Nakazawaea tricholomae sp. nov. , a novel ascomycetous yeast species
isolated from two mushrooms in China
Abstract
Two new yeast strains designated as 20-27-1 and 20-28 were isolated from
the fruiting body of Tricholoma gambosum and Marasmius
maximus, respectively, which were collected in Wudaogou, Weichang
county, Chengde area, Hebei Province, China. Analysis of the sequences
of the D1/D2 domains of the large subunit (LSU) rRNA gene and the
internal transcribed spacer (ITS) regions showed that the two new
strains differed from each other by one nucleotide substitutions in the
D1/D2 domains and were identical in the ITS regions. The combined ITS
and D1/D2 sequences phylogenetic analysis showed that the two strains
were closely related to Nakazawaea ernobii and Nakazawaea
holstii and differed from them by 99.3% (substitution: deletion 3: 1)
and 98.7% (substitution: deletion 6: 1) similarity in D1/D2 domains,
respectively. About 97.1% similarity of ITS sequences was presented
between the two new strains and the two known species. Physiologically,
those two new strains differed from N. ernobii and N.
holstii by assimilation of melibiose, inulin, D-glucosamine, soluble
starch and DL-lactate. The phenotypic and phylogenetic analyses
indicated that those two strains represent a novel species of the genus
Nakazawaea. Therefore, the name Nakazawaea tricholomae sp.
nov. was proposed for those two strains. The holotype is deposited in
China General Microbiological Culture Collection Center as CGMCC 2.7006
T. The new species name is registered in Fungal Names
as FN 571492.