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Superoxide dismutase activity in transgenic canola

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Abstract

Superoxide dismutase (SOD) activity was investigated in leaves of transgenic canola plants which expressed heterologous genes of different origin, namely 1—herbicide resistance genes (bar and simultaneously bar and epsps); 2—DesC desaturase gene (desC) of cyanobacterium Synechococcus vulcanus; 3—human interferon α2b gene (huIFN-α2b); 4—esxA::fbpB ΔTMD fused gene, encoding ESAT-6 and Ag85b Mycobacterium tuberculosis proteins, inducing immune response against tuberculosis; 5—cyp11A1 gene of cytochrome P450SCC from bovine adrenal cortex mitochondria. Introduction of herbicide resistance genes as well as desaturase gene of cyanobacterium and mycobacterium’s genes did not change leaf SOD activity. At the same time it was shown that cyp11A1 and huIFN-α2b canola have increased leaf SOD activity up 58 and 33%, respectively, compared with control ones in non-stress conditions. It may be a prerequisite for improved resistance of these plants to the stressors of different origin.

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Authors and Affiliations

  1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, ul. Academika Zabolotnoho 148, Kyiv, 03680, Ukraine

    L. O. Sakhno & M. S. Slyvets

  2. National Technical University of Ukraine “Kyiv Polytechnic Institute”, pr. Peremohy 37, Kyiv, 03056, Ukraine

    M. S. Slyvets

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  1. L. O. Sakhno
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Correspondence to L. O. Sakhno.

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Sakhno, L.O., Slyvets, M.S. Superoxide dismutase activity in transgenic canola. Cytol. Genet. 48, 145–149 (2014). https://doi.org/10.3103/S0095452714030104

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  • DOI: https://doi.org/10.3103/S0095452714030104

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