Analytical method of deoxynivalenol, nivalenol and fusarenon-X in cereals
1984 Volume 1984 Issue 19 Pages 34-37
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1984 Volume 1984 Issue 19 Pages 34-37
A simple and sensitive method for chemical analyses of three trichothecene mycotoxins (deoxynivalenol, nivalenol and fusarenon-X) has been developed. Extraction of the toxins from sample with 200 ml of aqueous acetonitrile (acetonitrile: water=150:50) was followed by clean-up using a Florisil column and a Sep-pak silica cartridge, and then determination of the toxins were carried out with GC (ECD) and GC-MS (mass fragmentography). The detection limits of deoxynivalenol, nivalenol and fusarenon-X were 2, 2 and 4 ppb respectively, when the method was applied to rice, wheat, corn and soybean.
