Joining between Nylon-film and Silicone-rubber was successfully developed by using homogeneous EB irradiation. Effects of electron beam (EB) irradiation on joining strength of different polymers without adhesive materials were investigated. Although excess EB-irradiation more than 300 kJ kg^-1(kGy) apparently decreased the joining strength, the EB irradiation increased the joining strength of the joining sample between Nylon-film and Silicone-rubber. To discuss the influences of EB irradiation on the joining strength, electron spin resonance (ESR) signals related to dangling bonds were observed. Since EB irradiation generated dangling bonds on Nylon-film, dangling bonds probably supported the each other polymer at interface.