullen J. Chromatogr., 1993, 628, 23.

    Article  Google Scholar 

  10. H. Ohta, K. Jinno, Y. Saito, J. C. Fetzer, W. R. Biggs, J. J. Pesek, M. T. Matyska, and Y. L. Chen, Chromatographia, 1996, 42, 56.

    Article  CAS  Google Scholar 

  11. P. K. Zarzycki, H. Ohta, Y. Saito, and J. Jinno, Chromatographia, 2006, 64, 79.

    Article  CAS  Google Scholar 

  12. R. Cody, J. Laramee, and H. D. Durst, Anal. Chem., 2005, 77, 2297.

    Article  CAS  Google Scholar 

  13. G. Morlock and W. Schwack, Anal. Bioanal. Chem., 2006, 385, 586.

    Article  CAS  Google Scholar 

  14. E. Soczewinski, “A. Century of Separation Science: Quantitative Retention-Eluent Composition Relationships in Partition and Adsorption Chromatography”, ed. H. J. Issaq, 2002, Chap. 11, Marcel Dekker, Inc., New York, 179.

  15. P. K. Zarzycki, K. M. Kulhanek, R. Smith, M. A. Bartoszuk, and H. Lamparczyk, LCGC North Am., 2005, 23, 286.

    Google Scholar 

  16. F. Djojo and A. Hirsch, Chem.—Eur. J., 1998, 4, 344.

    Article  CAS  Google Scholar 

  17. K. Lee, Y. J. Choi, Y.-J. Cho, C. Y. Lee, H. Song, C. H. Lee, Y. S. Lee, and J. T. Park, J. Am. Chem. Soc., 2004, 126, 9837.

    Article  CAS  Google Scholar 

  18. L. S. Litvinova, A. V. Gribanov, M. V. Mokeev, and V. N. Zgonnik, Russ. J. Appl. Chem., 2004, 77(3), 438.

    Article  CAS  Google Scholar 

  19. L. S. Litvinova, J. Planar Chromatogr., 2006, 19, 171.

    Article  CAS  Google Scholar 

  20. L. S. Litvinova and V. N. Zgonnik, J. Planar Chromatogr., 1997, 10, 38.

    CAS  Google Scholar 

  21. L. S. Litvinova and V. N. Zgonnik, J. AOAC Int., 1999, 82 (2), 227.

    Article  Google Scholar 

  22. L. S. Litvinova, V. G. Ivanov, and V. N. Zgonnik, J. Planar Chromatogr., 2001, 14, 343.

    CAS  Google Scholar 

  23. P. E. Wall, “Thin-layer Chromatography. A Modern Practical Approach”, 2006, Springer-Verlag, RSC, Cambridge.

    Google Scholar 

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Authors and Affiliations

  1. Section of Toxicology and Bioanalytics, Department of Environmental Biology, Koszalin University of Technology, Śniadeckich 2, 75-453, Koszalin, Poland

    Paweł K. Zarzycki & Filip B. Harasimiuk

  2. Toyohashi University of Technology, School of Materials Science, Toyohashi, 441-8580, Japan

    Hatsuichi Ohta & Kiyokatsu Jinno

  3. School of Materials Science at Toyohashi University of Technology, Japan, as the Senior Visiting Scientist within Section of Toxicology and Bioanalytics at Koszalin University of Technology, Poland

    Hatsuichi Ohta

Authors
  1. Paweł K. Zarzycki
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  2. Hatsuichi Ohta
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  3. Filip B. Harasimiuk
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  4. Kiyokatsu Jinno
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Correspondence to Paweł K. Zarzycki.

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Zarzycki, P.K., Ohta, H., Harasimiuk, F.B. et al. Fast Separation and Quantification of C60 and C70 Fullerenes Using Thermostated Micro Thin-layer Chromatography. ANAL. SCI. 23, 1391–1396 (2007). https://doi.org/10.2116/analsci.23.1391

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  • DOI: https://doi.org/10.2116/analsci.23.1391

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