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Activation of gene expression of the O6-methylguanine-DNA-transferase repair enzyme upon the influence of EMAP II cytokine in human cells in vitro

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Abstract

The aim of the study is to evaluate the effect of recombinant EMAP II cytokine (endothelial and monocyte-activating polypeptide II) on the level of MGMT gene expression; this gene encodes the O6-methylguanine-DNA-methyltransferase (MGMT) repair enzyme in the cell culture of humans. An investigation into the EMAP II effect on the proliferation of cells was carried out using the standard MTT test. The MGMT protein in a cell extract was identified by Western blot analysis. The following cell lines were investigated: A102 (fibroblasts), CB-1 (umbilical cord blood stromal cells), and 4BL6 (cells obtained from peripheral blood). It was shown in these experiments that the EMAP II cytokine induces MGMT expression in human cells of the investigated lines. There was observed a decrease in the quantity of cells in the presence of a high concentration of this cytokine. The level of expression of the MGMT repair enzyme was established to increase in human cells in vitro in a serum-free culture medium with the EMAP II cytokine.

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Authors and Affiliations

  1. Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine

    V. V. Lylo, L. L. Matsevich, E. V. Kotsarenko, L. A. Babenko, A. I. Kornelyuk, E. M. Sukhorada & L. L. Lukash

  2. Shevchenko National University, Kiev, Ukraine

    L. A. Babenko

Authors
  1. V. V. Lylo
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  2. L. L. Matsevich
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  3. E. V. Kotsarenko
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  4. L. A. Babenko
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  5. A. I. Kornelyuk
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  6. E. M. Sukhorada
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  7. L. L. Lukash
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Correspondence to L. A. Babenko.

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Original Russian Text © V.V. Lylo, L.L. Matsevich, E.V. Kotsarenko, L.A. Babenko, A.I. Kornelyuk, E.M. Sukhorada, L.L. Lukash, 2011, published in Tsitologiya i Genetika, 2011, Vol. 45, No. 6, pp. 53–60.

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Lylo, V.V., Matsevich, L.L., Kotsarenko, E.V. et al. Activation of gene expression of the O6-methylguanine-DNA-transferase repair enzyme upon the influence of EMAP II cytokine in human cells in vitro. Cytol. Genet. 45, 373–378 (2011). https://doi.org/10.3103/S0095452711060053

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