OPTICAL RESOLUTION OF 2, 6-DIAMINOPIMELIC ACID STEREOISOMERS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR THE CHEMOTAXONOMY OF ACTINOMYCETE STRAINS

YOKO TAKAHASHI; YUZURU IWAI; HIROSHI TOMODA; NORIYUKI NIMURA; TOSHIO KINOSHITA; SATOSHI OMURA

doi:10.2323/jgam.35.27

YOKO TAKAHASHI, YUZURU IWAI, HIROSHI TOMODA, NORIYUKI NIMURA, TOSHIO KINOSHITA, SATOSHI OMURA

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JOURNAL FREE ACCESS

1989 Volume 35 Issue 1 Pages 27-32

DOI https://doi.org/10.2323/jgam.35.27

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Abstract

For the chemotaxonomy of actinomycetes, we describe the optical resolution method for 2, 6-diaminopimelic acid (DAP) stereoisomers by high performance liquid chromatography (HPLC) using 2, 3, 4, 6-tetra-O-acetyl- β-D-glucopyranosyl isothiocyanate (GITC). The analysis was applicable both to whole-cell preparations and to cell wall ones. This method made it possible to analyze quantitatively LL-, DD-, and meso-DAP, compared with the previously reported methods of paper chromatography, thin-layer chromatography and HPLC.

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