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Regeneration of transgenic plants from hairy roots of Cichorium intybus L. var. Foliosum Hegi

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Abstract

Transgenic plants containing either ifn-α2b gene encoding human leukocytic interferon or esxA::fbpBΔTMD genes encoding Mycobacterium tuberculosis antigens ESAT6 and Ag85B were regenerated from hairy root cultures after transformation of chicory cotyledons (Cichorium intybus L. var. Foliosum Hegi) with a wild-type A. rhizogenes A4 strain. The direct shoot regeneration from transgenic roots without callus formation phase on growth regulator-free nutrient medium was demonstrated. The transgenes transfer and transcription in the plants were confirmed by the results of RT-PCR and PCR analyses.

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Authors and Affiliations

  1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kiev, Ukraine

    N. A. Matvieieva, O. M. Kishchenko, A. O. Potrochov, A. M. Shakhovsky & M. V. Kuchuk

Authors
  1. N. A. Matvieieva
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  2. O. M. Kishchenko
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  3. A. O. Potrochov
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  4. A. M. Shakhovsky
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  5. M. V. Kuchuk
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Correspondence to N. A. Matvieieva.

Additional information

Original Russian Text © N.A. Matvieieva, O.M. Kishchenko, A.O. Potrochov, A.M. Shakhovsky, M.V. Kuchuk, 2011, published in Tsitologiya i Genetika, 2011, Vol. 45, No. 5, pp. 11–16.

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Matvieieva, N.A., Kishchenko, O.M., Potrochov, A.O. et al. Regeneration of transgenic plants from hairy roots of Cichorium intybus L. var. Foliosum Hegi. Cytol. Genet. 45, 277–281 (2011). https://doi.org/10.3103/S0095452711050082

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  • DOI: https://doi.org/10.3103/S0095452711050082

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