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The dynamics of proliferation and differentiation of osteogenic cells under supportive unloading

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Abstract

With the use of radioactive marker of DNA synthesis—3H-thymidine we have studied the dynamics, peculiarities of proliferation and differentiation of osteogenic cells under hind limb unloading of white rats (“tail suspension” method at an angle 35°) during 28 days. The 3H-thymidine was administered at a single dose at the end of the experiment, the biosamples were taken from femoral bones in 1, 48, 96 h. Light and electron-microscopic radioautography with 3H-thymidine (in 1 h) have shown, that basic fraction of DNA synthesizing cells in the zones of adaptive remodelling of bone tissue is represented by little-differentiated perivascular cells (that include osteogenic cell precursors). A tendency for a decrease of a labelling index in the 3H-thymidine osteogenic cells on metaphyseal bone trabeculae under hind limb unloading has been established. The dynamics of labelled cells during various time intervals after 3H-thymidine injection testifies to a delay in the differentiation precursors in osteoblasts and their transformation to osteocytes in experiment animals. The obtained data have shown that a long-term supportive unloading leads to lowering the intensity of osteogenetic processes in long bones and reducing bone mass.

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  1. Institute of Zoology NAS of Ukraine, Kiev, Ukraine

    N. V. Rodionova

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  1. N. V. Rodionova
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Correspondence to N. V. Rodionova.

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Rodionova, N.V. The dynamics of proliferation and differentiation of osteogenic cells under supportive unloading. Cytol. Genet. 45, 80–84 (2011). https://doi.org/10.3103/S0095452711020137

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