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Diversity of Ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes

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Diversity of Ukrainian winter common wheat varieties was studied with respect to the storage protein loci Gli-A1, Gli-B1, Gli-D1, Glu-A1, Glu-B1, Glu-D1, Gli-A3, Gli-B5, and Gli-A6 (362 varieties) and markers for the Lr34/Yr18/Pm38/Sr57/Bdv1 gene conferring moderate resistance to a number of biotrophic pathogens, the Tsn1 gene for sensitivity to the toxins A of the necrotrophic fungi Pyrenophora tritici-repentis and Stagonospora nodorum, the Tsc2 gene for sensitivity to the toxin B of P. tritici-repentis, and the TDF_076_2D gene for moderate resistance to Fusarium head blight (181 varieties). Significant differences in frequencies of alleles at these marker loci between groups of varieties developed in different soil and climatic zones were revealed. The retention of a set of predominant alleles in groups of varieties of a certain zone in different periods of breeding was confirmed. At the same time, the appearance of new allele associations in the groups of varieties of the Steppe (in particular Gli-A1g and Glu-B1al) and the Central Forest-Steppe (1AL/1RS and Glu-B1d) in the last two decades has been noted. Nonrandom associations between alleles of disease resistance genes as well as alleles of disease resistance genes and storage protein alleles were revealed.

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References

  1. Matsuoka, Y., Evolution of polyploid Triticum wheats under cultivation: the role of domestication, natural hybridization and allopolyploid speciation in their diversification, Plant Cell Physiol., 2011, vol. 52, no. 5, pp. 750–764.

    Article  CAS  PubMed  Google Scholar 

  2. Shewry, P.R., Wheat, J. Exp. Bot., 2009, vol. 60, no. 6, pp. 1537–1553.

    Article  CAS  PubMed  Google Scholar 

  3. Sozinov, A.A., and Poperelya, F.A., Genetic classification of prolamines and its use for plant breeding, Ann. Technol. Agric., 1980, vol. 29, pp. 229–245.

    CAS  Google Scholar 

  4. Payne, P.I., and Lawrence, G.J., Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat, Cereal Res. Commun., 1983, vol. 11, no. 1, pp. 29–35.

    Google Scholar 

  5. Sobko, T.A., and Poperelya, F.A., The frequency of alleles of gliadin-coding loci in different cultivars of winter common wheat, Visnyk Silskogospodarskoi Nauki, 1986, no. 5, pp. 84–87.

    Google Scholar 

  6. Chernakov, V.M., and Metakovsky, E.V., Diversity of gliadin-coding locus allelic variants and evaluation of genetic similarity of common wheat varieties from different breeding centers, Russ. J. Genet., 1994, vol. 30, no. 4, pp. 509–517.

    Google Scholar 

  7. Metakovsky, E.V., and Branlard, G., Genetic diversity of French common wheat germplasm based on gliadin alleles, Theor. Appl. Genet., 1998, vol. 96, no. 2, pp. 209–218.

    Article  CAS  Google Scholar 

  8. Sobko, T.A., and Sozinov, A.A., Analysis of genotypic structure of common wheat cultivars licensed for growing in Ukraine using genetic markers, Tsitol. Genet., 1999, vol. 33, no. 5, pp. 30–41.

    Google Scholar 

  9. Metakovsky, E.V., Gomes, M., Vasquez, J.F., and Carrillo, J.M., High genetic diversity of Spanish common wheats as judged from gliadin alleles, Plant Breed., 2000, vol. 119, no. 1, pp. 37–42.

    Article  CAS  Google Scholar 

  10. Novosel’skaya-Dragovich, A.Y., Krupnov, V.A., Saifulin, R.A., and Pukhalskiy, V.A., Dynamics of genetic variation at gliadin-coding loci in Saratov cultivars of common wheat Triticum aestivum L. over eight decades of scientific breeding, Russ. J. Genet., 2003, vol. 39, no. 10, pp. 1130–1137.

    Article  Google Scholar 

  11. Wrigley, C.W., Bekes, F., Cavanagh, C.R., and Bushuk, W., The Gluten Composition of Wheat Varieties and Genotypes, 2006, http://www.aaccnet.org/initiatives/definitions/ Pages/gliadin.aspx

    Google Scholar 

  12. Payne, P.I., Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality, Annu. Rev. Plant Physiol., 1987, vol. 38, pp. 141–153.

    Article  CAS  Google Scholar 

  13. Payne, P.I., Holt, L.M., Jackson, E.A., and Law, C.N., Wheat storage proteins: their genetics and their potential for manipulation by plant breeding, Phil. Trans. Roy. Soc. Lond. B, 1984, vol. 304, no. 1120, pp. 359–371.

    Article  CAS  Google Scholar 

  14. Oury, F.-X., Chiron, H., Faye, A., Gardet, O., Giraud, A., Heumez, E., Rolland, B., Rousset, M., Trottet, M., Charmet, G., and Branlard, G., The prediction of bread wheat quality: joint use of the phenotypic information brought by technological tests and the genetic information brought by HMW and LMW glutenin subunits, Euphytica, 2010, vol. 171, pp. 87–109.

    Article  Google Scholar 

  15. Metakovsky, E.V., Chernakov, V.M., Upelniek, V.P., Redaelli, R., Dardevet, M., Branlard, G., and Pogna, N.E., Recombination mapping of minor ω-gliadin-coding loci on chromosome 1A of common wheat: A revision, J. Genet. Breed., 1996, vol. 50, pp. 277–286.

    CAS  Google Scholar 

  16. Payne, P.I., Holt, L.M., and Lister, P.G., Gli-A3 and Gli-B3, two newly designated loci coding for omegatype gliadins and D subunits of glutenin, Proc. 7th Int. Wheat Genetics Symp., Cambridge, 1988, pp. 999–1002.

    Google Scholar 

  17. Pogna, N.E., Metakovsky, E.V., Redaelli, R., Raineri, F., and Dachkevitch, T., Recombination mapping of Gli-5, a new gliadin-coding locus on chromosomes 1A and 1B in common wheat, Theor. Appl. Genet., 1993, vol. 87, no. 1–2, pp. 113–121.

    CAS  PubMed  Google Scholar 

  18. Sandhu, D., Sidhu, D., and Gill, K.S., Identification of expressed sequence markers for a major gene-rich region of wheat chromosome group 1 using RNA fingerprinting-differential display, Crop Sci., 2002, vol. 42, no. 4, pp. 1285–1290.

    Article  CAS  Google Scholar 

  19. Dilbirligi, M., Erayman, M., Sandhu, D., Sidhu, D., and Gill, K.S., Identification of wheat chromosomal regions containing expressed resistance genes, Genetics, 2004, vol. 166, no. 1, pp. 461–481.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Jones, S.S., Dvorak, J., and Qualset, C.O., Linkage relations of Gli-D1, Rg2, and Lr21 on the short arm of chromosome 1D in wheat, Genome, 1990, vol. 33, no. 6, pp. 937–940.

    Article  CAS  Google Scholar 

  21. Czarnecki, E.M., and Lukow, O.M., Linkage of stem rust resistance gene Sr33 and the gliadin (Gli-D1) locus on chromosome 1DS, Genome, 1992, vol. 35, no. 4, pp. 565–568.

    Article  Google Scholar 

  22. Cox, T.S., Raupp, W.J., and Gill, B.S., Leaf rust-resistance genes Lr41, Lr42, and Lr43 transferred from Triticum tauschii to common wheat, Crop Sci., 1994, vol. 34, no. 2, pp. 339–343.

    Article  Google Scholar 

  23. Payne, P.I., Holt, L.M., Johnson, R., and Snape, J.W., Linkage mapping of four gene loci, Glu-B1, Gli-B1, Rg1 and Yr10 on chromosome 1B of bread wheat, Genetica Agraria, 1986, vol. 40, pp. 231–242.

    CAS  Google Scholar 

  24. Sourdille, P., Robe, P., Tixier, M.-H., Doussinault, G., Pavoine, M.-T., and Bernard, M., Location of Pm3g, a powdery mildew resistance allele in wheat, by using a monosomic analysis and by identifying associated molecular markers, Euphytica, 1999, vol. 110, no. 3, pp. 193–198.

    Article  CAS  Google Scholar 

  25. Howes, N.K., Linkage between the Lr10 gene conditioning resistance to leaf rust, two endosperm proteins, and hairy glumes in hexaploid wheat, Can. J. Genet. Cytol., 1986, vol. 28, no. 4, pp. 595–600.

    Article  CAS  Google Scholar 

  26. Rabinovich, S.V., Importance of wheat-rye translocations for breeding modern cultivars of Triticum aestivum L., Euphytica, 1998, vol. 100, no. 1, pp. 323–340.

    Article  Google Scholar 

  27. Catalogue of Gene Symbols. Gene Catalogue, 2013 http: www.shigen.nig.ac.jp/wheat/komugi/genes/download.jspMacGene.

  28. Peng, J., Wang, H., Haley, S.D., Peairs, F.B., and Lapitan, N.L.V., Molecular mapping of the Russian wheat aphid resistance gene Dn2414 in wheat, Crop Sci., 2007, vol. 47, no. 6, pp. 2418–2429.

    Article  CAS  Google Scholar 

  29. Singh, R.P., Hodson, D.P., Jin Y., Lagudah, E.S., Ayliffe M.A., Bhavani, S., Rouse, M.N., Pretorius, Z.A., Szabo, L.J., Huerta-Espino, J., Basnet, B.R., Lan, C., and Hovmöller, M.S., Emergence and spread of new races of wheat stem rust fungus: Continued threat to food security and prospects of genetic control, Phytopathology, 2015, vol. 105, no. 7, pp. 872–884.

    Article  PubMed  Google Scholar 

  30. Krattinger, S.G., Lagudah, E.S., Spielmeyer, W., Singh, R.P., Huerta-Espino, J., McFadden, H., Bossolini, E., Selter, L.L., Keller, B., A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat, Science, 2009, vol. 323, no. 5919, pp. 1360–1363.

    Article  CAS  PubMed  Google Scholar 

  31. Lagudah, E.S., Krattinger, S.G., Herrera-Foessel, S., Singh, R.P., Huerta-Espino, J., Spielmeyer, W., Brown-Guedira, G., Selter, L.L., and Keller, B., Genespecific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens, Theor. Appl. Genet, 2009, vol. 119, no. 5, pp. 889–898.

    Article  CAS  PubMed  Google Scholar 

  32. Dakouri, A., McCallum, B.D., Walichnowski, A.Z., and Cloutier, S., Fine-mapping of the leaf rust Lr34 locus in Triticum aestivum (L.) and characterization of large germplasm collections support the ABC transporter as essential for gene function, Theor. Appl. Genet., 2010, vol. 121, no. 2, pp. 373–384.

    Article  CAS  PubMed  Google Scholar 

  33. Faris, J.D., Zhang, Z., Lu, H., Lu, S., Reddy, L., Cloutier, S., Fellers, J.P., Meinhardt, S.W., Rasmussen, J.B., Xu, S.S., Oliver, R.P., Simons, K.J., and Friesen, T.L., A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens, Proc. Natl. Acad. Sci. U. S. A., 2010, vol. 107, no. 30, pp. 13544–13549.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Diethelm, M., Schmolke, M., Groth, J., Friedt, W., Schweizer, G., and Hartl, L., Association of allelic variation in two NPR1-like genes with Fusarium head blight resistance in wheat, Mol. Breed., 2014, vol. 34, no. 1, pp. 31–43.

    Article  CAS  Google Scholar 

  35. Abeysekara, N.S., Friesen, T.L., Liu, Z., McClean, P.E., and Faris, J.D., Marker development and saturation mapping of the Tan Spot Ptr ToxB sensitivity locus Tsc2 in hexaploid wheat, Plant Genome, 2010, vol. 3, no. 3, pp. 179–189.

    Article  CAS  Google Scholar 

  36. Sozinov, A., Sozinov, I., Kozub, N., and Sobko, T., Stable gene associations in breeding and evolution of grasses, in Evolutionary Theory and Processes: Modern Perspectives, Wasser, S.P., Ed., Kluwer Acad. Publ., 1999, pp. 97–113.

    Chapter  Google Scholar 

  37. Blagodarova, O.M., Lytvynenko, M.A., and Golub, Ye.A., Gene geography of alleles at gliadin-and glutenin-coding loci of Ukrainian winter common wheat varieties and their association with agronomical traits, Collection of Scientific Papers of the Institute of Breeding and Genetics, 2004, vol. 46, no. 6, pp. 124–138.

    Google Scholar 

  38. Kozub, N.A., Sozinov, I.A., Sobko, T.A., Kolyuchii, V.T., Kuptsov, S.V., and Sozinov, A.A., Variation at storage protein loci in winter common wheat cultivars of the Central Forest-Steppe of Ukraine, Cytol. Genet., 2009, vol. 43, no. 1, pp. 55–62.

    Article  Google Scholar 

  39. Zaika, Ie.V., Kozub, N.A., Sozinov, I.A., Sozinov, A.A., and Starichenko, V.N., Analysis of genotypes of winter common wheat varieties of the Institute of Agriculture NAAS with respect to storage protein loci, Bull. Belar. Agric. Acad., 2014, no. 4, pp. 53–57.

    Google Scholar 

  40. Chebotar, S.V., and Sivolap, Yu.M., Differentiation, identification and development of database of T. aestivum L. varieties of Ukrainian selection on the basis of STMS-analysis, Tsitol. Genet., 2001, vol. 35, no. 6, pp. 18–27.

    CAS  PubMed  Google Scholar 

  41. Chebotar, S.V., Korzun, V.N., and Sivolap, Yu.M., Allele distribution at locus WMS261 marking the dwarfing gene Rht8 in common wheat cultivars of Southern Ukraine, Russ. J. Genet., 2001, vol. 37, no. 8, pp. 894–898.

    Article  CAS  Google Scholar 

  42. Petrova, I.V., Chebotar, S.V., Rybalka, A.I., and Sivolap, Yu.M., Identification of Wx genotypes in the winter wheat varieties, Cytol. Genet., 2007, vol. 41, no. 6, pp. 11–17.

    Article  CAS  Google Scholar 

  43. Chebotar, S.V., Börner, A., and Sivolap, Yu.M., Analysis of the dwarfing genes in the genotypes of bread wheat cultivars of Ukraine, Cytol. Genet., 2006, vol. 40, no. 4, pp. 12–23.

    CAS  Google Scholar 

  44. Karelov, A.V., Pirko, Ya.V., Kozub, N.A., Sozinov, I.A., Pirko, N.N., Litvinenko, N.A., Lyfenko, S.F., Koliuchiy, V.T., Blume, Ya.B., and Sozinov, A.A., Identification of allelic state of leaf rust-resistance Lr34 gene in the cultivars of bread winter wheat of Ukrainian breeding, Cytol. Genet., 2011, vol. 45, no. 5, pp. 271–276.

    Article  Google Scholar 

  45. Karelov, A.V., Kozub, N.A., Sozinov, I.A., Sozinov, A.A., and Blume, Ya.B., Allelic state of the moleculargenetic markers of the Tsn1 gene associated with Pyrenophora tritici-repentis and Stagonospora nodorum toxin A susceptibility among Ukrainian common wheat (Triticum aestivum L.) cultivars, Plant Protection and Quarantine (Zakhyst i Karantyn Roslyn), 2014, no. 60, pp. 106–113.

    Google Scholar 

  46. Galaev, A.V., and Sivolap, Yu.M., Description of the bread wheat varieties of Ukrainian and Russian breeding by alleles of locus csLV34 closely linked with multipathogen resistance gene Lr34/Yr18/Pm38, Cytol. Genet., 2015, vol. 49, no. 1, pp. 12–19.

    Article  Google Scholar 

  47. Karelov, A.V., Kozub, N.A., Sozinov, I.A., Borzykh O.I., and Blume, Ya.B., Polymorphism of the marker for the moderate Fusarium head blight resistance gene TDF_076_2D among common wheat (Triticum aestivum L.) varieties of the Steppe zone of Ukraine, Naukovi Dopovidi NUBiP Ukrainy, 2015, no. 2 (51).

    Google Scholar 

  48. Zaika, Ie.V., Karelov, A.V., Kozub, N.O., Sozinov, I.O., Sozinov, O.O., Starychenko, V.M., Analysis of Ukrainian Polissya and Forest-Steppe winter wheat (Triticum aestivum L.) cultivars for the presence of “resistant” allelic state of non-race-specific disease resistance locus Lr34/Yr18/Pm38, Ekin J. Crop Breed. Genet., 2015, vol. 1, no. 1, pp. 13–16.

    Google Scholar 

  49. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, no. 5259, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  50. Metakovsky, E.V., Gliadin allele identification in common wheat. II Catalogue of gliadin alleles in common wheat, J. Genet. Breed., 1991, vol. 45, pp. 325–344.

    Google Scholar 

  51. Clark-Carter, D., Doing Quantitative Psychological Research: From Design to Report, Hove, UK, Psychol. Press, 1997.

    Google Scholar 

  52. Nei, M., Analysis of gene diversity in subdivided populations, Proc. Natl. Acad. Sci. U. S. A., 1973, vol. 70, no. 12, pp. 3321–3323.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Hartl, D., Clark, A., Principles of Population Genetics, Sinauer, Sunderland, MA, 1997.

    Google Scholar 

  54. Perrier, X., and Jacquemoud-Collet, J.P., DARwin Software, 2006, http://darwin.cirad.fr/

    Google Scholar 

  55. Shan, X., Clayshulte, S.R., Haley, S.D., and Byrne, P.F., Variation for glutenin and waxy alleles in the UShard winter wheat germplasm, J. Cereal Sci., 2007, vol. 45, no. 2, pp. 199–208.

    Article  CAS  Google Scholar 

  56. Lerner, S.E., Kolman, M.A., and Rogers, W.J., Quality and endosperm storage protein variation in Argentinian grown bread wheat. 1. Allelic diversity and discrimination between cultivars, J. Cereal Sci., 2009, vol. 49, no. 3, pp. 337–345.

    Article  CAS  Google Scholar 

  57. Poperelya, F.O., and Blagodarova, O.M., Genetics of grain quality of first Ukrainian genotypes of superstrong wheat, Tsitol. Genet., 1998, vol. 32, no. 6, pp. 11–19.

    Google Scholar 

  58. Kozub, N.O., Sozinov, I.O., Koluchiy, V.T., Vlasenko, V.A., Sobko, T.O., and Sozinov, O.O., Identification of 1AL/1RS translocation in winter common wheat varieties of Ukrainian breeding, Tsitol. Genet., 2005, vol. 39, no. 4, pp. 20–24.

    CAS  PubMed  Google Scholar 

  59. Glazebrook, J., Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens, Annu. Rev. Phytopathol., 2005, vol. 43, pp. 205–227.

    Article  CAS  PubMed  Google Scholar 

  60. Brauc, S., De Vooght, E., Claeys, M., Geuns, J.M., Höfte, M., and Angenon, G., Overexpression of arginase in Arabidopsis thaliana influences defense responses against Botrytis cinerea, Plant Biol. (Stuttg), 2012, vol. 14, no. S1, pp. 39–45.

    Article  CAS  Google Scholar 

  61. Bolton, M.D., Kolmer, J.A., Xu, W.W., and Garvin, D.F., Lr34-mediated leaf rust resistance in wheat: transcript profiling reveals a high energetic demand supported by transient recruitment of multiple metabolic pathways, Mol. Plant Microbe Interact., 2008, vol. 21, no. 12, pp. 1515–1527.

    Article  CAS  PubMed  Google Scholar 

  62. Kollers, S., Rodemann, B., Ling, J., Korzun, V., Ebmeyer, E., Argillier, O., Hinze, M., Plieske, J., Kulosa, D., Ganal, M.W., and Röder, M.S., Whole genome association mapping of Fusarium head blight resistance in European winter wheat (Triticum aestivum L.), PLoS One, 2013, vol. 8, no. 2, p. e57500.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Institute of Plant Protection, NAAS of Ukraine, Kyiv, Ukraine

    N. A. Kozub, I. A. Sozinov & A. V. Karelov

  2. Institute of Food Biotechnology and Genomics, NAS of Ukraine, Kyiv, Ukraine

    N. A. Kozub, A. V. Karelov, Ya. B. Blume & A. A. Sozinov

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  1. N. A. Kozub
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Original Ukrainian Text © N.A. Kozub, I.A. Sozinov, A.V. Karelov, Ya.B. Blume, A.A. Sozinov, 2017, published in Tsitologiya i Genetika, 2017, Vol. 51, No. 2, pp. 53–67.

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Kozub, N.A., Sozinov, I.A., Karelov, A.V. et al. Diversity of Ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes. Cytol. Genet. 51, 117–129 (2017). https://doi.org/10.3103/S0095452717020050

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