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Determination of Short-chain Fatty Acids in Rat and Human Feces by High-Performance Liquid Chromatography with Electrochemical Detection

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Abstract

A simple method for determining short-chain fatty acids (SCFAs) in rat and human feces was developed using high-performance liquid chromatography with electrochemical detection (HPLC-ECD). A two-channel HPLC-ECD system was fabricated using an ion exclusion column and an electrochemical detector with a glassy carbon working electrode. Aqueous solutions of 0.1 mM HClO4 and of ethanol containing 2-methyl-1,4-naphthoquinone served as a mobile phase and a quinone solution, respectively. Peak areas for lactic, acetic, propionic, butyric, isovaleric, and valeric acids at a detection potential of −0.9 V vs. an Ag/AgCl electrode showed a linear relationship with the acid amount in the range 0.1 to 40 nmol. Standard acids at 4 nmol were determined ten times with relative standard deviations (RSD) of less than 2.0%. The analytical results of healthy human feces were measured within 35 min. RSD (n = 5) in all SCFAs were less than 2.7%, and recoveries of SCFAs were more than 92%. The present method was characterized by reproducibility with the simple and rapid procedure without derivatization of analytes, and it has the potential for clinical and biomedical applications.

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Authors and Affiliations

  1. Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, 192-0392, Hachioji, Tokyo, Japan

    Akira Kotani, Yuji Miyaguchi, Mototaka Kohama, Takafumi Ohtsuka & Fumiyo Kusu

  2. Department of Gastroenterology, Hachioji Medical Center of Tokyo Medical University, 1163 Tatemachi, 193-0998, Hachioji, Tokyo, Japan

    Taisei Shiratori

Authors
  1. Akira Kotani
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  2. Yuji Miyaguchi
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  3. Mototaka Kohama
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  4. Takafumi Ohtsuka
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  5. Taisei Shiratori
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  6. Fumiyo Kusu
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Correspondence to Akira Kotani.

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Kotani, A., Miyaguchi, Y., Kohama, M. et al. Determination of Short-chain Fatty Acids in Rat and Human Feces by High-Performance Liquid Chromatography with Electrochemical Detection. ANAL. SCI. 25, 1007–1011 (2009). https://doi.org/10.2116/analsci.25.1007

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  • DOI: https://doi.org/10.2116/analsci.25.1007

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