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Molecular and genetic analysis of soft wheat selection lines with starch of the amylopectin type

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Abstract

A controlled selection of genotypes with null alleles of the Wx genes was carried out in the process of creating soft wheat forms with starch of the amylopectin type, using PCR analysis with primers to Wx loci of the genome of T. aestivum L. A microsatellite analysis of the selected individual plants that are carriers of three null alleles of the Wx genes (Wx-A1b, Wx-B1b, and WxD1b) and a cluster analysis (UPGMA) allowed for picking out four genotypes that were most closely related to the maternal form, i.e., the Kuyal’nik variety.

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References

  1. Starch: Properties and Potential, Galliard, T., Ed., Chichester: Wiley, 1987.

    Google Scholar 

  2. Ribalka, O.I., Chervonis, M.V., and Toporash, I.G., Waxy Wheat with Unique Properties of Starch: Possible Directions of Use, Sber. Pererab. Zerna, 2005, vol. 7, no. 73, pp. 24–28.

    Google Scholar 

  3. Dong Soon Suh, Zihua Ao, and Lau-Lin Iane, Effects of Starch Strusture on Its Application, in Krakhmal i krakhmalosoderzhashchie istochniki—struktura, svoistva i novye tekhnologii: Sb. dokl. mezhdunar. konf. (Starch and Starch-Containing Sources—Structure, Properties, and New Technologies. Proc. Int. Conf.), Moscow, 2001, p. 37.

  4. Zhushman, A.I., Karpov, V.G., Bakulina, L.F., and Buravleva, T.N., Development of Technologies of Dietary Products Based on Starch, in Krakhmal i krakhmalosoderzhashchie istochniki—struktura, svoistva i novye tekhnologii: Sb. dokl. mezhdunar. konf. (Starch and Starch-Containing Sources—Structure, Properties, and New Technologies. Proc. Int. Conf.), Moscow, 2001, p. 160.

  5. Nakamura, T., Hoshino, H., and Hidaka, S., Identification of Three Wx Proteins in Wheat (Triticum aestivum L.), Biochem. Genet., 1993, vol. 31, pp. 75–86.

    Article  PubMed  CAS  Google Scholar 

  6. Nakamura, T., Vrinten, P., Saito, M., and Konda, M., Rapid Classification of Partial Waxy Wheats using PCR-Based Markers, Genome, 2002, vol. 45, pp. 1150–1156.

    Article  PubMed  CAS  Google Scholar 

  7. Petrova, I.V., Khokhlov, O.M., Chebotar, S.V., and Sivolap, Yu.M., The Metrological Characteristics of the Method of Spectrophotometric Determination of Starch Amylose in Grains of Wheat Breeding Lines, Fiziol. Biokhim. Kul’t. Rast., 2010, vol. 42, no. 2, p. 146.

    CAS  Google Scholar 

  8. McLauchlan, A., Ogbonnaya, F.C., and Hollingsworth, B., Development of Robust PCR-Based DNA Markers for Each Homoeo-Allele of Granule-Bound Starch Synthase and Application in Wheat Breeding Programs, Aust. J. Agric. Res., 2001, vol. 52, pp. 1409–1416.

    Article  CAS  Google Scholar 

  9. Röder, M.S., Korsun, V., Wendehake, K., Plaschke, J., Leroy, P., and Ganal, M.W., A Microsatellite Map of Wheat, Genetics, 1998, vol. 49, pp. 2007–2023.

    Google Scholar 

  10. GenePrint STR Systems (Silver Stain Detection), Technical Manual, 2001, No. D004, pp. 1–47.

  11. Lobashov, M.E., Genetika (Genetics), Leningrad: Leningr. Gos. Univ., 1969.

    Google Scholar 

  12. Nei, M. and Li, W.-H., Mathematical Model for Studying Genetics Variation in Terms of Restriction Endonucleases, Proc. Nat. Acad. Sci. U.S.A., 1979, vol. 76, p. 5269.

    Article  CAS  Google Scholar 

  13. Sneath, P.H. and Socal, R.R., Numerical Taxonomy: the Principles and Practice of Numerical Classification, San Francisco: Freeman, 1973.

    Google Scholar 

  14. Kalendar’, R.N., Computer Program for Construction of Evolutionary Trees on the Basis of Electrophoregrams of DNA and Proteins, in Molekulyarno-geneticheskie markery i selektsiya rastenii: Mater. konf. (Molecular-Genetic Markers and Plant Breeding. Proc. Conf.), Kiev, 1994, pp. 25–26.

  15. Korzun, V., Roder M., Ganal M. Genetic Analysis of the Dwarfing Gene (Rht8) in Wheat. Part I. Molecular Mapping of Rht8 in the Short Arm of Chromosome 2D of Bread Wheat (Triticum aestivum L.), Theor. Appl. Genet., 1998, vol. 96, pp. 1104–1109.

    Article  CAS  Google Scholar 

  16. Rebetzke, G.J. and Richards, R.A., Gibberellic Acid-Sensitive Dwarfing Genes Reduce Plant Height to Increase Kernel Number and Grain Yield of Wheat, Aust. J. Agric. Res., 2000, vol. 51, pp. 235–245.

    Article  CAS  Google Scholar 

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Correspondence to I. V. Semenyuk.

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Original Russian Text © I.V. Semenyuk, S.V. Chebotar’, A.I. Rybalka, Yu.M. Sivolap, 2011, published in Tsitologiya i Genetika, 2011, Vol. 45, No. 5, pp. 17–22.

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Semenyuk, I.V., Chebotar’, S.V., Rybalka, A.I. et al. Molecular and genetic analysis of soft wheat selection lines with starch of the amylopectin type. Cytol. Genet. 45, 282–287 (2011). https://doi.org/10.3103/S0095452711050112

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  • DOI: https://doi.org/10.3103/S0095452711050112

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