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Research Article

Silver-based nanoparticles induce apoptosis in human colon cancer cells mediated through p53

    Shakti Ranjan Satapathy

    Cancer Biology Division, KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa, 751024, India

    ,
    Purusottam Mohapatra

    Cancer Biology Division, KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa, 751024, India

    ,
    Ranjan Preet

    Cancer Biology Division, KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa, 751024, India

    ,
    Dipon Das

    Cancer Biology Division, KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa, 751024, India

    ,
    Biplab Sarkar

    Nanobiotechnology Division, KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa, 751024, India

    ,
    Tathagata Choudhuri

    Department of Infection Biology, Institute of Life Science, Nalco Square, Bhubaneswar, Orissa, 751021, India

    ,
    Michael D Wyatt

    Department of Pharmaceutical & Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC, USA.

    &
    Chanakya Nath Kundu

    * Author for correspondence

    Cancer Biology Division, KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa, 751024, India

    Published Online:https://doi.org/10.2217/nnm.12.176

    Aim: The authors have systematically investigated the anticancer potentiality of silver-based nanoparticles (AgNPs) and the mechanism underlying their biological activity in human colon cancer cells. Materials & methods: Starch-capped AgNPs were synthesized, characterized and their biological activity evaluated through multiple biochemical assays. Results: AgNPs decreased the growth and viability of HCT116 colon cancer cells. AgNP exposure increased apoptosis, as demonstrated by an increase in 4´,6-diamidino-2-phenylindole-stained apoptotic nuclei, BAX/BCL-XL ratio, cleaved poly(ADP-ribose) polymerase, p53, p21 and caspases 3, 8 and 9, and by a decrease in the levels of AKT and NF-κB. The cell population in the G1 phase decreased, and the S-phase population increased after AgNP treatment. AgNPs caused DNA damage and reduced the interaction between p53 and NF-κB. Interestingly, no significant alteration was noted in the levels of p21, BAX/BCL-XL and NF-κB after AgNP treatment in a p53-knockout HCT116 cell line. Conclusion: AgNPs are bona fide anticancer agents that act in a p53-dependent manner.

    Original submitted 16 March 2012; Revised submitted 25 August 2012; Published online 21 March 2013

    Papers of special note have been highlighted as: ▪ of interest

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