The Japanese Journal of Physiology
Print ISSN : 0021-521X
Short Communications
A Semi-Quantitative PCR Method for the Detection of Low Levels of Apoptotic DNA Fragmentation in a Heart Failure Model
Craig S. McLachlanJian Lin YinCatherine DriussiPatricia R. JusufBrett HamblyMark A. McGuire
Author information
JOURNAL FREE ACCESS

2000 Volume 50 Issue 2 Pages 281-284

Details
Abstract

Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.

Content from these authors
© 2000 by The Physiological Society of Japan
Previous article Next article
feedback
Top