Abstract
Cytogenetic analysis of Iris pumila L. plants from different habitats in Ukraine revealed the intraspecific chromosomal polymorphism. The chromosome number 2n = 32 was established for plants of this species. Root apical meristem of I. pumila shoots of all populations showed a high level of mixoploidy. The differences in the percentage of aneuploid cells and anaphase aberration were found between the sampled populations. The anaphase analysis revealed the level of chromosomal rearrangements that reached 9.2% in some populations.
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Bairak, O.M., Shevel’, I.M., Gritsai, I.A., et al., Botanichnii zakaznik “Drabinivka” (Botanical Reserve “Drabinivka”), Poltava: Verstka, 2006.
Parnikoza, I.Yu., Trots’ka, T.B., Trots’kii, M.O., and Kunakh, V.A., The state of Iris pumila L. populations from different regions of Mikolaiv region, in Materiali drugikh naukovikh chitan' pam’yati Sergiya Tarashchuka (Proc. Scientific Readings in Memory of Sergei Tarashchuk), Mikolaiv, 2011, pp. 112–115.
Strasburger, E., Uber Reduktionstheilung, Spindelbildung, Centrosome und Cilienbildner in Pflanzenreich, Hist. Beitr., 1900, vol. 6, pp. 1–284.
Twardovska, M.O., Andreev, I.O., and Kunakh, V.A., Kariotypes of species of the genus Iris L. of the flora of Ukraine, Ukr. Bot. Zh., 2014, vol. 71, no. 5, pp. 581–588.
Randolph, L.F. and Mitra, J., Karyotypes of Iris pumila and related species, Am. J. Bot., 1959, vol. 46, no. 2, pp. 93–102.
Mitra, J., Karyotype analysis of bearded iris, Ph.D. Thesis, Ithaca, NY: Cornell Univ., 1955.
Mitra, J., Karyotype analysis of bearded iris, Bot. Gaz., 1956, vol. 117, pp. 265–293.
Twarovska, M.O. and Kunakh, V.A., Introduction in in vitro culture of irises (Iris pumila L.), Introdukt. Roslin, 2013, no. 3, pp. 29–33.
Plokhinskii, N.A., Biometriya (Biometry), Moscow: Izd. MGU, 1970.
Kozyrenko, M.M., Artyukova, E.V., Lauve, L.S., and Boltenkov, E.V., Analysis of genetic stability of callus culture of some species of the genus Iris L., Biotekhnologiya, 2002, no. 4, pp. 38–48.
Cai, Z.Q., Zhang, T., and Jian, H.Y., Chromosome number variation in a promising oilseed woody crop, Plukenetia volubilis L. (Euphorbiaceae), Caryologia, 2013, vol. 66, no. 1, pp. 54–58.
Zhang, X.-H., Teixeira da Silva, J.A., and Ma, G.-H., Karyotype analysis of Santalum album L., Caryologia, 2010, vol. 63, no. 2, pp. 142–148.
Kunakh, V.A., Adonin, V.I., Ozheredov, S.P., and Blyum, Ya.B., Mixoploidy in wild and cultivated species of cruciferae capable of hybridizing with rapeseed Brassica napus, Cytol. Genet., 2008, vol. 42, no. 3, pp. 204–209.
Snowdon, R.J., Cytogenetics and genome analysis in Brassica crops, Chromosome Res., 2007, vol. 15, pp. 85–95.
Ockendon, D.J., The ploidy of plants obtained from anther culture of cauliflowers (Brassica oleracea var. botrytis), Ann. Appl. Biol., 2008, vol. 113, no. 2, pp. 319–325.
Bulgakov, V.P., Lauve, L.S., Chernoded, G.K., et al., Chromosome variation in ginseng cells transformed with the rolC plant oncogene, Russ. J. Genet., 2000, vol. 36, no. 2, pp. 150–156.
Su, C., Su, L.H., Zhu, G.Y., and Han, M.Z., The ways of bringing about and classifications of mixoploid in Morus L., North Sericult., 2000, vol. 21, pp. 11–12.
Joachimiak, A., Kula, A., Sliwinska, E., and Sobieszczanska, A., C-banding and nuclear DNA amount in six Bromus species, Acta Biol. Cracov. Ser. Bot., 2001, vol. 43, pp. 105–115.
Kunakh, V.A., Biotekhnologiya likars’kikh roslin. Genetichni ta fiziologo-biokhimichni osnovi (Biotechnology of Medicinal Plants: Genetic, Physiological, and Biochemical Basics), Kyiv: Logos, 2005.
Kunakh, V.A., Features of structural mutagenesis in populations of cultured plant cells, Usp. Sovrem. Genet., 1984, no. 12, pp. 30–62.
Hamrick, J.L. and Godt, M.J.W., Effects of life history traits on genetic diversity in plant species, Philos. Transact. Biol. Sci., 1996, vol. 351, no. 1345, pp. 1291–1298.
Simonet, M., Nouvelles recherches cytologiques et génétiques chez les Iris, Ann. Sci. Nat. Bot., 1934, vol. 16, pp. 229–383.
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Original Ukrainian Text © M.O. Twardovska, I.O. Andreev, V.A. Kunakh, 2015, published in Tsitologiya i Genetika, 2015, Vol. 49, No. 5, pp. 55–61.
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Twardovska, M.O., Andreev, I.O. & Kunakh, V.A. Intraspecific chromosomal polymorphism of Iris pumila L. from the territory of Ukraine. Cytol. Genet. 49, 322–327 (2015). https://doi.org/10.3103/S0095452715050096
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DOI: https://doi.org/10.3103/S0095452715050096