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Formation of adaptive reactions in Arabidopsis thaliana wild-type and mutant jin1 plants under action of abscisic acid and salt stress

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Abstract

To elucidate the possible role of the transcription factor (TRF) JIN1/MYC2 in implementation of stress-protective effects of abscisic acid (ABA), the effect of exogenous ABA on the state of stomata and the activity of antioxidant enzymes and proline content under salt stress conditions in Arabidopsis thaliana plants of wild type (Col-0) and jin1 mutants with impaired jasmonate signaling was investigated. Treatment of leaves’ epidermis with ABA (10 or 100 μM) caused the closing of stomata in Col-0 plants but has hardly any influence on stomatal aperture in jin1 mutants. Salt stress (200 mM NaCl exposure for 24 h) caused a reduction of the water content in the plant leaves of both genotypes. Addition of 10 μM ABA into the growing medium contributed to a maintaining of normal hydration in wild-type but not in jin1 plants under salt stress. ABA treatment caused an almost twofold increase in proline content in the leaves of plants of both genotypes under normal conditions. Pretreatment with phytohormone contributed to enhancing the proline content in wild-type plants at salt stress and had a less significant effect on its amount in jin1 plants. Treatment with ABA under physiologically normal conditions increased the catalase activity in wild-type plants. Both genotypes under ABA influence showed increased activity of superoxide dismutase (SOD). Under salt stress conditions, higher activity of SOD, catalase, and guaiacol peroxidase was observed in ABA-treated wild-type plants but not in jin1 mutants. A conclusion about the participation of TRF JIN1/MYC2 in the formation of certain ABA-induced physiological responses of Arabidopsis plants was made.

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References

  1. Dombrecht, B., Xue, G.P., Sprague, S.J., Kirke-gaard, J.A., Ross, J.J., Reid, J.B., Fitt, G.P., Sewe-lam, N., Schenk, P.M., Manners, J.M., and Kazan, K., MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis, Plant Cell, 2007, vol. 19, no. 7, pp. 2225–2245.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Guo, J., Pang, Q., Wang, L., Yu, P., Li, N., and Yan, X., Proteomic identification of MYC2-dependent jasmonateregulated proteins in Arabidopsis thaliana, Proteome Sci., 2012, vol. 10, no. 1, p. 57. doi 10.1186/1477-5956-10-57

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Yadav, V., Mallappa, C., Gangappa, S.N., Bhatia, S., and Chattopadhyay, S., A basic helix-loop-helix transcription factor in Arabidopsis, MYC2, acts as a repressor of blue light-mediated photomorphogenic growth, Plant Cell, 2005, vol. 17, no. 7, pp. 1953–1966.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Palmieri, M.C., Sell, S., Huang, X., Scherf, M., Werner, T., Durner, J., and Lindermayr, C., Nitric oxideresponsive genes and promoters in Arabidopsis thaliana: a bioinformatics approach, J. Exp. Bot., 2008, vol. 59, no. 2, pp. 177–186.

    Article  CAS  PubMed  Google Scholar 

  5. Yastreb, T.O., Kolupaev, Yu.E., Shvidenko, N.V., Lugovaya, A.A., and Dmitriev A.P., Salt stress response in Arabidopsis thaliana plants with defective jasmonate signaling, Appl. Biochem. Microbiol., 2015, vol. 51, no. 4, pp. 451–454.

    Article  CAS  Google Scholar 

  6. Yastreb, T.O., Kolupaev, Yu.E., Lugovaya A.A., and Dmitriev, A.P., Content of osmolytes and flavonoids under salt stress in Arabidopsis thaliana plants defective in jasmonate signaling, Appl. Biochem. Microbiol., 2016, vol. 52, no. 2, pp. 210–215.

    Article  CAS  Google Scholar 

  7. Yastreb, T.O., Kolupaev, Yu.E., Karpets, Yu.V., and Dmitriev, A.P., Effect of nitric oxide donor on salt resistance of Arabidopsis jin1 mutants and wild-type plants, Russ. J. Plant Physiol., 2017, vol. 64, no. 2, pp. 207–214.

    Article  CAS  Google Scholar 

  8. Ton, J., Flors, V., and Mauch-Mani, B., The multifaceted role of ABA in disease resistance, Trends Plant Sci., 2009, vol. 14, no. 6, pp. 310–317.

    Article  CAS  PubMed  Google Scholar 

  9. Lorenzo, O., Chico, J.M., Sanchez-Serrano, J.J., and Solano, R., Jasmonate-insensitive1 encodes a MYC transcription factor essential to discriminate between different jasmonate-regulated defense responses in Arabidopsis, Plant Cell, 2004, vol. 16, no. 7, pp. 1938–1950.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Abe, H., Urao, T., Ito, T., Seki, M., Shinozaki, K., and Yamaguchi-Shinozaki, K., Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling, Plant Cell, 2003, vol. 15, no. 1, pp. 63–78.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Moons, A., Bauw, G., and Prinsen, E., Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant indica rice varieties, Plant Physiol., 1995, vol. 107, no. 1, pp. 177–186.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Nilson, S.E., and Assmann, S.M., The control of transpiration. insights from Arabidopsis, Plant Physiol., 2007, vol. 143, no. 1, pp. 19–27.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Akhiyarova, G.R., Fricke, W., Veselov, D.S., Kudoyarova G.R., and Veselov, S.Yu., ABA accumulation and distribution during the leaf tissues shows its role stomatal conductance regulation under short-term salinity, Tsitologiya, 2006, vol. 48, no. 11, pp. 918–923.

    CAS  Google Scholar 

  14. Hare, P.D., Cress, W.A., and van Staden, J., Proline synthesis and degradation: a model system for elucidating stress-related signal transduction, J. Exp. Bot., 1999, vol. 50, no. 333, pp. 413–434.

    CAS  Google Scholar 

  15. Liang, X., Zhang, L., Natarajan, S.K., and Becker, D.F., Proline mechanisms of stress survival, Antioxid. Redox Signal., 2013, vol. 19, no. 9, pp. 998–1011.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Zhou, B., and Guo, Z., Calcium is involved in the abscisic acid-induced ascorbate peroxidase, superoxide dismutase and chilling resistance in Stylosanthes guianensis, Biol. Plant, 2009, vol. 53, no. 1, pp. 63–68.

    Article  CAS  Google Scholar 

  17. Guajardo, E., Juan, A.C., and Contreras-Porcia, L., Role of abscisic acid (ABA) in activating antioxidant tolerance responses to desiccation stress in intertidal seaweed species, Planta, 2016, vol. 243, no. 3, pp. 767–781.

    Article  CAS  PubMed  Google Scholar 

  18. Novikova, G.V., Stepanchenko, N.S., Nosov, A.V., and Moshkov, I.E., At the beginning of the route: ABA perception and signal transduction in plants, Russ. J. Plant Physiol., 2009, vol. 56, no. 6, pp. 727–741.

    Article  CAS  Google Scholar 

  19. Gibeaut, D.M., Hulett, J., Cramer, G.R., and Seemann, J.R., Maximal biomass of Arabidopsis thaliana using a simple, low-maintenance hydroponic method and favorable environmental conditions, Plant Physiol., 1997, vol. 115, no. 2, pp. 317–319.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Ramírez, V., Coego, A., Lopez, A., Agorio, A., Flors, V., and Vera P., Drought tolerance in Arabidopsis is controlled by the OCP3 disease resistance regulator, Plant J., 2009, vol. 58, no. 4, pp. 578–591.

    Article  PubMed  Google Scholar 

  21. Iakovenko, O.M., Kretynin, S.V., Kabachevskaya, E.M., Lyakhnovich, G.V., Volotovski, D.I., and Kravets, V.S., Role of phospholipase C in ABA regulation of stomata function, Ukr. Bot. J., 2008, vol. 65, no. 4, pp. 605–613.

    Google Scholar 

  22. Savouré, A., Hua, X.J., Bertauche, N., Van Montagu, M., and Verbruggen, N., Abscisic acid-independent and abscisic acid-dependent regulation of proline biosynthesis following cold and osmotic stresses in Arabidopsis thaliana, Mol. Gen. Genet., 1997, vol. 254, no. 1, pp. 104–109.

    Article  PubMed  Google Scholar 

  23. Bates, L.S., Waldren, R.P., and Teare, I.D., Rapid determination of free proline for water stress studies, Plant Soil, 1973, vol. 39, no. 1, pp. 205–207.

    Article  CAS  Google Scholar 

  24. Karpets, Yu.V., Kolupaev, Yu.E., Lugovaya, A.A., and Oboznyi, A.I., Effect of jasmonic acid on the pro-/antioxidant system of wheat coleoptiles as related to hyperthermia tolerance, Russ. J. Plant Physiol., 2014, vol. 61, no. 3, pp. 339–346.

    Article  CAS  Google Scholar 

  25. Alscher, R.G., Erturk, N., and Heath, L.S., Role of superoxide dismutases (SODs) in controlling oxidative stress in plants, J. Exp. Bot., 2002, vol. 53, no. 372, pp. 1331–1341.

    Article  CAS  PubMed  Google Scholar 

  26. Geng, S., Misra, B.B., de Armas, E., Huhman, D.V., Alborn, H.T., Sumner, L.W., and Chen, S., Jasmonate-mediated stomatal closure under elevated CO2 revealed by time-resolved metabolomics, Plant J., 2016, vol. 88, no. 6, pp. 947–962.

    Article  CAS  PubMed  Google Scholar 

  27. Sánchez-Romera, B., Ruiz-Lozano, J.M., Li, G., Luu, D.T., Martinez-Ballesta, C.M., Carvajal, M., Zamarreno, A.M., García-Mina, J.M., Maurel, C., and Aroca, R., Enhancement of root hydraulic conductivity by methyl jasmonate and the role of calcium and abscisic acid in this process, Plant Cell Environ., 2014, vol. 37, no. 4, pp. 995–1008.

    Article  PubMed  Google Scholar 

  28. Kazan, K., Diverse roles of jasmonates and ethylene in abiotic stress tolerance, Trends Plant Sci., 2015, vol. 20, no. 4, pp. 219–229.

    Article  CAS  PubMed  Google Scholar 

  29. Kavi Kishor, P.B., Sangam, S., Amrutha, R.N., Sri Laxmi, P., Naidu, K.R., Rao, K.R.S.S., Rao, S., Reddy, K.J., Theriappan, P., and Sreenivasulu, N., Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance, Curr. Sci., 2005, vol. 88, no. 3, pp. 424–438.

    Google Scholar 

  30. Hu, X., Jiang, M., Zhang, J., Zhang, A., Lin, F., and Tan, M., Calcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H2O2 production in leaves of maize (Zea mays) plants, New Phytol., 2007, vol. 173, no. 1, pp. 27–38.

    Article  CAS  Google Scholar 

  31. Lama, R., Jaishee, N., and Chakraborty, U., Ameliorative effects of ABA and proline during drought stress in maize enhancing its protective mechanism, World Appl. Sci. J., 2016, vol. 34, no. 2, pp. 174–181.

    CAS  Google Scholar 

  32. Huang, X., Stettmaier, K., Michel, C, Hutzler, P., Mueller, M.J., and Durner, J., Nitric oxide is induced by wounding and influences jasmonic acid signaling in Arabidopsis thaliana, Planta, 2004, vol. 218, no. 6, pp. 938–946.

    Article  CAS  PubMed  Google Scholar 

  33. Lackman, P., González-Guzmán, M., Tilleman, S., Carqueijeiro, I., Pérez, A.C., Moses, T., Seo, M., Kanno, Y., Häkkinen, S.T., Montagu, M.C.E.V., Thevelein, J.M., Maaheimo, H., Oksman-Caldentey, K.M., Rodriguez, P.L., Rischer, H., and Goossens A., Jasmonate signaling involves the abscisic acid receptor PYL4 to regulate metabolic reprogramming in Arabidopsis and tobacco, Proc. Natl. Acad. Sci. U. S. A., 2011, vol. 108, no. 14, pp. 5891–5896.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Dokuchaev Kharkiv National Agrarian University, Kharkiv, 62483, Ukraine

    T. O. Yastreb, Yu. E. Kolupaev & A. A. Lugovaya

  2. Karazin Kharkiv National University, Kharkiv, 61077, Ukraine

    Yu. E. Kolupaev

  3. Institute of Cell Biology and Genetic Engineering, Ukraine National Academy of Sciences, Kyiv, 03143, Ukraine

    A. P. Dmitriev

Authors
  1. T. O. Yastreb
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  2. Yu. E. Kolupaev
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  3. A. A. Lugovaya
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  4. A. P. Dmitriev
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Corresponding author

Correspondence to T. O. Yastreb.

Additional information

Original Russian Text © T.O. Yastreb, Yu.E. Kolupaev, A.A. Lugovaya, A.P. Dmitriev, 2017, published in Tsitologiya i Genetika, 2017, Vol. 51, No. 5, pp. 3–11.

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Yastreb, T.O., Kolupaev, Y.E., Lugovaya, A.A. et al. Formation of adaptive reactions in Arabidopsis thaliana wild-type and mutant jin1 plants under action of abscisic acid and salt stress. Cytol. Genet. 51, 325–330 (2017). https://doi.org/10.3103/S0095452717050115

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  • DOI: https://doi.org/10.3103/S0095452717050115

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