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Separation and Determination of Alkaline-Earth Metal Ions as UV-Absorbing Chelates with EDTA by Capillary Electrophoresis. Determination of Calcium and Magnesium in Water and Serum Samples

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Abstract

Capillary electrophoresis of alkaline-earth metal ions was examined with a UV-absorbing chelating agent. The metal chelates separated in a capillary were measured by on-column UV-absorptive detection. When ethylenediamine-tetraacetic acid (EDTA) was used as an chelating agent in a carrier solution (pH 9.2), the order of the migration time (fm) of metal ions was as follows: Ba2+<Sr2+<Ca2+<Mg2* (Be2+ could not be detected). These four ions were separated within 16 min using a capillary (50 μm i.d.) of 75-cm effective length (Ld) at an applied voltage of 30 kV, and calcium and magnesium ions were separated within 4 min using a 25-cm capillary at an applied voltage of 20 kV. By using a 50-cm or 25-cm capillary, Ca2+ and Mg2+ in river, tap and underground water samples were determined; the detection limits of metal ions were about.10-5 M (1 M=moldm-3), and the relative standard deviations for the determination of around 10-4 M of the metal ions were less than 2.0%. The method was also applied to the determination of Ca2+ and Mg2+ in a standard serum sample.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Okayama University, Tsushimanaka, Okayama, 700, Japan

    Shoji Motomizu, Mitsuko Oshima & Shin-ya Matsuda

  2. Applied Biosystems Japan, Inc., 3-3-6, Minamisuna, Koto, Tokyo, 136, Japan

    Yoshimitsu Obata & Hiroshi Tanaka

Authors
  1. Shoji Motomizu
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  2. Mitsuko Oshima
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  3. Shin-ya Matsuda
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  4. Yoshimitsu Obata
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  5. Hiroshi Tanaka
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Motomizu, S., Oshima, M., Matsuda, Sy. et al. Separation and Determination of Alkaline-Earth Metal Ions as UV-Absorbing Chelates with EDTA by Capillary Electrophoresis. Determination of Calcium and Magnesium in Water and Serum Samples. ANAL. SCI. 8, 619–625 (1992). https://doi.org/10.2116/analsci.8.619

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  • DOI: https://doi.org/10.2116/analsci.8.619

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