Pesticidi i fitomedicina 2020 Volume 35, Issue 3, Pages: 183-191
https://doi.org/10.2298/PIF2003183C
Full text ( 720 KB)
Assessment of the genotoxic potential of temephos
Cobanoglu Hayal (Çanakkale Onsekiz Mart University, Vocational Health College, Terzioglu Campus, Çanakkale, Turkey), hayaltok@gmail.com
Cayir Akin (Çanakkale Onsekiz Mart University, Vocational Health College, Terzioglu Campus, Çanakkale, Turkey)
Genotoxic effects of pesticides are of great concern for public health due to
the fact that they are widely used for both domestic and industrial
purposes. Temephos is a member of organophosphorus pesticides, which is the
most widely used group of chemicals against both agricultural and domestic
insects. We therefore aimed in the present study to investigate the
genotoxic and cytotoxic effects of temephos on human peripheral blood
lymphocytes, using the cytokinesis-block micronucleus (CBMN) and sister
chromatid exchange assays. The results showed that micronucleus (MN)
frequency increased at concentrations of 50 and 75 μg/ml although it was not
found statically significant (p>0.05). We found that sister chromatid
exchange (SCE) values at concentrations of 50 and 75 μg/ ml were
significantly higher than those obtained for the control (p<0.01). We also
analyzed associations between temephos exposure and mitotic index (MI),
proliferation index (PI), and cell blocked proliferation index (CBPI). There
was no significant change in these values at the tested concentrations
(p>0.05). It can be concluded that temephos was not cytotoxic at
concentrations of 25, 50 and 75 μg/ml. However, it may have a genotoxic
potential in human peripheral lymphocytes.
Keywords: Pesticide exposure, genetic damage, micronucleus, sister chromatid exchange, cytostatic effect