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Polyphasic taxonomic analysis of Bacillus sp. strain C6—the antagonist of phytopathogenic microorganisms

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Polyphasic taxonomic analysis was carried out for Bacillus sp. strain C6, as the antagonist of phytopathogenic bacteria and micromycetes. The combination of cultural, morphological, physiological, and biochemical properties of the strain has enabled researchers to refer it to the Bacillus subtilis group. It has been shown that the fatty acids of the strain’s cell walls were predominantly represented by branched iso- and anteiso-C15:0 and C17:0 fatty acids (over 85%), which was typical for the Bacillus amyloliquefaciens species. The molecular genetic analysis carried out on the nucleotide sequence of the 16S rRNA gene, and the profiling of polymorphic nucleotides have enabled researchers to refer the strain in question to Bacillus amyloliquefaciens subsp. plantarum.

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References

  1. Prakash, O., Verma, M., Sharma, P., Kumar, M., Kumari, K., Singh, A., Kumari, H., Jit, S., Gupta, S.K., Khanna, M., and Lal, R., Polyphasic approach of bacterial classification—an overview of recent advances, J. Microbiol., 2007, vol. 47, no. 2, pp. 98–108.

    CAS  Google Scholar 

  2. Vandamme, P., Pot, B., Gillis, M., de Vos, P., Kersters, K., and Swings, J., Polyphasic taxonomy, a consensus approach to bacterial systematic, Microbiol. Rev., 1996, vol. 60, no. 2, pp. 407–438.

    CAS  PubMed  Google Scholar 

  3. Gevers, D., Cohan, F.M., Lawrence, J.G., Spratt, B.G., Coenye, T., Feil, E.J., Stackebrandt, E., de Peer, Y.V., Vandamme, P., Thompson, F.L., and Swings, J., Reevaluating prokaryotic species, Nature Rev. Microbiol., 2005, vol. 3, no. 9, pp. 733–739.

    Article  CAS  Google Scholar 

  4. Bernardet, J.F., Vancanneyt, M., Matte-Tailliez, O., Grisez, L., Tailliez, P., Bizet, C., Nowakowsk, M., Kerouault, B., and Swings, J., Polyphasic study of chryseobacterium strains isolated from diseased aquatic animals, Syst. Appl. Microbiol., 2005, vol. 28, no. 7, pp. 640–660.

    Article  CAS  PubMed  Google Scholar 

  5. Dahllöf, I., Molecular community analysis of microbial diversity, Curr. Opin. Biotechnol., 2002, vol. 13, no. 3, pp. 213–217.

    Article  PubMed  Google Scholar 

  6. Berkeley, R., Heyndrickx, M., Logan, N., and de Vos, P., Applications and Systematics of Bacillus and Relative, Wiley-Blackwell, 2002.

    Book  Google Scholar 

  7. Grabova, A.Yu., Dragovoz, I.V., Zelenaya, L.B., Kryuchkova, L.A., Pasichnik, L.A., and Avdeeva, L.V., Screening of strains of bacilli—antagonists of pathogenic microorganisms, in Materialy XIUkr. Biokhim. Kongr. (Proc. XI Ukr. Biochem. Congr.), Kyiv, 2014, p. 180.

    Google Scholar 

  8. Netrusov, A.I., Egorov, M.A., and Zakharchuk, L.M., Praktikum po mikrobiologii (A Practical Course in Microbiology), Moscow, 2005.

    Google Scholar 

  9. Smirnov, V.V., Reznik, S.R., and Sorokulova, I.B., Metodicheskie rekomendatsii po vydeleniyu i identifikatsii bakterii gruppy Bacillus subtilis-mesentericus iz organizma cheloveka i zhivotnykh (Guidelines for the Isolation and Identification of Bacteria of the group Bacillus subtilismesentericus of Humans and Animals), Kiev, 1980.

    Google Scholar 

  10. Reva, O.N., Smirnov, V.V., Pettersson, B., and Priest, F.G., Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.), Int. J. Syst. Evol. Microbiol., 2002, vol. 52, pp. 101–107.

    CAS  PubMed  Google Scholar 

  11. Bergey’s Manual of Systematic Bacteriology, Vol. 3: The Firmicutes, Vos, P., Garrity, G., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K.-H., and Whitman, W.B., Eds., New York: Springer, 2009.

  12. Lane, D.G., 16S/23S rRNA sequencing, in Nucleic Acids Techniques in Bacterial Systematic, Stackebrandt, E. and Goodfellow, M., Eds., Chichester, UK: Wiley, 1991, pp. 115–175.

    Google Scholar 

  13. Safronova, L.A., Zelena, L.B., Klochko, V.V., and Reva, O.N., Does the applicability of Bacillus strains in probiotics rely upon their taxonomy, Can. J. Microbiol., 2012, vol. 58, no. 2, pp. 212–219.

    Article  CAS  PubMed  Google Scholar 

  14. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol., 2011, vol. 28, no. 10, pp. 2731–2739.

    Article  CAS  PubMed  Google Scholar 

  15. Reva, O.N., Sorokulova, I.B., and Smirnov, V.V., Simplified technique for identification of the aerobic sporeforming bacteria by phenotype, Int. J. Syst. Evol. Microbiol., 2001, vol. 51, pp. 1361–1371.

    Article  CAS  PubMed  Google Scholar 

  16. Kaneda, T., Fatty acids of the genus Bacillus: an example of branched-chain preferences, Bacteriol. Rev., 1977, vol. 41, no. 2, pp. 391–418.

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Ehrhardt, C.J., Chu, V., Brown, T.C., Simmons, T.L., Swan, B.K., Bannan, J., and Robertson, J.M., Use of fatty acid methyl ester profiles for discrimination of Bacillus cereus T-strain spores grown on different media, Appl. Environ. Microbiol., 2010, vol. 76, no. 6, pp. 1902–1912.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Ibragimova, M.Y., Salafutdinov, I.I., Sahin, F., and Zhdanov, R.I., Biomarkers of Bacillus subtilis total lipids fame profile under various temperatures and growth phases, Dokl. Biochem. Biophys., 2012, vol. 443, no. 5, pp. 109–112.

    Article  CAS  PubMed  Google Scholar 

  19. Borriss, R., Chen, X.-H., Rueckert, C., Blom, J., Becker, A., Baumgarth, B., Fan, B., Pukall, R., Schumann, P., Sproer, C., Junge, H., Vater, J., Puhler, A., and Klenk, H.-P., Relationship of Bacillus amiloliquefaciens clades associated with strains DSM7T and FZB42T: a proposal for Bacillus amiloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amiloliquefaciens subsp. plantarum subsp. nov. based on their discriminating complete genome sequences comparisons, Int. J. Syst. Evol. Microbiol., 2011, vol. 61, pp. 1786–1801.

    Article  CAS  PubMed  Google Scholar 

  20. Ruiz-Garcia, N., Bejar, V., Martinez-Checa, F., Llamas, I., and Quesada, E., Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Velez in Malaga, southern Spain, Int. J. Syst. Evol. Microbiol., 2005, vol. 55, pp. 191–195.

    Article  CAS  PubMed  Google Scholar 

  21. Jeyaram, K., Romi, W., Singh, T.A., Adewumi, G.A., Basanti, K., and Oguntoyinbo, F.A., Distinct differentiation of closely related species of Bacillus subtilis group with industrial importance, J. Microbiol. Methods, 2011, vol. 87, no. 2, pp. 161–164.

    Article  CAS  PubMed  Google Scholar 

  22. Goto, K., Omura, T., Hara, Y., and Sadaie, Y., Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus, J. Gen. Appl. Microbiol., 2000, vol. 46, no. 1, pp. 1–8.

    Article  CAS  PubMed  Google Scholar 

  23. Idriss, E.E., Makarewicz, O., Farouk, A., Rosner, K., Greiner, R., Bochow, H., Richter, T., and Borriss, R., Extracellular phytase activity of Bacillus amyloliquefaciens FZB45 contributes to its plant-growth-promoting effect, Microbiology, 2002, vol. 148, pp. 2097–2109.

    Article  CAS  PubMed  Google Scholar 

  24. Reva, O.N., Dixelius, C., Meijer, J., and Priest, F.G., Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis, FEMS Microbiol. Ecol., 2004, vol. 48, no. 2, pp. 249–259.

    Article  CAS  PubMed  Google Scholar 

  25. Safronova, L.A., Zelenaya, L.B., Klochko, V.V., Avdeeva, L.V., Reva, O.N., and Podgorskii, V.S., Genotypic and phenotypic characterization of strains of bacilli that are endosporin components, Mikrobiol. Zh., 2012, vol. 74, no. 5, pp. 55–65.

    CAS  Google Scholar 

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Correspondence to G. Yu. Grabova.

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Original Ukrainian Text © G.Yu. Grabova, I.V. Dragovoz, L.B. Zelena, A.N. Ostapchuk, L.V. Avdeeva, 2016, published in Tsitologiya i Genetika, 2016, Vol. 50, No. 4, pp. 62–68.

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Grabova, G.Y., Dragovoz, I.V., Zelena, L.B. et al. Polyphasic taxonomic analysis of Bacillus sp. strain C6—the antagonist of phytopathogenic microorganisms. Cytol. Genet. 50, 251–256 (2016). https://doi.org/10.3103/S0095452716040046

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  • DOI: https://doi.org/10.3103/S0095452716040046

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