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Modern biotechnology is useful in the estimation of the genetic diversity of Ukrainian varieties of hop (Humulus Lupulus L.)

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Abstract

There are two important stages that help form national variety resources: estimation of the varieties of a hop’s gene fund by DNA typing of highly variable microsatellite loci and optimization of the conditions for introducing different genotypes in culture in vitro. These stages constitute the basis for a modern nursery and the means of a legal remedy of proprietorship on a particular variety. Furthermore, these stages are necessary for the development of molecular methods of selection of pathogen-free planting material.

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References

  1. Kostritsya, M.Yu. and Reitman, I.G., Hop and Beer in Ukraine: From Past to Present, Zhitomir. Nauk.-Krazn. Tov. Doslid. Vol., Inst. Sil’sk. Gosp. Polissya, Zhitomir, 1997.

  2. Gladkikh, O. and Shtan’ko, I., Ukrainian Hop Growing: Current State and Prospects, Propozitsiya, 2009, no. 2, pp. 58–68.

  3. Zhigalo, Yu.V., Method for Identification of Hop (Humulus lupulus L.) Cultivars by Morphological Traits, Khmelyarstvo, 2005, no. 22, pp. 17–25.

  4. Polishchuk, I.B. and Zagrafova, M.I., Method of Selection of Initial Hop Breeding Material for the Number of Grains in Generative Organs, in Hop Growing: Collected Papers), Kiev: Urozhai, 1986.

    Google Scholar 

  5. Weising, K., Nybom, H., Wolff, K., and Kahl, G., Fingerprinting in Plants: Principles, Methods and Applications, 2nd ed., CRC Press Taylor and Francis Group, 2005.

  6. Jakse, J. and Javornik, B., High Throughput Isolation of Microsatellites in Hop (Humulus lupulus L.), Plant Mol. Biol. Rep., 2001, vol. 19, pp. 217–226.

    Article  CAS  Google Scholar 

  7. Jakse, J., Satovic, Z., and Javornik, B., Microsatellite Variability among Wild and Cultivated Hops (Humulus lupulus L.), Genome, 2004, vol. 47, pp. 889–899.

    Article  CAS  PubMed  Google Scholar 

  8. Cerenak, A., Jakse, J., and Javornik, B., Identification and Differentiation of Hop Varieties using Simple Sequence Repeat Markers, J. Amer. Soc. Brew. Chem., 2004, vol. 62, no. 1, pp. 1–7.

    CAS  Google Scholar 

  9. Sivolap, Yu.M., Volkodav, V.V., Bal’vins’ka, M.S., Kozhukhova, N.E., Solodenko, A., and Chebotar, S.V., Identification and Registration of Genotyupes of Common Wheat (Triticum aestivum L.), Barley (Hordeum vulgare L.), Maize (Zea mays L.), and Sunflower (Helianthus annuus L.) using Microsatellite Locus Analysis: Guidelines, Odessa: 2004.

  10. Mel’nichuk, M.D., Overchenko, V.V., Spiridonov, V.G., and Parii, M.F., Identification of Hop (Humulus lupulus L.) Genotypes using Microsatellite Markers, Nauk. Dop. NAN Ukrani, 2008, vol. 4, no. 12, pp. 1–12.

    Google Scholar 

  11. Kataeva, N.V. and Butenko, R.G., Klonal’noe mikrorazmnozhenie rastenii (Clonal Microreproduction of Plants), Moscow: Nauka, 1983.

    Google Scholar 

  12. Kalinin, V.L., Kushnir, G.P., and Sarnitskaya, V.V., Tekhnologiya mikroklonal’nogo razmnozheniya rastenii (Technology of Microclonal Reproduction of Plants), Lobov, V.P., Ed., Kiev: Naukova Dumka, 1992.

    Google Scholar 

  13. Guidelines to Hop Growing, Ministry of Agrarian Policy of Ukrain and Ukrainian Academy of Agrarian Sciences, order no. 89/119 from 23.10.2008.

  14. Doyle, J.J. and Doyle, J.L., A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue, Phytochem. Bull., 1987, vol. 19, pp. 11–15.

    Google Scholar 

  15. Krizman, M., Jakse, J., and Baricevic, D., Robust CTAB-Activated Charcoal Protocol for Plant DNA Extraction, Acta Agricult. Sloven., 2006, vol. 87, pp. 427–433.

    CAS  Google Scholar 

  16. Kalendar’, R., Software for Construction of Evolutionary Trees on the Basis of Electrophoregrams of DNA and Proteins, in Molekulyarno-geneticheskie markery i selektsiya rastenii: Dokl. mezhdunar. konf. (Abstracts of Papers, Int. Conf. “Molecular-Genetic Markers and Plant Breeding”), Kiev, 1994, pp. 25–26.

  17. http://www.ncbi.nlm.nih.gov.

  18. http://www.plantgdb.org/.

  19. http://www.chem.qmw.ac.uk.

  20. http://www.fastlane.nsf.gov.

  21. http://www.genome.arizona.edu/fpc/.

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Authors and Affiliations

  1. South Plant Biotechnology Center, National Academy of Agrarian Sciences of Ukraine, ul. Ovidiopol’ska Road 3, Odessa, 650036, Ukraine

    Yu. Sivolap, O. Zakharova, N. Kozhukhova, S. Ignatova, N. Pristavski & G. Zelenina

Authors
  1. Yu. Sivolap
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  2. O. Zakharova
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  3. N. Kozhukhova
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  4. S. Ignatova
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  5. N. Pristavski
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  6. G. Zelenina
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Corresponding author

Correspondence to Yu. Sivolap.

Additional information

Original Ukrainian Text © Yu. Sivolap, O. Zakharova, N. Kozhukhova, S. Ignatova, N. Pristavski, G. Zelenina, 2010, published in Tsitologiya i Genetika, 2010, Vol. 44, No. 5, pp. 3–12.

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Sivolap, Y., Zakharova, O., Kozhukhova, N. et al. Modern biotechnology is useful in the estimation of the genetic diversity of Ukrainian varieties of hop (Humulus Lupulus L.). Cytol. Genet. 44, 263–271 (2010). https://doi.org/10.3103/S0095452710050014

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  • DOI: https://doi.org/10.3103/S0095452710050014

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