Isolation of CA 125 from Breast Tumor Tissue Using Gel Chromatography

Authors

  • Nazar Ahmed Naji

DOI:

https://doi.org/10.25130/tjphs.2007.2.12.170.182

Abstract

Gel filtration chromatography technique was used (Sepharose CL - 68) of CA 125 from malignant breast tumor homogenate and the isolation of (CA 125 I 1251 - anti CA 125 antibody), complex was prepared from crude CA 125 , isolated CA 125 and standard human CA 125. The results revealed the presence of two forms of CA 125 (Agl & AgII) and found to be 517 Kda and 38 Kda respectively; The isolation of CA 125 shov,:ed a purification fold of 17.5 Agl and 15 for AgII.

Downloads

Published

2023-04-09

How to Cite

1.
Ahmed Naji N. Isolation of CA 125 from Breast Tumor Tissue Using Gel Chromatography. Tikrit J. Pharm. Sci. [Internet]. 2023 Apr. 9 [cited 2024 May 16];3(2):170-82. Available from: https://tjphs.tu.edu.iq/index.php/j/article/view/112