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Genetic variability of maternal plants and embryos of their seeds in the populations of Pinus kochiana Klotzsch ex Koch in Crimea

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Abstract

Samples of trees and seed embryos of five Pinus kochiana Klotzsch ex Koch populations in Crimea were comparatively studied at 12 allozymic loci for its genetic variability and mating system. It has been shown that the embryos of seeds reproduce the allelic diversity of maternal plants, but the level of their observed heterozygosity (Ho) is significantly lower, i.e., 0.286 vs. 0.189, respectively. Unlike maternal trees, embryos are characterized by a significant divergence between the observed distribution of genotypes and the theoretically expected one according to the Hardy–Weinberg law for the majority of analyzed loci. The frequency of cross pollination in populations varied from 0.384 to 0.673 according to single-locus (t s) estimates and from 0.639 to 0.841 according to multilocus (t m) estimates.

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References

  1. Altukhov, Y.P., The Dynamics of Population Gene Pools Under Anthropogenic Influences, Moscow: Nauka, 2004.

    Google Scholar 

  2. Didukh, Ya.P., The Vegetation Cover of the Crimean Mountains (Structure, Dynamics, and Evolution of the Security), Kyiv: Naukova dumka, 1992.

    Google Scholar 

  3. Korshikov, I.I., Podgornyi, D.Y., Kalafat, L.A., and Pirko, Ya.V., Taxonomic status of Pinus kochiana Klotzsch ex Koch from Mountain Crimea on data comparative population and genetic research to Pinus sylvestris L., Ukr. Bot. J., 2011, vol. 68, no. 1, pp. 133–142.

    Google Scholar 

  4. Delgado, P., Cuenca, A., Escalante, A.E., Molina-Freaner, F., and Pinero, D., Comparative genetic structure in pines: evolutionary and conservation consequences, Rev. Chil. Hist. Nat., 2002, vol. 75, no. 1, pp. 27–37.

    Article  Google Scholar 

  5. Politov, D.V., Genetics of populations and evolutionary relationships of pine species (Family Pinaceae) of northern Eurasia, Extended Abstract of Doctoral (Biol.) Dissertation, Moscow, 2007.

    Google Scholar 

  6. Davis, B.J., Disk electrophoresis. 2. Methods and application to human serum proteins, Ann. N.Y. Acad. Sci., 1964, vol. 121, pp. 404–427.

    Article  CAS  PubMed  Google Scholar 

  7. Nei, M., Genetic distance between populations, Am. Nat., 1972, vol. 106, no. 949, pp. 283–292.

    Article  Google Scholar 

  8. Swofford, D.L. and Selander, R.B., BIOSYS-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematic, J. Hered., 1981, vol. 72, no. 4, pp. 281–283.

    Google Scholar 

  9. Peakall, R. and Smouse, P.E., GenAlex 6: Genetic Analysis in Excel. Population genetic software for teaching and research, Mol. Ecol. Notes, 2006, vol. 6, no. 1, pp. 288–295.

    Article  Google Scholar 

  10. Ritland, K., Extensions of models for the estimation of mating systems using n independent loci, Heredity, 2002, vol. 88, pp. 221–228.

    Article  PubMed  Google Scholar 

  11. Bower, A.D. and Aitken, S.N., Mating system and inbreeding depression in whitebark pine (Pinus albicaulis Engelm.), Tree Genet. Genom., 2007, vol. 3, no. 4, pp. 379–388.

    Article  Google Scholar 

  12. Korshikov, I.I., Pirko, N.N., Mudrik, E.A., and Pirko, Ya.V., Maintenance of genetic structure in progenies of marginal mountainous and steppe populations of three species of Pinacea Lindl. Family in Ukraine, Silvae Genet., 2007, vol. 56, no. 1, pp. 1–10.

    Google Scholar 

  13. Belokon, M.M., Belokon, Yu.S., and Politov, D.V., The mating system and the dynamics of allozyme heterozygosity in Pinus pumila (Pall.) Regel. populations, Conifers Boreal Zone, 2010, vol. 27, nos. 1–2, pp. 13–18.

    Google Scholar 

  14. Ledig, F.T., Capo-Arteaga, M.A., Hodgskiss, P.D., Sbay, H., Flores-Lopez, C., Conkle, M.T., and Bermejo-Velazquez, B., Genetic diversity and the mating system of a rare Mexican pinon, Pinus pinceana, and a comparison with Pinus maximartinezii (Pinaceae), Am. J. Bot., 2001, vol. 88, no. 11, pp. 1977–1987.

    CAS  Google Scholar 

  15. Fady, B., Forest, I., Hochu, I., Ribiollet, A., de Beaulieu, J.-L., and Pastuszka, P., Genetic differentiation in Abies alba Mill. populations from southeastern France, Forest Genet., 1999, vol. 6, no. 3, pp. 129–138.

    Google Scholar 

  16. Ledig, F.T., Hodgskiss, P.D., and Johnson, D.R., Genic diversity, genetic structure, and mating system of Brewer spruce (Pinaceae), a relict of the Arcto-Tertiary forest, Am. J. Bot., 2005, vol. 92, no. 12, pp. 1975–1986.

    Google Scholar 

  17. Muona, O., Yazdani, R., and Rudin, D., Genetic change between life stages in Pinus sylvestris: allozyme variation in seeds and planted seedlings, Silvae Genet., 1987, vol. 36, no. 1, pp. 39–42.

    Google Scholar 

  18. Yazdani, R., Muona, O., Rudin, D., and Szmidt, A.E., Genetic structure of Pinus sylvestris L. seed-tree stand and naturally regenerated understorey, Forest Sci., 1985, vol. 31, no. 2, pp. 430–436.

    Google Scholar 

  19. Politov, D.V., Pirko, Y.V., Pirko, N.N., Mudrik, E.A., and Korshikov, I.I., Analysis of mating system in two Pinus cembra L. populations of the Ukrainian Carpathians, Ann. Forest Res., 2008, vol. 51, no. 1, pp. 11–18.

    Google Scholar 

  20. Restoux, G., Silva, D.E., Sagnard, F., Torre, F., Klein, E., and Fady, B., Life at the margin: the mating system of Mediterranean conifers, Web Ecol., 2008, vol. 8, pp. 94–102.

    Article  Google Scholar 

  21. Korshikov, I.I., Mudrik, E.A., and Terlyga, N.C., Analysis of genetic variability of seed embryos in trees characterized by different seed productivity in Crimean population of Pinus pallasiana D. Don, Tsitol. Genet., 2005, vol. 39, no. 2, pp. 27–33.

    CAS  PubMed  Google Scholar 

  22. Korshikov, I.I. and Mudrik, E.A., Comparative analysis of genetic heterogeneity of seed progenies in the isolated population of Pinus sylvestris var. cretacea Kalenicz ex Kom. in Donbass, Tsitol. Genet., 2006, vol. 40, no. 3, pp. 17–23.

    CAS  PubMed  Google Scholar 

  23. Korshikov, I.I. and Mudrik, E.A., Elevation-dependent genetic variation of plants and seed embryos in the Crimea mountain population of Pinus pallasiana D. Don, Russ. J. Ecol., 2006, vol. 37, no. 2, pp. 79–83.

    Article  CAS  Google Scholar 

  24. Privalikhin, S.N. and Makogon, I.V., Analysis of the mating system in two populations of Norway spruce (Picea abies (L.) Karst.) from Ukrainian Polissya, in The Science of Forest XXI Century: Proc. Int. Sci.-Pract. Conf., Gomel, 2010, pp. 268–269.

    Google Scholar 

  25. Altukhov, Yu.P., Genetic Processes in Populations, Moscow: Akademkniga, 2003.

    Google Scholar 

  26. Conservation and Management of Forest Genetic Resources in Europe, Geburek, Th. and Turok, J., Eds., Zvolen: Arbora Publ., 2005.

  27. Korshikov, I.I., Kalafat, L.A., Lisnichuk, A.N., Velikorid’ko, T.I., and Mudrik, E.A., Allozyme variation of seed embryos and mating system in relict populations of Scots pine (Pinus sylvestris L.) from the Kremenets hill ridge and Maloe Poles’e, Russ. J. Genet., 2011, vol. 47, no. 7, pp. 829–835.

    Article  CAS  Google Scholar 

  28. Hosius, B., Bergmann, F., Konnert, M., and Henkel, W., A concept for seed orchards based on isoenzyme gene markers, Forest Ecol. Manage, 2000, vol. 131, nos. 1–3, pp. 143–152.

    Article  Google Scholar 

  29. Bush, I.R. and Smouse, P.A., Evidence for the adaptive significance of allozymes in forest trees, New Forests, 1992, vol. 6, no. 1, pp. 179–196.

    Article  Google Scholar 

  30. Korshikov, I.I., Podgornyi, D.Yu., Kalafat, L.A., and Velikorid’ko, T.I., Population-and-genetic variation of the Pinus kochiana Klotzsch ex Koch in the Mountain Crimea, Rep. Natl. Acad. Sci. Ukraine, 2010, no. 5, pp. 161–167.

    Google Scholar 

  31. Korshikov, I.I., Podgorny, D.Yu., and Lisnichuk, A.N., Population and genetic differences between Koch pine (Pinus kochiana Klotzsch ex Koch) of mountain Crimea and Scotch pine (Pinus sylvestris L.) of Kremenets hill, Bul. Nikit. Botan. Sada, 2012, no. 104, pp. 22–26.

    Google Scholar 

  32. Korshikov, I.I., Velikorid’ko, T.I., Koba, V.P., Podgorny, D.Yu., Kalafat, L.A., and Gorlova, E.V., Genetic control of isozymes pine Koch in the mountain Crimea, Trudy Nikit. Bot. Sada, 2008, vol. 130, pp. 112–119.

    Google Scholar 

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Authors and Affiliations

  1. Kryvyi Rih Botanical Garden, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    I. I. Korshykov

  2. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    L. A. Kalafat

  3. Donetsk Botanical Garden, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    I. I. Korshykov, L. A. Kalafat & O. M. Vynogradova

  4. Nikita Botanical Garden, National Scientific Center, Ukrainian Academy of Agricultural Sciences, Kyiv, Ukraine

    D. Y. Podgornyi

Authors
  1. I. I. Korshykov
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  2. L. A. Kalafat
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  3. O. M. Vynogradova
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  4. D. Y. Podgornyi
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Correspondence to I. I. Korshykov.

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Original Ukrainian Text © I.I. Korshykov, L.A. Kalafat, O.M. Vynogradova, D.Y. Podgornyi, 2016, published in Tsitologiya i Genetika, 2016, Vol. 50, No. 2, pp. 36–43.

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Korshykov, I.I., Kalafat, L.A., Vynogradova, O.M. et al. Genetic variability of maternal plants and embryos of their seeds in the populations of Pinus kochiana Klotzsch ex Koch in Crimea. Cytol. Genet. 50, 110–115 (2016). https://doi.org/10.3103/S0095452716020079

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