Abstract
A highly efficient protocol for in vitro regeneration of an indigenous, endangered medicinal plant Celastrus paniculatus was achieved using nodal explants. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) showed maximum percentage of shoot multiplication (83.4%) with 8.2 shoots/explants. Maximum rooting of 73.3% with 4.8 roots/shoot was achieved on half-strength MS media supplemented with 0.5 mg/L indole-3-acetic acid (IAA) and the percentage of survival was 91% after acclimatization. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) marker study confirmed genetic stability for in vitro raised explants by showing 100% monomorphism. High multiplication rate associated with genetic stability ensure the efficacy of the present in vitro clonal propagation protocol of this important medicinal plant species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bantawa P., Saha-Roy O., Kumar S., Ghosh R. & Mondal K.T. 2011. In vitro regeneration of an endangered medicinal plant Picrorhiz scrophulariiflora. Biol. Plant. 55: 169–172.
Chandrika M. & Rai V.R. 2009. Genetic fidelity in micropropagated plantlets of Ochreinauclea missionis an endemic, threatened and medicinal tree using ISSR markers. Afr. J. Biotechnol. 8: 2933–2938.
De Silva M.A.N. & Senarath W.T.P.S.K. 2009. Development of a successful protocol for in vitro mass propagation of Celastrus paniculatus Willd. — a valuable medcinal plant. Tropical Agricult. Res. 21: 21–29.
Doyle J.J. & Doyle J.L. 1990. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.
Eshraghi P., Zarghami R. & Ofoghi H. 2005. Genetic stability of micropropagated plantlets in Date Palm. J. Sci. 16: 311–315.
Gopi C. & Nataraja, S.Y. Ponmurugan P. 2006. In vitro multiplication of Ocimum gratissimum L. through direct regeneration. Afr. J. Biotechnol. 5: 723–726.
Harter H.L. 1960. Critical values for Duncan’s multiple range test. Biometric. 16: 671–685.
Kaeppler S.M., Kaeppler H.F. & Rhee Y. 2000. Epigenetic aspects of somaclonal variation in plants. Plant Mol. Biol. 43: 179–188.
Lal D. & Singh N. 2010. Mass multiplication of Celastrus paniculatus Wild: An important medicinal plant under in vitro conditions via nodal segments. Int. J. Biodiv. Conserv. 2: 140–145.
Larkins P. & Scowcroft W.R. 1981. Somaclonal variation, a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60: 197–214.
Martins M., Sarmento D. & Oliveira M.M. 2004. Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers. Plant Cell Rep. 23: 492–496.
Mohanty S., Panda M.K., Sahoo S. & Nayak S. 2011. Micropropagation of Zingiber rubens and assessment of genetic stability through RAPD and ISSR markers. Biol. Plant. 55: 16–20.
Murashige T. & Skoog F.A. 1962. Revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497.
Murthy S.R., Kondamudi R. & Vijayalakshmi V. 2010. Micropropagation of an endangered medicinal plant Ceropegia spiralis L. J. Agri. Technol. 6: 179–191.
Premvaranon P., Vearasilp S., Thanapornpoong S., Karladee D. & Gorinstein S. 2011. In vitro studies to produce double haploid in Indica hybrid rice. Biologia 66: 1074–1081.
Rao M.S. & Purohit, S.D. 2006. In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd. Biol. Plant. 50: 501–506.
Rekha K., Bhan M.K., Balyan S.S. & Dhar A.K. 2005. Cultivation prospects of endangered species Celastrus paniculatus Willd. Nat. Prod. Rad. 4: 483–486.
Rout G.R., Mahato A. & Senapati S.K. 2008. In vitro clonal propagation of Nyctanthes arbor-tristis. Biol. Plant. 52: 521–524.
Sharada M., Ashok A. & Kaul M.K. 2003. Regeneration of plantlets via callus cultures in Celastrus paniculatus Willd. — A rare endangered medicinal plant. J. Biochem. Biotec. 12: 65–69.
Sharma P.C., Yelne M.B. & Dennis T.J. 2001. Database on Medicinal Plants used in Ayurveda. Central Council for Research in Ayurveda and Sidda. Janakpori, New Delhi, 636 pp.
Swarna J. & Ravindhran R. 2012. In vitro propagation and assessment of genetic integrity of Talinum triangulare (Jacq.) Wild: a valuable medicinal herb. Acta Physiol Plant. 10: 1007–1017
Tantikanjana T., Young W.H.J., Letham D.S., Grifith M., Hussain M. & Ljung K. 2001. Control of auxillary budproliferation and shoot architecture in Arabidopsis through Supershoot gene. Genes Dev. 15: 1577–1588.
Vendrame W.A., Kochert G. & Wetzstein H.Y. 1999. AFLP analysis of variation in pecan somatic embryos. Plant Cell Rep. 18: 853–857.
Walia N., Sinha S. & Babbar S.B. 2003. Micropropagation of Crataeva nurvala. Biol. Plant. 46: 181–185.
Williams J.G.K., Kubelik A.R., Liva K.J., Rafalski J.A. & Tingey S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18: 6531–6535.
Zeitkiewicz E., Rafalski A. & Labuda D. 1994. Genome finger printing by simple sequence repeat (SSR)-anchored PCR amplification. Genomics. 20: 176–183.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Senapati, S.K., Aparajita, S. & Rout, G.R. Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant. Biologia 68, 627–632 (2013). https://doi.org/10.2478/s11756-013-0187-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2478/s11756-013-0187-1