Skip to main content
SpringerLink
Log in

Molecular-genetic polymorphism of chs_H1 gene in Ukrainian hop varieties

  • Published:
Cytology and Genetics Aims and scope Submit manuscript

Abstract

Polymorphism of chs_H1 gene encoding the “true” chalcone synthase of hop was studied by alignment of nucleotide sequences in accordance with the Clustal W algorithm of the MEGA version 5 program and molecular-genetic analysis with the use of polymerase chain reaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lyashenko, I., Mikhailov, N.G., and Rudyk, R.I., Fiziologiya i biokhimiya khmelya (Physiology and biochemistry of hops), Zhitomir: Polissya, 2004.

    Google Scholar 

  2. Wang, G., Tian, L., Aziz, N., et al., Terpene biosynthesis in glandular trichomes of hop, Plant Physiol., 2008, vol. 148, no. 3, pp. 334–357.

    Google Scholar 

  3. Stevens, J.F., Taylor, A.W., Clawson, J.E., and Deinzer, M.L., Fate of xanthohumol and related prenylflavonoids from hops to beer, J. Agric. Food Chem., 1999, vol. 47, no. 6, pp. 2421–2428.

    Article  CAS  PubMed  Google Scholar 

  4. Christensen, A.B., Gregersen, P.L., Schroder, J., and Collinge, D.B., A chalcone synthase with an unusual substrate preference is expressed in barley leaves in response to UV light and pathogen attack, Plant. Mol. Biol., 1998, vol. 37, no. 5, pp. 849–857.

    Article  CAS  PubMed  Google Scholar 

  5. Nagel, J., Culley, L.K., Lu, Y., et al., EST analysis of hop glandular trichomes identifies an o-methyltransferase that catalyzes the biosynthesis of xanthohumol, Plant Cell, 2008, vol. 20, no. 1, pp. 186–200.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Ferrer, J., Jez, J.M., Bowman, M.E., et al., Structure of chalcone synthase and the molecular basis of plant polycetide biosynthesis, Nat. Struct. Biol., 1999, vol. 6, no. 8, pp. 775–784.

    Article  CAS  PubMed  Google Scholar 

  7. Durbin, M.L., McCaig, B., and Clegg, M.T., Molecular evolution of the chalcone synthase multigene family in the morning glory genome, Plant. Mol. Biol., 2000, vol. 42, no. 1, pp. 79–92.

    Article  CAS  PubMed  Google Scholar 

  8. Koes, R., Spelt, C., Elzen, P., and Mol, J., Cloning and molecular characterization of the chalcone synthase multigene family of petunia hybrida, Gene, 1989, vol. 81, no. 2, pp. 245–257.

    Article  CAS  PubMed  Google Scholar 

  9. Austin, M.B. and Noel, J.P., The chalcone synthase superfamily of type III polyketide synthases, Nat. Prod. Rep., 2003, vol. 20, no. 1, pp. 79–110.

    Article  CAS  PubMed  Google Scholar 

  10. Novak, P., Krofta, K., and Matousek, J., Chalcone synthase homologues from Humulus lupulus: some enzymatic properties and expression, Biol. Plant., 2006, vol. 50, no. 1, pp. 48–54.

    Article  CAS  Google Scholar 

  11. Okada, Y., Sano, Y., Kaneko, T., et al., Enzymatic reactions by five chalcone synthase homologs from hop (Humulus lupulus L.), Biosci. Biotechnol. Biochem., 2004, vol. 68, no. 5, pp. 1142–1145.

    Article  CAS  PubMed  Google Scholar 

  12. Shtan’ko, I.P., Shablikin, V.V., Mikhailichenko, K.P., et al., Catalogue of hops cultivars approved for distribution in Ukraine, Zhitomir: Polissya, 2010.

    Google Scholar 

  13. www.ncbi.nlm.nih.gov/

  14. Okada, Y., Saeki, K., Inaba, A., et al., Construction of gene expression system in hop (Humulus lupulus) lupulin gland using valerophenone synthase promoter, Plant Physiol., 2003, vol. 160, pp. 1101–1108.

    Article  CAS  Google Scholar 

  15. Technical manual. GenePrint STR Systems (Silver stain detection). Promega Corporation, 2001.

  16. Tamura, K., Peterson, D., Peterson, N., et al., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol., 2011, vol. 28, no. 10, pp. 2731–2739.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Matousek, J., Novak, P., Briza, J., et al., Cloning and characterisation of chs-specific DNA and cDNA sequences from hop (Humulus lupulus L.), Plant Sci., 2002, vol. 162, no. 6, pp. 1007–1018.

    Article  CAS  Google Scholar 

  18. Patzak J., Vrba L., and Matousek, J., New STS molecular markers for assessment of genetic diversity and DNA fingerprinting in hop (Humulus lupulus L.), Genome, 2007, vol. 50, no. 1, pp. 15–25.

    Article  CAS  PubMed  Google Scholar 

  19. Martin, C.R., Structure, function and regulation of the chalcone synthase, Int. Rev. Cytol., 1993, vol. 147, pp. 233–284.

    Article  CAS  PubMed  Google Scholar 

  20. Rushton, P.J. and Somssich, I.E., Transcriptional control of plant genes responsive to pathogens, Curr. Opin. Plant. Biol., 1998, vol. 1, no. 4, pp. 311–315.

    Article  CAS  PubMed  Google Scholar 

  21. Sivolap, Yu., Zakharova, O., Kozhukhova, N., et al., Modern biotechnology is useful in the estimation of the genetic diversity of Ukrainian varieties of hop (Humulus lupulus L.), Cytol. Genet., 2010, vol. 44, no. 5, pp. 263–271.

    Article  Google Scholar 

  22. Murakami, A., Structural differences in the intergenic spacer of 18S–26S rDNA and molecular phylogeny using partial external transcribed spacer sequence in hop, Humulus lupulus L., Breed. Sci., 2001, vol. 51, no. 3, pp. 163–170.

    Article  CAS  Google Scholar 

  23. Murakami, A., Darby, P., Jovornik, B., et al., Molecular phylogeny of wild hops, Humulus lupulus L., Heredity, 2006, vol. 97, no. 1, pp. 66–74.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant-Breeding and Genetics Institute, National Center of Seed and Cultivar Investigation, Odessa, Ukraine

    A. M. Venger, N. E. Volkova & Yu. M. Sivolap

Authors
  1. A. M. Venger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. E. Volkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu. M. Sivolap
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. E. Volkova.

Additional information

Original Ukrainian Text © A.M. Venger, N.E. Volkova, Yu.M. Sivolap, 2015, published in Tsitologiya i Genetika, 2015, Vol. 49, No. 5, pp. 20–25.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Venger, A.M., Volkova, N.E. & Sivolap, Y.M. Molecular-genetic polymorphism of chs_H1 gene in Ukrainian hop varieties. Cytol. Genet. 49, 294–298 (2015). https://doi.org/10.3103/S0095452715050102

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0095452715050102

Keywords

Search

Navigation