Abstract
The ultrastructural organization of loach embryo cells (Misgurnus fossilis L.) at the stage of the first and the tenth embryo divisions was investigated under the influence of low intensity helium-neon laser irradiation of 5 min exposure. It was determined that the effect of laser irradiation led to ultrastructural changes: the shape of mitochondria and multivesicular bodies changes. The obtained results explain the possible mechanism of influence of low-intensity laser irradiation at the cellular level.
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Moshkovska, T. and Mayberry, J., It is time to test low level laser therapy in Great Britain, Postgrad. Med. J., 2005, vol. 81, no. 957, pp. 436–441.
Gavish, L., Perez, L., and Gertz, S.D., Low-level laser irradiation modulates matrix metalloproteinase activity and gene expression in porcine aortic smooth muscle cells, Lasers Surg. Med., 2006, vol. 38, no. 8, pp. 779–786.
Snyder, S.K., Byrnes, K.R., Borke, R.C., et al., Quantitation of calcitonin gene-related peptide mRNA and neuronal cell death in facial motor nuclei following axotomy and 633 nm low power laser treatment, Lasers Surg. Med., 2002, vol. 31, no. 3, pp. 216–222.
Karu, T.I. and Kolyakov, S.F., Exact action spectra for cellular responses relevant to phototherapy, Photomed. Laser Surg., 2005, vol. 23, no. 4, pp. 355–361.
Kipshidze, N., Nikolaychik, V., Keelan, M.H., et al., Low-power helium-neon laser irradiation enhances production of vascular endothelial growth factor and promotes growth of endothelial cells in vitro, Lasers Surg. Meg., 2001, vol. 28, no. 364.
Byrnes, K.R., Wu, X., Waynant, R.W., et al., Low power laser irradiation alters gene expression of olfactory ensheathing cells in vitro, Lasers Surg. Med., 2005, vol. 37, no. 2, pp. 161–171.
Kreisler, M., Christoffers, A.B., Al-Haj, H., et al., Low level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts, Lasers Surg. Med., 2002, vol. 30, no. 5, pp. 365–369.
Stein, A., Benayahu, D., Maltz, L., and Oron, U., Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro, Photomed. Laser Surg., 2005, vol. 23, no. 2, pp. 161–166.
Souza, S.C., Munin, E., Alves, L.P., et al., Low power laser radiation at 685 nm stimulates stem-cell proliferation rate in dugesia Tigesia tigrina during regeneration, J. Photochem. Photobiol. B, 2005, vol. 80, no. 3, pp. 203–207.
Tuby, H., Maltz, L., and Oron, U., Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture, Lasers Surg. Med., 2007, vol. 39, no. 4, pp. 373–378.
Manteifel, V.M., Karu, T.J., and Andreichuk, T.V., A comparative study of chromatin from lymphocyte nuclei upon activation of transcription by irradiation from an He-Ne-laser or phytohemagglutinin, Mol. Biol., 1992, vol. 5, pp. 1054–1062.
Dube, A., Bauer, E., and Bock, C., He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage, Radiat. Environ. Biophys., 2001, vol. 1, pp. 77–82.
Vacca, R.A. and Marra, E., Passarella increase in cytosolic and mitochondrial protein synthesis in rat hepatocytes irradiated in vitro by He-Ne laser, J. Photochem. Photobiol. B, 1996, vol. 3, pp. 197–202.
Chudnovskii, V.M., Leonova, G.N., Skopinov, S.A., et al., Biologicheskie modeli i fizicheskie mekhanizmy lazernoi terapii (Biological Models and Physical Mechanisms of Laser Therapy), Vladivostok: Dal’nauka, 2002.
Karu, T., in Low-Power Laser Therapy, New York: CRC Press, 2003, pp. 4825–4841.
Goida, O.A., Biofizicheskie aspekty rannego ontogeneza zhivotnykh (Biophysical Aspects of Early Ontogenesis of Animals), Kyiv: Naukova Dumka, 1993.
Bura, M., Mandzinets’, S., Temnik, M., et al., Development of embryos and larvae of loach Misgurnus fossilis L. under exposure to low-intensity helium-neon light, Visn. Lviv. Univ., Ser. Biol., 2010, vol. 54, pp. 59–68.
Neifakh, A.A., Molekulyarnaya biologiya protsessov razvitiya (Molecular Biology of Development Processes), Moscow: Nauka, 1977.
Reynolds, E.S., The use of lead citrate at high pH as an electronopaque stain in electron microscopy, J. Cell Biol., 1963, vol. 17, pp. 208–212.
Tselevich, M.V., Kulachkovsky, O.P., and Sanagursky, D.I., Ultrastructural changes in loach (Misgurnus fossilis L.) embryos under borocin treatment, Cytol. Genet., 2004, vol. 38, no. 6, pp. 23–27.
Tselevich, M.V., Features of the ultrastructure of loach embryos under the influence of norfloxacin, Cytol. Genet., 2008, vol. 42, no. 2, pp. 96–100.
Mandzynets, S.M., Kulachkovskii, O.R., and Bura, M.V., Effect of avermectin on the ultrastructural characteristics of loach embryos, Cytol. Genet., 2011, vol. 46, no. 5, pp. 318–323.
Vladimirov, Yu.A., Three hypotheses about the mechanism of action of laser radiation on cells and human body, in Efferentnaya meditsina: Sb. statei (Efferent Medicine: Collected Papers), Moscow: Inst. Biomed. Khim., 1994, pp. 51–67.
Golovchak, N.P., Tarnovs’ka, A.V., Bura, M.V., et al., Prooxidant-antioxidant homeostasis of eel embryos under the influence of low-intensity laser irradiation, Fizika Zhivogo, 2009, vol. 17, no. 1, pp. 76–81.
Romanyuk, M.S., Mandzinets’, S.M., Bura, M.V., and Sanagurs’kii, D.I., Effect of low-intensity laser radiation of varying exposure on the activity of Na,K-ATPase of loach embryos, Fotobiol. Fotomed., 2011, no. 1, pp. 76–83.
Manteifel’, V.M., D’yachkova, L.N., and Karu, T.I., Morphometric study of yeast cells of Torulopsis sphaerica after He-Ne-laser irradiation, Tsitologiya, 2002, no. 12, pp. 1205–1211.
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Original Ukrainian Text © M.S. Romaniuk, M.V. Bura, S.M. Mandzynets, O.R. Kulachkovsky, D.I. Sanagursky, 2014, published in Tsitologiya i Genetika, 2014, Vol. 48, No. 3, pp. 43–47.
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Romaniuk, M.S., Bura, M.V., Mandzynets, S.M. et al. Influence low-intensity laser irradiation on the ultrastructural organization of loach embryo cells. Cytol. Genet. 48, 171–174 (2014). https://doi.org/10.3103/S0095452714030098
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DOI: https://doi.org/10.3103/S0095452714030098