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A Rapid and Automated Device for Purifying Nucleic Acids

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Abstract

We have developed a rapid, automated nucleic acid purification device in a single cartridge containing silica-coated magnetic beads. We succeeded in extracting the matrix protein gene of influenza A virus from pharyngeal swab samples within 3 min. The device will be widely applicable to detect a specific gene from the various samples for clinical diagnosis and genetic research.

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Acknowledgments

We thank S. Koide, R. Kanai, T. Negishi, and T. Arai for technical assistance. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology (MEXT Japan).

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Authors and Affiliations

  1. Core Technology Development Center, Corporate Research and Development Div., Seiko Epson Corporation, 281 Fujimi, Fujimi-machi, Suwa, Nagano, 399-0293, Japan

    Masayuki Uehara, Masato Hanamura, Kiyohito Yamada, Akemi Yamaguchi, Toshiro Murayama, Yuji Saito & Kotaro Idegami

  2. Division of Medical Seeds Promotion, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan

    Masayuki Uehara & Takayuki Honda

  3. Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, 390-8621, Japan

    Takayuki Honda

Authors
  1. Masayuki Uehara
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  2. Masato Hanamura
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  3. Kiyohito Yamada
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  4. Akemi Yamaguchi
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  5. Toshiro Murayama
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  6. Yuji Saito
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  7. Kotaro Idegami
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  8. Takayuki Honda
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Corresponding author

Correspondence to Masayuki Uehara.

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Uehara, M., Hanamura, M., Yamada, K. et al. A Rapid and Automated Device for Purifying Nucleic Acids. ANAL. SCI. 32, 371–374 (2016). https://doi.org/10.2116/analsci.32.371

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  • DOI: https://doi.org/10.2116/analsci.32.371

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