Genetika 2022 Volume 54, Issue 1, Pages: 147-160
https://doi.org/10.2298/GENSR2201147H
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Determination of mutagenic-sensitivity and induced variability in the mutant populations of ‘Bacardi’ chrysanthemum cultivar
Haspolat Gulden (Aegean Agricultural Research Institute, Menemen, Izmir, Turkey), guldenhaspolat@gmail.com
Kunter Burak (Turkish Energy, Nuclear and Mineral Research Agency, Nuclear Energy Research Institute, Saray, Ankara, Turkey)
Kantoglu Yaprak (Turkish Energy, Nuclear and Mineral Research Agency, Nuclear Energy Research Institute, Saray, Ankara, Turkey)
Mutation breeding is one of the most important breeding method for ornamental
plants. Chrysanthemum is the genus that has the richest mutant varieties in
ornamental plants. The objective of this study is to create variation by
gamma irradiation and improving traits by mutation breeding. For this aim,
in vitro bud explants of white Bacardi variety were irradiated by gamma
radiation at 20 Gy (Gray). In vitro subcultures were continued until M1V4
period and observations were obtained in this period. Some changes were
observed on heights and flowers of the plants such as; variable flowers,
flowering time, differentiation on plant length, flower number per bunch and
ray floret differentiations. The changes of the ray florets were determined
as color changes to pink and yellow. Mutation frequency was calculated by
1.1% of the population. Approximately 0.9% of useful mutant lines determined
from the selected mutants.
Keywords: Chrysanthemum, in vitro mutation, effective mutation dose (EMD-LD50), mutation frequency
Show references
AHLOOWALIA, B.S. and M. MALUSZYNSKI (2001): Induced mutations - A new paradigm in plant breeding. Euphytica, 118(2):167-173.
ANDERSON, N.O. (2004): Inheritance of seed set, germination and day neutrality / heat delay insensitivity of garden chrysanthemums (Dendranthema x grandiflora) under glasshouse and field conditions. J. Am. Soc. Hortic. Sci., 129(4):509-516.
BRAKAT, M.N., R.S.A. FATTAH, M. BADR, M.G. EL-TORKY (2010): In vitro mutagenesis and identification of new variants via RAPD markers for improving Chrysanthemum morifolium. Af. J. Agric. Res., 5(8):748-757.
DATTA, S.K., P. MISRA, A.K. MANDAL (2005): In vitro mutagenesis - a quick method for establishment of solid mutant in chrysanthemum. Current Sci., 88(1):155-158.
DATTA, S.K. (2009): A Report on 36 Years of Practical Work on Crop Improvement Through Induced Mutagenesis. Q.Y. Shu (ed.), Induced Plant Mutations in the Genomics Era. Food and Agriculture Organization of the United Nations, Rome, 253-256.
DATTA, K. (2014): Induced mutagenesis: basic knowledge for technological success. In (Ed:N.B. Tomlekova, M.I. Kozgar and M.R.Wani) Mutagenesis Exploring Genetic Diversity of Crops. Wageningen Academic Publishers, 97-139.
HASPOLAT, G., U. SENEL, Y. TANER KANTOGLU, B. KUNTER, N. GUNCAG (2019): In vitro mutation on chrysanthemums. Acta Horticulturae, 1263:261-266
KAUL, A., S. KUMAR, M, THAKUR, M. GHANI (2011): Gamma Ray-Induced in vitro Mutations in Flower Colour in Dendranthema grandiflora Tzelev. Floriculture and Ornamental Biotechnology, 5(1):70-73.
KAZAZ, S., T. KILIC, E. DOGAN, Y. YALCIN MENDI, O. KARAGUZEL (2020): Situation of Present and Future in Ornamental Plants Production. IX. Turkey Agriculture Engineering Technical Congress Book. 673-698.
KHARKWALL, M. (2017): Mutation Breeding for Crop Improvement. Geography and You, 102:27-32.
KORNERUP, A., J.H. WANSCHER, D. PAVEY (1978): Methuen Handbook of Colour. ISBN 0-413-33400-7
KUMAR, B.P.V., S. KUMAR, S. VIJAYA, M. THAKUR (2012): In vitro mutation induction and selection of Chrysanthemum (Dendranthema Grandiflora Tzelev) lines with improved resistance to Septoria Obesa Syd. Int. J. Plant Res., 2(4):103-107.
KUNTER, B., K.Y. KANTOGLU, H. PESKIRCIOGLU, Z. SAGEL, I. TUTLUER, M. OZCOBAN, N, GUNCAG (2016): Development of Mutation Breeding and Agronomic and Morphological Features in Some Fruit and Ornamental Plants Types in Turkey, Oral Presentation, 11th National Congress of Nuclear Sciences and Technologies, 12 -14.
MANDAL, A.K.A., D. CHAKRABARTY, S.K. DATTA (2000a): In vitro isolation of solid novel flower colour mutants from induced chimeric ray florets of chrysanthemum. Euphtica, 114:9-12.
MANDAL, A.K.A., D, CHAKRABARTY, S.K. DATTA (2000b): Application of in vitro techniques in mutation breeding of chrysanthemum. Plant cell, Tissue and Organ Culture, 60:33-38.
MILER, N. and I.,JĘDRZEJCZYK (2018): Chrysanthemum plants regenerated from ovaries: a study on genetic and phenotypic variation. Turkish J. Bot., 42: 289-297.
MELSEN, K., M. VAN DE WOUW, R. CONTRERAS (2021): Mutation Breeding in Ornamentals, American Society of Horticultural Science. Mutation breeding in ornamentals, 56(10):1154-1165.
MURASHIGE, T. and F. SKOOG (1962): A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum, 15:473-479.
NAGATOMI, S. and K. DEGI (2009): Mutation Breeding of Chrysanthemum by Gamma Field Irradiation and In Vitro Culture. In (Ed:Q.Y. Shu) Induced Plant Mutations in the Genomics Era. Food and Agriculture Organization of the United Nations, Rome, 258-261.
NENCHEVA, D. (2010): In vitro propagation of chrysanthemum. In (Ed: S. Mohan Jain Sergio, J. Ochatt) Protocols for In Vitro Propagation of Ornamental Plants, 177-185.
OLADOSU, Y., M.Y. RAFII, N., ABDULLAH, G., HUSSIN, A., RAMII, H.M., RAHIM, G., MIAH, M., USMAN (2016): Principle and application of plant mutagenesis in crop improvement: a review. Biotech. Biotech. Equip., 30:1-16.
ROHLF, F.J. (2000): NTSYSpc: Numerical Taxonomy And Multivariate Analysis System, Version 2.1. Exeter Software, NY
ROUT, G.R. and P., DAS (1997): Recent trends in the biotechnology of Chrysanthemum: a critical review. Scientia Horticulturae, 69(3-4):239-257.
SARSU, F., S. PENNA, B. KUNTER, R. IBRAHIM (2018): Mutation Breeding for Vegetatively Propagated Crops in: Manual on Mutation Breeding Third Edition (Ed.) Spencer-Lopes, M.M. Forster, B.P. and Jankuloski, L. Plant Breeding and Genetics Subprogramme Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria Food and Agriculture Organization of the United Nations Vienna, ISBN 978-92-5-130526-3:157-174.
SCHUM, A. (2003): Mutation Breeding in Ornamentals: An Efficient Breeding Method. XXI International Eucarpia Symposium on Classical versus Molecular Breeding of Ornamentals - Part I. ActaHortic.,612(6):47-60.
SUSILA, E., A. SUSILOWATI, A. YUNUS (2019): The morphological diversity of Chrysanthemum resulted from gamma ray irradiation. Biodiversitas, 20(2):463-467.
TEIXEIRA DA SILVA, J.A. and D. KULUS (2014): Chrysanthemum biotechnology: discoveries from the recent literature. Folia Horticulturae, 26(2):67-77.
VAN HARTEN, A.M. (1998): Mutation Breeding Theory and Practical Applications. Cambridge University Press, ISBN 0521470749, 353.
VAN HARTEN, A.M. (2002): Mutation breeding of vegetatively propagated ornamentals. In (Ed: A. Vainstein) Breeding for Ornamentals: Classical and Molecular Approaches. Kluwer Academic Publishers, 155-127.
YAMAGUCHI, H. (2018): Mutation breeding of ornamental plants using ion beams. Breeding Sci., 68:71-78.
ZALEWSKA, M., A. TYMOSZUK, N. MILER (2011): New chrysanthemum cultivars as a result of in vitro mutagenesis with the application of different explant types. Acta Sci. Pol., Hortorum Cultus, 10(2):109-123.