Genotypic Characterization of Antibiotic-Resistant Genes in Gram Negative Bacteria Isolated from Selected Fish Ponds Effluents Samples within Oyo State
doi.org/10.26538/tjnpr/v6i8.24
Keywords:
Antibiotics resistance,, Carbapenemese,, ESBL,, Fishpond effluents.Abstract
Extended-spectrum beta-lactamase (ESBL) and carbapenem-resistant bacteria are becoming a rising global public health risk, with food products serving as distribution channels and aquatic ecosystems as prospective storage. The focus of this research therefore is to isolate antibiotics resistant Enterobacteriaceae from selected fish pond effluents in Oyo State. A total of 129 effluents were collected from 42 fish ponds and were cultivated on MacConkey agar. Bacteria were isolated and antibiotic susceptibility of the isolates to Gentamicin, imipenem, meropenem, tetracycline, and cefepime was determined using the disc diffusion method. MDR bacteria were checked for blaCTX-M, blaSHV, blaTEM, blaKPC, blaOXA, and blaNDM resistance genes using polymerase chain reaction (PCR), and isolate with resistance genes were characterized using 16S rRNA sequencing. Forty-six point five (46.5) percent of the 270 Enterobacteriaceae isolates obtained from effluent samples were resistant to imipenem, meropenem (45.7%), and tetracycline (39.4%), cefepime (35.8%), and gentamycin (19.2%). blaSHV was the sole gene found in 13.33 % of the isolates examined by polymerase chain reaction. The detection of ESBL and carbapenemase-producing gram-negative bacteria from selected fish ponds in this study is confirmed and represents a major public health problem. As a result, regular surveillance of antibiotic-resistant bacteria in fish ponds is required to aid disease control and better understand their public health implications.
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