Acute and Subchronic Toxicity Study of Methanol Extract of the Aerial Parts of Ruta montana L. on Adult Female Wistar Rats
doi.org/10.26538/tjnpr/v6i8.7
Keywords:
Ruta montana L.,, Female rats,, LD50,, Subchronic toxicity,, Reproductive hormoneAbstract
Ruta montana L. locally known as fidjel belongs to the rutaceae family. This plant is used in traditional medicine as an aphrodisiac, abortive and as hypoglycemic. The aim of this research is to evaluate the mean lethal dose (LD50) and subchronic toxicity of methanol extract of Ruta montana L. on female rat. To determine the LD50, 30 female rats were divided into 5 groups; each group received intraperitoneally, a single dose (800, 1000, 1200, 1400 and 1600 mg/kg bw) of the extract, toxicity symptoms and mortality were registered for 14 consecutive days. In subchronic toxicity (45 days), 14 female rats were divided into 2 groups; treated group received intraperitoneally 38,7149 mg/kg bw = 1/30 LD50 of the extract and control group received physiological water. Body weight, relative organ masses, reproductive hormones, haematological, biochemical and histopathological parameters were evaluated. The LD50 value was calculated to be 1161.4486 mg/kg by Litchfield and Wilcoxon method. In the subchronic study, there was a significant reduction in body weight in the 2nd and 7th week (P<0.05). Relative mass of the brain and ovaries registered a significant decrease and increase, respectively(P<0.05). Follicle stimulating hormone (FSH), luteinizing hormone (LH) and biochemical parameters did not record significant difference. Haematological parameters presented significant increase and decrease in mean corpuscular haemoglobin (MCH) and red cell distribution width (RDW), respectively (P<0.05). The histopathology revealed no abnormalities in different studied tissues. The current study revealed that the methanol extract of Ruta montana at the tested dose had no toxic effect on subjected organs.
References
Almasad MM, Qazan WS, Daradka H. Reproductive toxic effects of Artemisia herba alba ingestion in female Spague- dawley rats. Pak J Biol Sci. 2007; 10(18):3158-3161.
Qazan WS. Effects of short and long term treatment of Ballota undulate on female albino rats fertility and pregnancy. Pak J Biol Sci. 2008; 11(4):638-642.
Usman MM, Sule MS, Gwarzo MY. Toxicological studies of aqueous root extract of Euphorbia lateriflora: (Schum and Thonn) in rats. J Med Plants Stud. 2014; 2(2):58-62.
Kpemissi M, Metowogo K, Melilaa M, Veerapurc VP, Negru M, Taulescu M, Potarniche AV, Suhas DS, Puneeth TA, Vijayakumar S, Gadegbeku KE, Aklikokou K. Acute and subchronic oral toxicity assessments of Combretum micranthum (Combretaceae) in Wistar rats. Toxicol Rep. 2020; 7:162-168.
Bonnier G and Douin R. The great flora in color by Gaston Bonnier. Bellin: Paris; 1990; 205p.
Harsha SN and Latha BV. In vitro antioxidant and in vitro anti-inflammatory activity of Ruta graveolens methanol extract. Asian J Pharm Clin Res. 2011; 5(1):32-35.
Bouzidi MA, Faraoun F, Meliani H, Boubekeur I, Kralifa M, Zattal Z. Characterization of the soil occupied by Ruta genus species in Tessala mountain (north-west of Algeria). Lab. Technol. 2012; 7(28):79-84.
Zellagui A, Belkassam A, Belaidi A, Gherraf N. Environmental impact on the chemical composition and yield of essential oils of Algerian Ruta montana (Clus.) L and their antioxidant and antibacterial activities. Adv Environ Biol. 2012; 6(10):2684-2688.
Kara Ali W, Ihoual S, Abidli N. Antioxidant and MDR reversal activity in resistant human ovarian cancer cells of methanolic extract from Ruta montana located in the North of Algeria. Der Pharma Chemica. 2016; 8(12):215-223.
Masri W, Belwaer I, Khlifi F, Nouioui A, Ben salah D, Amira D, Hedhili A. Acute poisoning by Ruta montana: A case report. Phytothérapie. 2015; 13(1):36-38.
Bellakhdar J, Claisse R, Fleurentin J, Younos C. Reportory of standard herbal drugs in the Moroccan pharmacopoeia. J Ethnopharmacol. 1991; 35(2):123-143.
Kambouche N, Merah B, Bellahouel S, Bouayed J, Dicko A, Derdour A, Younos C, Soulimani R. Chemical composition and antioxidant potential of Ruta montana L. Essential oil from Algeria. J Med Food. 2008; 11(3):593-595.
Pollio A, De Natale A, Appetiti E, Aliotta G, Touwaide A. Continuity and change in the mediterranean medical tradition: Ruta spp. (rutaceae) in Hippocratic medicine and present practices. J Ethnopharmacol. 2008; 116(3):469-482.
Benkhnigue O, Ben Akka F, Salhi S, Fadli M, Douira A, Zidane L. Catalog of medicinal plants used in the treatment of diabetes in the region of Al Haouz-Rhamna (Morocco). J Anim Plant Sci. 2014; 23(1):3539-3568.
Ouarghidi A, Gary JM, Abbad A. Botanical identification and ethno-medicinal uses of some underground part of medicinal plants collected and traded in Marrakech region. J Med Plants Res. 2013; 7(29):2165-2169.
Bnouham M, Mekhfi H, Legssyer A, Ziyyat A. Medicinal plants used in the treatment of diabetes in Morocco. Int J Diabetes Metab. 2002; 10(1):33-50.
Lakhdar L. Evaluation of the antibacterial activity of Moroccan essential oils on aggregatibacter actinomycetemcomitans: in vitro study. Mohamed V University of Rabat. 2015; 18p.
Eddouks M, Maghrani M, Lemhadri A, Ouahidi ML, Jouad H. Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the south-east region of Morocco (Tafilalet). J Ethnopharmacol. 2002; 82(2-3):97-103.
Daoudi A, Hrouk H, Belaidi R, Slimani I, Ibijbijen J, Nassiri L. Valorization of Ruta montana and Ruta chalepensis: Ethnobotanical study, phytochemical screening and Antibacterial activity. J Mater Environ Sci. 2016; 7(3):926- 935.
Sofowora A. Medicinal plants and traditional medicine of Africa. Karthala; 2010; 25p.
Litchfield JRJT, Wilcoxon f. A simplified method of evaluating dose-effect experiments. J Pharmacol Exp Ther. 1949; 96 (2):99-113.
Alturkistani HA, Tashkandi FM, Mohammedsaleh ZM. Histological Stains: A literature review and case study. Glob J Health Sci. 2015; 8(3):72-79.
Alelign T, Chalchisa D, Fekadu N, Solomon D, Sisay T, Debella A, Petros B. Evaluation of acute and sub-acute toxicity of selected traditional antiurolithiatic medicinal plant extracts in wistar albino rats. Toxicol Rep. 2020; 7:1356-1365.
Kale OE, Awodele O, Akindele AJ. Subacute and subchronic oral toxicity assessments of Acridocarpus smeathmannii (DC.) Guill. & Perr. root in wistar rats. Toxicol Rep. 2019; 6:161- 175.
Takhtajan A. Flowering Plants. (2nd ed). Springer: Russia; 2009; xli p.
Hammiche V, Merad R, Azzouz M. Poisonous plants for medicinal use around the Mediterranean. Springer: Paris; 2013; 212p.
Ogbuehi IH, Ebong OO, Obianime AW. Oral acute toxicity (LD50) study of different solvent extracts of Abrus precatorius Linn leaves in wistar rats. Eur J Exp Biol. 2015; 5(1):18-25.
Allouni R. Study of the morphological, phytochemical and pharmacotoxicological aspects of the Ruta montana plant. Farhet Abbas, Setif University.2018; 60p.
Chollet S, Papet Y, Mura P, Brunet B. Determination of atropine and scopolamine contents in wild and ornamental varieties of Datura. Ann Anal Toxicol. 2010; 22 (4):173-179.
Gadanya AM, Sule MS, Atiku MK. Acute toxicity study of “gadagi” tea on rats. Bayero J Pure Appl Sci. 2011; 4 (2):147- 149.
Zbinden G and Flury-Roversi M. Significance of the LD50 test for the toxicological evaluation of chemical substances. Arch Toxicol. 1981; 47(2):77-99.
Loomis TA and Hayes WA. Loomis's Essentials of Toxicology. (4th ed). Academic Press: London; 1996; 25p.
Sharif HB, Mukhtar MD, Mustapha Y, Gabi B, Lawal AO. Acute and Subchronic Toxicity Profile of Euphorbia pulcherrima methanol extract on wistar Albino rats. Adv Pharm. 2015; 2015:1-9.
Tédong L, Dzeufiet PDD, Dimo T, Asongalem AE, Sokeng DS, Flejou JF, Callard P, Kamtchouing P. Acute and subchronic toxicity of Anacardium occidentale linn (anacardiaceae) leaves hexane extract in mice. Afr J Trad CAM. 2007; 4 (2):140-147.
Ghelani H, Chapala M, Jadav P. Diuretic and antiurolithiatic activities of an ethanolic extract of Acorus calamus L. rhizome in experimental animal models. J Trad Compl Med. 2016; 6(4):431-436.
Benziane MM. Phytochemical screening of the Ruta montana plant. Extraction of essential oil and rutin. Antioxidant activity of the plant. Es-Senia University of Oran. 2007; 48p.
Patel C, Dadhaniya P, Hingorani L, Soni MG. Safety assessment of pomegranate fruit extract: Acute and subchronic toxicity studies. Food Chem Toxicol. 2008; 46 (8):2728-2735.
Rockett JC, Narotsky MG, Thompson KE, Thillainadarajah I, Blystone CR, Goetz AK, Rena H, Best DS, Murrell RN, Nichols HP, Schmid JE, Wolf DC, Dix D J. Effect of conazole fungicides on reproductive development in the female rat. Reprod Toxicol. 2006; 22(4):647-658.
Arsad SS, Esa NM, Hamzah H, Othman F. Evaluation of acute, subacute and subchronic oral toxicity of Rhaphidophora decursiva (Roxb.) Schott extract in male Sprague Dawley rats. J Med Plant Res. 2013; 7(41):3030-3040.
Amna FO, Nooraain H, Noriham A, Azizah AH, Husna NR. Acute and oral subacute toxicity study of ethanolic extract of Cosmos caudatus leaf in sprague dawley rats. Int J Biosci Biochem Bioinforma. 2013; 3(4):301-305.
Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lilly P, Sanders J, Sipes G, Bracken W, Dorato M, Van Deun K, Smith P, Berger B, Heller A. Concordance of the toxicity of pharmaceuticals in humans and in animals. Regul Toxicol Pharmacol. 2000; 32(1):56-67.
Jashni HK, Jahromi HK, Ranjbary AG, Jahromi ZK, Kherameh ZK. Effects of aqueous extract from Asparagus officinalis L. roots on hypothalamic-pituitary-gonadal axis hormone levels and the number of ovarian follicles in adult rats. Int J Reprod BioMed. 2016; 14(2):75-80.
Yaghmaei P, Parivar K, Jalalvand F. Effect of Imatinib on the oogenesis and pituitary –ovary hormonal axis in female Wistar rat. Int J Fertil Steril. 2009; 3(1):11-16.
Monsees TK and Opuwari CS. Effect of rooibos (Aspalathus linearis) on the female rat reproductive tract and liver and kidney functions in vivo. S Afr J Bot. 2017; 110:208-215.
Lilaram and Raichur AN. Effect of ethanolic seed extract of Caesalpinia bonducella on female reproductive system of albino rat: a focus on antifertility efficacy. Asian Pac J Trop Dis. 2012; 2:S957-S962.
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