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Fluorescence in situ hybridisation

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Abstract

Fluorescence in situ hybridisation (FISH) is a rapid and reliable technique for chromosomal investigations that is used for a wide variety of cytogenetic purposes at present. This molecular-cytogenetic method has been developed continuously for many years. As a consequence, various modifications with different kinds of fluorescently labelled probes have been introduced to optimise the detection of DNA and RNA sequences. This review articlepaper presents the general principles of in situ hybridisation, probe labelling and examples of proper use of different kinds of probes. In addition, some newer FISH methods and their usefulness in human molecular cytogenetics are described.

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Abbreviations

CCK:

colour changing karyotyping

CGH:

comparative genomic hybridisation

DIG:

digoxigenin

FISH:

fluorescence in situ hybridisation

M-FISH:

multicolour (multiplex) FISH

PNA:

polypeptide nucleic acid

SCAN:

Spectral colour banding

SKY:

spectral karyotyping

WCP:

whole chromosome painting

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  1. Institute of Veterinary Genetics, University of Veterinary Medicine, Komenského 73, SK-04181, Košice, Slovakia

    Martina Lakatošová & Beáta Holečková

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  1. Martina Lakatošová
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Lakatošová, M., Holečková, B. Fluorescence in situ hybridisation. Biologia 62, 243–250 (2007). https://doi.org/10.2478/s11756-007-0043-2

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