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Obtaining the transgenic lines of finger millet Eleusine coracana (L.). with dinitroaniline resistance

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Abstract

The current data is dedicated to the study of bioballistic and Agrobacterium-mediated transformation of finger millet with the constructs carrying the mutant α-tubulin gene (TUAml), isolated from R-biotype goosegrass (Eleusine indica L.), for the decision of problem of dinitroaniline-resistance. It was found that 10 μM of trifluralin is optimal for the selection of transgene plants of finger millet. PCR analysis of transformed lines confirmed the transgenic nature of plants. The analysis of seed of T1 of transgenic lines confirmed heterozygous character of inheritance of trifluralin resistance.

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Authors and Affiliations

  1. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, ul. Osipovs’kogo 2A, Kyiv, 04123, Ukraine

    G. Ya. Bayer, A. I. Yemets & Ya. B. Blume

Authors
  1. G. Ya. Bayer
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  2. A. I. Yemets
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  3. Ya. B. Blume
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Correspondence to G. Ya. Bayer.

Additional information

Original Russian Text © G.Ya. Bayer, A.I. Yemets, Ya.B. Blume, 2014, published in Tsitologiya i Genetika, 2014, Vol. 48, No. 3, pp. 3–11.

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Bayer, G.Y., Yemets, A.I. & Blume, Y.B. Obtaining the transgenic lines of finger millet Eleusine coracana (L.). with dinitroaniline resistance. Cytol. Genet. 48, 139–144 (2014). https://doi.org/10.3103/S0095452714030025

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  • DOI: https://doi.org/10.3103/S0095452714030025

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