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Results of Camelus dromedarius and Camelus bactrianus Genotyping by Alpha-S1-Casein, Kappa-Casein Loci, and DNA Fingerprinting

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Abstract

Genotyping of Kazakh camels Camelus dromedarius (milk breed) (n = 18) and Camelus bactrianus (meat breed) (n = 18) by alpha-S1-casein (αs1-CN) and kappa-casein (κ-CN) loci was conducted using the PCR–RFLP analysis method. A new pair of primers was suggested for the amplification of the CSN3 gene fragment with subsequent cleavage of the reaction products by AluI restriction endonuclease in order to identify the gene genetic variants. DNA polymorphism was detected only for the kappa-casein locus; no genetic polymorphism for alpha-S1-casein gene was found in the studied populations. Analysis of the results of DNA fingerprinting demonstrated that the band sharing (BS) coefficient between the groups was low enough (0.13), and the genetic distance (D) between Dromedary and Bactrian breeds was 0.305. The results of genotyping of Bactrian and Dromedary Kazakh camel breeds by alpha-S1-casein, kappa-casein loci, and DNA fingerprinting indicate that the Dromedary breed female camels are more polymorphic as compared with Bactrian.

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Authors and Affiliations

  1. Kazakh National Agrarian University, Almaty, Kazakhstan

    M. E. Yelubayeva, B. A. Buralkhiyev & Y. S. Ussenbekov

  2. Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, St. Petersburg–Pushkin, Russia

    V. I. Tyshchenko & V. P. Terletskiy

  3. Pushkin Leningrad State University, St. Petersburg–Pushkin, Russia

    V. P. Terletskiy

Authors
  1. M. E. Yelubayeva
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  2. B. A. Buralkhiyev
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  3. V. I. Tyshchenko
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  4. V. P. Terletskiy
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  5. Y. S. Ussenbekov
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Corresponding author

Correspondence to Y. S. Ussenbekov.

Additional information

Original Russian Text © M.E. Yelubayeva, B.A. Buralkhiyev, V.I. Tyshchenko, V.P. Terletskiy, Y.S. Ussenbekov, 2018, published in Tsitologiya i Genetika, 2018, Vol. 52, No. 3, pp. 15–24.

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Yelubayeva, M.E., Buralkhiyev, B.A., Tyshchenko, V.I. et al. Results of Camelus dromedarius and Camelus bactrianus Genotyping by Alpha-S1-Casein, Kappa-Casein Loci, and DNA Fingerprinting. Cytol. Genet. 52, 179–185 (2018). https://doi.org/10.3103/S0095452718030040

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  • DOI: https://doi.org/10.3103/S0095452718030040

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