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Direct Drug Metabolism Monitoring in a Live Single Hepatic Cell by Video Mass Spectrometry

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Abstract

The metabolism of anti-breast cancer drug, tamoxifen, in a single human hepatocellular carcinoma cell, HepG2, was directly monitored by a video-mass spectroscope. The cytoplasm, a vacuole or nucleus of the cell was directly sucked by a nano-spray tip under a video-microscope, and then was introduced into a mass spectrometer. Unchanged drug molecules were found in cytoplasm and a vacuole, but the metabolites were only found in the cytoplasm. This direct detection of drug metabolites in a live single cell is useful for speedy drug metabolism monitoring.

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Authors and Affiliations

  1. Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, 565-0874, Suita, Osaka, Japan

    Sachiko Date & Tsutomu Masujima

  2. Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, 734-8551, Minami, Hiroshima, Japan

    Hajime Mizuno, Naohiro Tsuyama, Takanori Harada & Tsutomu Masujima

Authors
  1. Sachiko Date
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  2. Hajime Mizuno
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  3. Naohiro Tsuyama
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  4. Takanori Harada
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  5. Tsutomu Masujima
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Corresponding author

Correspondence to Tsutomu Masujima.

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Date, S., Mizuno, H., Tsuyama, N. et al. Direct Drug Metabolism Monitoring in a Live Single Hepatic Cell by Video Mass Spectrometry. ANAL. SCI. 28, 201–203 (2012). https://doi.org/10.2116/analsci.28.201

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  • DOI: https://doi.org/10.2116/analsci.28.201

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