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Abstract

The advent of COVID-19, has posed a risk that human respiratory samples containing human influenza viruses may also contain SARS-CoV-2. This potential risk may lead to SARS-CoV-2 contaminating conventional influenza vaccine production platforms as respiratory samples are used to directly inoculate embryonated hen’s eggs and continuous cell lines that are used to isolate and produce influenza vaccines. We investigated the ability of these substrates to propagate SARS-CoV-2 and found that neither could support SARS-CoV-2 replication.

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/content/10.2807/1560-7917.ES.2020.25.25.2001122
2020-06-25
2024-04-20
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.25.2001122
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References

  1. Trombetta CM, Marchi S, Manini I, Lazzeri G, Montomoli E. Challenges in the development of egg-independent vaccines for influenza. Expert Rev Vaccines. 2019;18(7):737-50.  https://doi.org/10.1080/14760584.2019.1639503  PMID: 31265333 
  2. Hegde NR. Cell culture-based influenza vaccines: A necessary and indispensable investment for the future. Hum Vaccin Immunother. 2015;11(5):1223-34.  https://doi.org/10.1080/21645515.2015.1016666  PMID: 25875691 
  3. Gregersen JP. A quantitative risk assessment of exposure to adventitious agents in a cell culture-derived subunit influenza vaccine. Vaccine. 2008;26(26):3332-40.  https://doi.org/10.1016/j.vaccine.2008.03.075  PMID: 18485545 
  4. Kim D, Quinn J, Pinsky B, Shah NH, Brown I. Rates of Co-infection Between SARS-CoV-2 and Other Respiratory Pathogens. JAMA. 2020;323(20):2085.  https://doi.org/10.1001/jama.2020.6266  PMID: 32293646 
  5. Matrosovich M, Matrosovich T, Carr J, Roberts NA, Klenk HD. Overexpression of the alpha-2,6-sialyltransferase in MDCK cells increases influenza virus sensitivity to neuraminidase inhibitors. J Virol. 2003;77(15):8418-25.  https://doi.org/10.1128/JVI.77.15.8418-8425.2003  PMID: 12857911 
  6. Lee JM, Huddleston J, Doud MB, Hooper KA, Wu NC, Bedford T, et al. Deep mutational scanning of hemagglutinin helps predict evolutionary fates of human H3N2 influenza variants. Proc Natl Acad Sci USA. 2018;115(35):E8276-85.  https://doi.org/10.1073/pnas.1806133115  PMID: 30104379 
  7. Takada K, Kawakami C, Fan S, Chiba S, Zhong G, Gu C, et al. A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses. Nat Microbiol. 2019;4(8):1268-73.  https://doi.org/10.1038/s41564-019-0433-6  PMID: 31036910 
  8. Caly L, Druce J, Roberts J, Bond K, Tran T, Kostecki R, et al. Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia. Med J Aust. 2020;212(10):459-62.  https://doi.org/10.5694/mja2.50569  PMID: 32237278 
  9. Price OH, Spirason N, Rynehart C, Brown SK, Todd A, Peck H, et al. Report on influenza viruses received and tested by the Melbourne WHO Collaborating Centre for Reference and Research on Influenza in 2018. Commun Dis Intell (2018). 2020;44:44.  https://doi.org/10.33321/cdi.2020.44.16  PMID: 32178606 
  10. Swayne DE, Suarez DL, Spackman E, Tumpey TM, Beck JR, Erdman D, et al. Domestic poultry and SARS coronavirus, southern China. Emerg Infect Dis. 2004;10(5):914-6.  https://doi.org/10.3201/eid1005.030827  PMID: 15200830 
  11. Kaye M, Druce J, Tran T, Kostecki R, Chibo D, Morris J, et al. SARS-associated coronavirus replication in cell lines. Emerg Infect Dis. 2006;12(1):128-33.  https://doi.org/10.3201/eid1201.050496  PMID: 16494729 
  12. Gregersen JP. A quantitative risk assessment of exposure to adventitious agents in a cell culture-derived subunit influenza vaccine. Vaccine. 2008;26(26):3332-40.  https://doi.org/10.1016/j.vaccine.2008.03.075  PMID: 18485545 
  13. Matsuyama S, Nao N, Shirato K, Kawase M, Saito S, Takayama I, et al. Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells. Proc Natl Acad Sci USA. 2020;117(13):7001-3.  https://doi.org/10.1073/pnas.2002589117  PMID: 32165541 
SARS-CoV-2 does not replicate in embryonated hen’s eggs or in MDCK cell lines
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Ian+G+Barr&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Barr Ian G</a>, <a href="/search?value1=Cleve+Rynehart&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rynehart Cleve</a>, <a href="/search?value1=Paul+Whitney&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Whitney Paul</a>, <a href="/search?value1=Julian+Druce&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Druce Julian</a></span>. SARS-CoV-2 does not replicate in embryonated hen’s eggs or in MDCK cell lines. <a href="/content/ecdc">Euro Surveill.</a> 2020;25(25):pii=2001122. <a href="https://doi.org/10.2807/1560-7917.ES.2020.25.25.2001122" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2020.25.25.2001122</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 07 Jun 2020;&nbsp;&nbsp; <span class="generated">Accepted</span>: 25 Jun 2020 </span> </p>
/content/10.2807/1560-7917.ES.2020.25.25.2001122
/content/10.2807/1560-7917.ES.2020.25.25.2001122
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