Abstract
Tragopogon graminifolius DC. is a medicinal plant species of the genus Tragopogon L. (Asteraceae) that grows in different regions of Iran and is extensively used by locals. There is no report on genetic variability and population structure of this important plant species. Therefore, we studied the genetic diversity, population structure and morphological variability of 14 geographical populations of Tragopogon graminifolius in Iran. AMOVA and Gst analyses revealed significant molecular differences among the studied populations. Mantel test showed significant correlation between genetic distance and geographical distance of the studied populations and indicated that the neighboring populations had a higher degree of gene flow. STRUCTURE plot identified three main gene pools for Tragopogon graminifolius in Iran and population assignment test revealed that gene flow occurred mostly among populations of the same gene flow. The studied populations differed significantly in their morphological and genetic features. These results may be of use for future conservation of this important plant species.
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References
Bremer, K., Asteraceae. Cladistics and Classification, Portland: Timber Press, 1994.
Blanca, G. and Diaz de la Guardia, C., Fruit morphology in Tragopogon L. (Compositae:Lactuceae) from the Iberian peninsula, Bot. J. Linn. Soc., 1997, vol. 125, no. 4, pp. 319–329.
Rechinger, K.H., Tragopogon, Flora Iranica, Compositae II. Lactuceae, Rechinger, K.H., Ed., Graz. Akad. Drucku Verlagsanstalt, 1977, pp. 46–83.
Awe, E.O., Adeloye, A., Idowu, T., Olajide, O.A., and Makinde, J., Antinociceptive effect of Russelia equisetiformis leave extracts: identification of its active constituents, Phytomedicine, 2008, vol. 15, no. 4, pp. 301–305.
Barnea, G., Barbara, A., Paula, E., and Bannister, J.P., Plant Growth Regulation, New York: Springer, 1989.
Farzaei, M.H., Khazaei, M., Abasabadi, Z., Feyzmahdavi, M., and Mohseni, G.R., Protective effect of Tragopogon graminifolius DC. against ethanol induced gastric ulcer in rat, Iran. Red Crescent Med. J., 2013, vol. 15, pp. 813–816.
Sheidai, M., Zanganeh, S., Haji-Ramezanali, R., Nouroozi, M., Noormohammadi, Z., and Ghsemzadeh- Baraki, S., Genetic diversity and population structure in four Cirsium (Asteraceae) species, Biologia, 2013, vol. 68, no. 3, pp. 384–397.
Azizi, N., Sheidai, M., Mozafarian, V., and Noormohammadi, Z., Genetic, cytogenetic and morphological diversity in Helicrysum leucocephalum (Asteraceae) populations, Biologia, 2014, vol. 69, no. 5, pp. 566–573.
Chen, X.Y., Effects of habital fragmentation on genetic structure of plant populations and implications for the biodiversity conservation, Acta Ecol. Sin., 2000, vol. 20, no. 5, pp. 884–892.
Ellis, J.R. and Burke, J.M., Est-ssrs as a resource for population genetic analyses, Heredity, 2007, vol. 99, no. 2, pp. 125–132.
Taban, M., Sheidai, M., Noormohammadi, Z., Azizi, N., Ghasemzadeh-Baraki, S., and Koohdar, F., Population genetic analysis and evidence of inter-specific introgression in Helichrysum armenium and H. rubicundum (Asteraceae), Genetika, 2015, vol. 47, no. 2, pp. 451–468.
Cassel-Lundhagen, A., Tammaru, T., Windig, J.J., Ryrholm, N., and Nylin, S., Are peripheral populations special? Congruent patterns in two butterfly species, Ecography, 2009, vol. 32, no. 4, pp. 591–600.
Sheidai, M., Seif, E., Nouroozi, M., and Noormohammadi, Z., Cytogenetic and molecular diversity of Cirsium arvense (Asteraceae) populations in Iran, J. Japan. Bot., 2012, vol. 87, pp. 193–205.
Sheidai, M., Ziaee, S., Farahani, F., Talebi, S.M., Noormohammadi, Z., and Hasheminejad-Ahangarani Farahani, Y., Infra-specific genetic and morphological diversity in Linum album (Linaceae), Biologia, 2014, vol. 69, no. 1, pp. 32–39.
Podani, J., Introduction to the Exploration of Multivariate Data, Leiden: Backhuyes Publ., 2000.
Hammer, O., Harper, D.A.T., and Ryan, P.D., PAST: paleontological statistics software package for education and data analysis, Palaeontol. Electronica, 2001, vol. 4, no. 11, art. 4.
Peakall, R. and Smouse, P.E., GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research, Mol. Ecol. Notes, 2006, vol. 6, no. 1, pp. 288–295.
Meirmans, P.G. and Van Tienderen, P.H., GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms, Mol. Ecol. Res., 2004, vol. 4, no. 4, pp. 792–794.
Holsinger, K.E. and Lewis, P.O., HICKORY: a package for analysis of population genetic data V.1.0, Connecticut, USA, 2003. http://darwin.eeb.uconn.edu/ hickory/hickory.html.
Evanno, G., Regnaut, S., and Goudet, J., Detecting the number of clusters of individuals using the software structure: a simulation study, Mol. Ecol., 2005, vol. 14, no. 8, pp. 2611–2620.
Hou, Y. and Lou, A., Population genetic diversity and structure of a naturally isolated plant species, Rhodiola dumulosa (Crassulaceae), PLoS One, 2011, vol. 6, no. 9, p. e24497.
Erfmeier, A., Hantsch, L., and Bruelheide, H., The role of propagule pressure, genetic diversity and microsite availability for Senecio vernalis invasion, PLoS One, 2013, vol. 8, no. 2, p. e57029.
Matesanz, S., Horgan-Kobelski, T., and Sultan, S.E., Phenotypic plasticity and population differentiation in an ongoing species invasion, PLoS One, 2012, vol. 7, no. 9, p. e44955.
Jolivet, C. and Bernasconi, G., Molecular and quantitative genetic differentiation in European populations of Silene latifolia (Caryophyllaceae), Ann. Bot., 2007, vol. 100, no. 1, pp. 119–127.
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Azizi, H., Sheidai, M., Mozaffarian, V. et al. Genetic and morphological diversity in Tragopogon graminifolius DC. (Asteraceae) in Iran. Cytol. Genet. 52, 75–79 (2018). https://doi.org/10.3103/S0095452718010024
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DOI: https://doi.org/10.3103/S0095452718010024