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Peculiarities of modification by astaxanthin of radiation-induced damages in the genome of human blood lymphocytes exposed in vitro on different stages of the mitotic cycle

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Abstract

The features of astaxanthin impact (20 μg/mL) in the culture of human blood lymphocytes exposed to γ-radiation (1.0 Gy) on the G0, S, and G2 phases of the cell cycle were studied using Comet assay. Decrease in the level of DNA damages (Tail Moment index) under astaxanthin influence on lymphocytes irradiated in all stages of cell division was established, while, as a result of previous cytogenetic investigations, lack of the modifying action of astaxanthin after irradiation of cells in the G2 stage and radioprotective effect in the G0 stage of the mitotic cycle had been revealed. In G0 phase, the activation of the processes of apoptosis by astaxanthin in irradiated cells with high levels of genomic damages was found. The obtained data demonstrate that astaxanthin has a powerful radioprotective potential, mainly due to its apoptogenic properties.

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Authors and Affiliations

  1. State Institution “National Research Center for Radiation Medicine,”, National Academy of Medical Sciences of Ukraine, Kyiv, 04050, Ukraine

    D. A. Kurinnyi, O. M. Demchenko & M. A. Pilinska

  2. Educational and Research Center “Institute of Biology and Medicine,”, Taras Shevchenko National University of Kyiv, Kyiv, 01601, Ukraine

    S. R. Rushkovsky

Authors
  1. D. A. Kurinnyi
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  2. S. R. Rushkovsky
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  3. O. M. Demchenko
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  4. M. A. Pilinska
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Correspondence to D. A. Kurinnyi.

Additional information

Original Ukrainian Text © D.A. Kurinnyi, S.R. Rushkovsky, O.M. Demchenko, M.A. Pilinska, 2018, published in Tsitologiya i Genetika, 2018, Vol. 52, No. 1, pp. 52–58.

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Kurinnyi, D.A., Rushkovsky, S.R., Demchenko, O.M. et al. Peculiarities of modification by astaxanthin of radiation-induced damages in the genome of human blood lymphocytes exposed in vitro on different stages of the mitotic cycle. Cytol. Genet. 52, 40–45 (2018). https://doi.org/10.3103/S0095452718010073

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  • DOI: https://doi.org/10.3103/S0095452718010073

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