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Expanding Possibilities of Rolling Circle Amplification as a Biosensing Platform

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Abstract

Rolling circle amplification (RCA) catalyzed by ϕ29 DNA polymerase offers a simple method for DNA amplification in the presence of a circular DNA template and its complimentary primer. RCA continuously produces long single-strand DNA using the strand displacement activity of polymerase during DNA synthesis. This property allows one to monitor the progress of a reaction by means of electrophoresis or fluorescence measurements, and has eventually allowed the application of RCA to signal increments in the sensing of a variety of molecular species. Originally, RCA was successfully applied for the detection of specific DNA, such as single nucleotide polymorphisms. In addition, the conjugation of an antibody with a primer achieves efficient signal enhancement in antigen detection, and mRNA can also be specifically detected. Since RCA is a carry-over contamination-resistant, cost-effective, and user-friendly method of DNA amplification, RCA could be a universal technology for biosensing in fields of medical- and food-related industries.

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Author notes

  1. Hirokazu Takahashi

    Present address: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, 739-8530, Higashi-hiroshima, Hiroshima, Japan

Authors and Affiliations

  1. National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kan-nondai, 305-8642, Tsukuba, Ibaraki, Japan

    Toshiro Kobori & Hirokazu Takahashi

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  1. Toshiro Kobori
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  2. Hirokazu Takahashi
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Correspondence to Toshiro Kobori.

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Kobori, T., Takahashi, H. Expanding Possibilities of Rolling Circle Amplification as a Biosensing Platform. ANAL. SCI. 30, 59–64 (2014). https://doi.org/10.2116/analsci.30.59

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