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Regenerative Medicine

Regeneration of dental pulp after pulpotomy by transplantation of CD31-/CD146- side population cells from a canine tooth

    Koichiro Iohara

    Department of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics & Gerontology, Obu, Aichi.

    ,
    Li Zheng

    Department of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics & Gerontology, Obu, Aichi.

    ,
    Masataka Ito

    Department of Developmental Anatomy & Regenerative Medicine, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan

    ,
    Ryo Ishizaka

    Department of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics & Gerontology, Obu, Aichi.

    Department of Pediatric Dentistry, School of Dentistry, Aichigakuin University, Nagoya, Aichi 464-8651, Japan

    ,
    Hiroshi Nakamura

    Department of Endodontology, School of Dentistry, Aichigakuin University, Nagoya, Aichi 464-8651, Japan

    ,
    Takeshi Into

    Department of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics & Gerontology, Obu, Aichi.

    ,
    Kenji Matsushita

    Department of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics & Gerontology, Obu, Aichi.

    &
    Misako Nakashima

    † Author for correspondence

    Department of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics & Gerontology, Obu, Aichi.

    Published Online:https://doi.org/10.2217/rme.09.5

    Aim: To achieve complete regeneration of dental pulp in vivo by stem/progenitor cells obtained from a fraction of side population (SP) cells from canine pulp. Materials & methods: A subfraction of SP cells, CD31-/CD146- SP cells, were isolated by flow cytometry from canine dental pulp. The efficiency of this subfraction of SP cells was evaluated in an experimental model of pulp injury in the dog. Results: The fractionated SP cells formed extensive networks of tube-like structures in vitro. Transplantation of the SP cells into an in vivo model of amputated pulp resulted in complete regeneration of pulp tissue with capillaries and neuronal cells within 14 days. Gene-expression studies demonstrated the expression of pro-angiogenic factors, implying trophic action on endothelial cells. Conclusions: This investigation demonstrates the potential utility of fractionated SP cells as a source of cells for total pulp regeneration complete with angiogenesis and vasculogenesis.

    Papers of special note have been highlighted as: ▪ of interest ▪▪ of considerable interest

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