Escherichia coli and Salmonella sp. on some lettuce in the traditional market of Banyuwangi Regency

Eka Yunita Wulandari (1), Sinta Nuriyah Putri Ayu (2), Nur Lailatul Hanifa (3), Brunika Mega Handayani (4), Adhinda Fisabilla (5)
(1) STIKES Banyuwangi, Indonesia, Indonesia,
(2) STIKES Banyuwangi, Indonesia,
(3) STIKES Banyuwangi, Indonesia, Indonesia,
(4) STIKES Banyuwangi, Indonesia, Indonesia,
(5) STIKES Banyuwangi, Indonesia, Indonesia

Abstract

Escherichia coli is the main pathogenic bacteria which causes diarrhea in humans and the fourth highest cause of death which occurs all age groups. Salmonella sp is the cause of typhoid fever and findings of 350-810 cases each year. Diarrhea and typhoid fever are closely related to the consumption of raw vegetables, such as lettuce. This study aimed to analyze the contamination of E. coli and Salmonella sp on some lettuces in Traditional Markets of Banyuwangi Regency. This research was an analytic descriptive study using a cross-sectional design at the Laboratory of Microbiology, STIKES Banyuwangi from 6 to 11 June 2022 using 6 lettuces as research samples which were selected by accidental sampling. All (100%) lettuce samples in this study were contaminated with >1,100 CFU/ml E. coli. All (100%) samples in this study were positively contaminated by Salmonella sp with various contamination number, such as: 0.82x104; 0.47x104, 1.90x105, 2.05x106, 1.00x105 and 1.25x106 CFU/ml, respectively. All six lettuces obtained from the Traditional Market of Banyuwangi Regency did not meet the microbiological quality specified by SNI, indicating that they were not suitable for  direct (raw) consumption.

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References

Afifah, N., & Pawenang, E. (2019). Kejadian Demam Tifoid pada Usia 15-44 Tahun. HIGEIA : Journal of Public Health Research and Development, 3(2), 263–273. https://doi.org/10.15294/higeia/v3i2/24387

Akil, L., & Ahmad, H. A. (2019). Quantitative Risk Assessment Model of Human Salmonellosis Resulting from Consumption of Broiler Chicken. Diseases, 7(1), 11. https://doi.org/10.3390/diseases7010019

Amrinanto, A. H., Hardinsyah, H., & Palupi, E. (2019). The Eating Culture of The Sundanese: Does The Traditional Salad (Lalapan) Improve Vegetable Intake and Blood β-Carotene Concentration? Future of Food: Journal on Food, Agriculture and Society, 7(2), 1–10. https://doi.org/10.17170/kobra-20190709593

Ardiana, M., Augustin, U., & Advinda, L. (2021). Deteksi Jumlah Bakteri Pada Beberapa Sayuran Segar. Jurnal Prosiding Semnas Bio, 1(1), 71–78. https://doi.org/10.24036/prosemnasbio/vol1/12

Badan Standarisasi Nasional. (2009). Batas Maksimum Cemaran Mikroba dalam Pangan.

Canada, P. H. A. of. (2011). Pathogen Safety Data Sheets: Infectious Substances – Salmonella enterica spp. Government of Canada. https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment/salmonella-enterica.html

Dinas Kesehatan Banyuwangi. (2022). Profil Kesehatan Banyuwangi 2021. Dinas Kesehatan Banyuwangi.

Dinas Kesehatan Provinsi Jawa Timur. (2022). Profil Kesehatan 2021. In Dinas Kesehatan Provinsi Jawa Timur.

Ehuwa, O., Jaiswal, A. K., & Jaiswal, S. (2021). Salmonella, Food Safety, and Food Handling Practices. Foods, 10(5), 1–16. https://doi.org/10.3390/foods10050907

Fatiqin, A., Novita, R., & Apriani, I. (2019). Pengujian Salmonella dengan Menggunakan Media SSA dan E. coli Menggunakan Media EMBA Pada Bahan Pangan. Jurnal Indobiosains, 1(1), 22–29. https://jurnal.univpgri-palembang.ac.id/index.php/biosains

Hadiansyah, N., Junitasari, A., & Gustiana, E. (2021). Analysis of Coliform Bacteria in PAMSIMAS Drinking Water Samples in Kuningan Regency. Jurnal Kartika Kimia, 4(2).

Ibrahim, I., & Sartika, R. A. D. (2021). Faktor-Faktor yang Berhubungan dengan Kejadian Diare pada Siswa Sekolah Dasar di Kabupaten Lebak, Provinsi Banten, Indonesia. Indonesian Journal of Public Health Nutrition, 2(1), 34–43. https://doi.org/10.7454/ijphn.v2i1.5338

Jajere, S. M. (2019). A Review of Salmonella enterica with Particular Focus on The Pathogenicity and Virulence Factors, Host Specificity and Adaptation and Antimicrobial Resistance Including Multidrug Resistance. Veterinary World, 12(4), 504–521. https://doi.org/10.14202/vetworld.2019.504-521

Kartikasari, A. M., Hamid, I. S., Purnama, M. T. E., Damayanti, R., Fikri, F., & Praja, R. N. (2019). Isolasi dan Identifikasi Bakteri Escherichia coli Kontaminan Pada Daging Ayam Broiler Di Rumah Potong Ayam Kabupaten Lamongan. Jurnal Medik Veteriner, 2(1), 66. https://doi.org/10.20473/jmv.vol2.iss1.2019.66-71

Khairunnisa, Hidayat, & Herardi. (2020). Hubungan Jumlah Leukosit dan Persentase Limfosit terhadap Tingkat Demam pada Pasien Anak dengan Demam Tifoid di RSUD Budhi Asih Tahun 2018 - Oktober 2019. Seminar Nasional Riset Kedokteran, 60–69.

Khakim, L., & Rini, C. S. (2018). Identifikasi Eschericia coli dan Salmonella sp. Pada Air Kolam Renang Candi Pari. Medicra (Journal of Medical Laboratory Science Atau Tecnology), 1(2), 84–93. https://medicra.umsida.ac.id/index.php

Khasanah, U., Mahasri, G., & Kusdarwati, R. (2021). Examination of Escherichia coli Bacteria in Blood Cockle Satay (Anadara granosa) Sold at Surabaya Traditional Market, Indonesia. World's Veterinary Journal, 11(1), 79–84. https://doi.org/10.54203/scil.2021.wvj11

Kilonzo-Nthenge, A., & Mukuna, W. (2018). Salmonella and Antimicrobial Resistance in Fresh Produce. In Salmonella - A Re-emerging Pathogen (pp. 75–83). InTech. https://doi.org/10.5772/intechopen.72894

Laila, O. N., Khambali, & Sulistio, I. (2022). Perilaku, Sanitasi Lingkungan Rumah dan Kejadian Demam Tifoid. Jurnal Penelitian Kesehatan Suara Forikes, 13(2), 525–529. https://doi.org/10.33846/sf13247

Leboffe, M., & Pierce, B. (2011). A Photographic Atlas for The Microbiology Laboratory. In Anti-Cancer Agents in Medicinal Chemistry (4th ed., Vol. 8, Issue 6). Morton Publishing. https://doi.org/10.2174/187152008785133128

Niguma, H., Pelayo, J., & Oliveira, T. (2017). Microbiological Evaluation of Lettuce Produced by Conventional and Organic Systems in Farms of Londrina, PR. Semina:Ciencias Agrarias, 38(1), 175–183. https://doi.org/10.5433/1679-0359.2017v38n1p175

Olianovi, N., & Pasaribu, D. M. R. (2017). Menghitung Escherichia coli Fekal dari Air Cucian Selada di Pasar Wilayah Kecamatan Grogol. J. Kedokt Meditek, 23(61), 23–31.

Pablos, C., Romero, A., Diego, A., Corrales, C., Grieken, R., Bascón, I., Pérez-Rodríguez, F., & Marugán, J. (2022). Assessing The Efficacy of Novel and Conventional Disinfectants on Salmonella Cross Contamination During Washing of Fresh-Cut Lettuce and Their Impact on Product Shelf Life. LWT - Food Science and Technology, 162, 1–8. https://doi.org/10.1016/j.lwt.2022.113441

Puspita, I., Qurrotul, N. A., Sumarsono, T., & Andini, A. (2020). Uji Sensitivitas Escherichia coli yang Diisolasi dari Air Sumur Galian Dekat dengan Septic Tank Terhadap Ciprofloxacin. Nasional Conference for Ummah.

Rahayu, W. P., Nurjanah, S., & Komalasari, E. (2018). Escherichia coli: Patogenitas, Analisis, dan Kajian Risiko (Vol. 1, Issue 5). IPB Press.

Rahman, M., Alam, M. U., Luies, S. K., Kamal, A., Ferdous, S., Lin, A., Sharior, F., Khan, R., Rahman, Z., Parvez, S. M., Amin, N., Hasan, R., Tadesse, B. T., Taneja, N., Islam, M. A., & Ercumen, A. (2022). Contamination of Fresh Produce with Antibiotic-Resistant Bacteria and Associated Risks to Human Health: A Scoping Review. International Journal of Environmental Research and Public Health, 19(1), 1–15. https://doi.org/10.3390/ijerph19010360

Rahmi, N. A., Putri, M., Azzahra, F. M., Andini, T. B. N., Fitriana, N., Fitri, S. R., Pitri, N., & Fifendy, M. (2021). Persepsi Masyarakat Terhadap Keberadaan Soil Transmitted Helminths pada Sayuran Mentah. Universitas Negeri Padang, 01, 659–672. https://doi.org/10.24036/prosemnasbio/vol1/85

Rahmiati. (2016). Analisis Bakteri Salmonella pada Kuah Sate Pedagang Kaki Lima. BioLink, 3(1), 31–36.

Ramadhani, R., Dian, L., & Yuliawati, S. (2017). Kualitas Bakteriologis Berdasarkan Keberadaan Salmonella sp Pada Selada (Lactusa sativa). Jurnal Kesmas Jambi (JKMJ), 1(1), 11–18.

Salleh, N., Rasul, G., Hassan, Z., Reezal, A., Isa, S., Nishibuchi, M., & Radu, S. (2003). Incidence of Salmonella spp. in raw vegetables in Selangor, Malaysia. Food Control, 14(7), 475–479. https://doi.org/10.1016/S0956-7135(02)00105-6

Scientific, T. F. (2022a). Eosin Methylene Blue Agar CM0069B. Thermo Fisher Scientific.

Scientific, T. F. (2022b). SS Agar (Modified). Thermo Fisher Scientific.

Stec, J., Kosikowska, U., Mendrycka, M., Stepien-Pysniak, D., Niedzwiedzka-Rystwej, P., Bebnowska, D., Hrynkiewicz, R., Zietara-Wysocka, J., & Grywalska, E. (2022). Opportunistic Pathogens of Recreational Waters with Emphasis on Antimicrobial Resistance — A Possible Subject of Human Health Concern. International Journal of Environmental Research and Public Health, 19, 1–17.

Ummah, K. (2022). Upaya Penanggulangan Diare di Kabupaten Lamongan. Jurnal Ilmiah Obsgin, 14(2), 110–113.

Wiastari, N., & Sujaya, I. (2021). Aplikasi Metode Pencucian Terhadap Penurunan Jumlah Bakteri Patogen Pada Sayuran Segar Selada (Lactuca sativa L): Systematic Review. Arc. Com. Health, 8(2), 216–236.

Wibowo, I. S., Wahyunitisari, M. R., & Umiastuti, P. (2017). Deteksi Escherichia coli pada Sayur Lalap di Sekitar Kampus A Universitas Airlangga. JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga, 9(1), 37–41.

Yeni, F., Yavaş, S., Alpas, H., & Soyer, Y. (2016). Most Common Foodborne Pathogens and Mycotoxins on Fresh Produce: A Review of Recent Outbreaks. Critical Reviews in Food Science and Nutrition, 56(9), 1532–1544. https://doi.org/10.1080/10408398.2013.777021

Authors

Eka Yunita Wulandari
ekayunita@stikesbanyuwangi.ac.id (Primary Contact)
Sinta Nuriyah Putri Ayu
Nur Lailatul Hanifa
Brunika Mega Handayani
Adhinda Fisabilla
Author Biography

Sinta Nuriyah Putri Ayu, STIKES Banyuwangi

Teknologi Laboratorium Medis

Wulandari, E. Y., Ayu, S. N. P., Hanifa, N. L., Handayani, B. M., & Fisabilla, A. (2023). Escherichia coli and Salmonella sp. on some lettuce in the traditional market of Banyuwangi Regency. Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya, 8(01). https://doi.org/10.30651/jqm.v8i01.17265

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