The goal of this research is to generate high quality chromatic Contrast Sensitivity Function (CSF) over a wide range of spatial frequencies from 0.06 to 3.84 cycles per degree (cpd) surrounding 5 CIE proposed colour centres (white, red, yellow, green and blue) to study colour difference. At each centre, 6 colour directions at each of 7 frequencies were sampled, from 0.06 to 3.84 cycles per degree (cpd) corresponding to the number of cycles: from 2.3 to 144.4 respectively. A threshold method based on forced-choice stair-case was adopted to investigate the just noticeable (threshold) colour difference. The results revealed that the chromatic CSF under the present experimental conditions having many lower spatial frequencies covering five colour centres to be band pass, whereas previous results indicated it was low pass. However, this could be caused by the present experimental conditions such as fixed-size stimuli and constant luminance. The new chromatic CSF for R-G and Y-B channels were also developed.