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Verification of Grape Pedigree by Microsatellite Analysis

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Abstract

Microsatellite DNA markers were used to identify and compare the genotypes of 80 samples of grapes and their presumptive parental forms. Both presumptive parental forms were confirmed for 51 samples. Further statistical evaluation showed that the probability of descent from particularly the pair of parents identified at the previous stage (X × Y) was higher than the probability of descent from any random varieties not included in the study. The X × Y index ranged from 103 to 1012. The identity of one of the parents was not confirmed for four samples due to nonmatching allele profiles of the microsatellite loci studied. Genotypes of the ancestral forms were used in pedigree analysis of 22 samples from the set, because plants of the presumptive parental forms were physically unavailable and there was no information on the composition of their microsatellite profiles.

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REFERENCES

  1. This, P., Lacombe, T., and Thomas, M.R., Historical origins and genetic diversity of wine grapes, Trends Genet., 2006, vol. 22, no. 9, pp. 511–519.

    Article  PubMed  CAS  Google Scholar 

  2. Adam-Blondon, A.F., Martinez-Zapater, J.M., and Kole, C., Genetics, Genomics, and Breeding of Grapes, CRC, 2011.

    Google Scholar 

  3. Pelsy, F., Molecular and cellular mechanisms of diversity within grapevine varieties, Heredity, 2010, vol. 104, no. 4, pp. 331–340. doi 10.1038/hdy.2009.161

    Article  PubMed  CAS  Google Scholar 

  4. Lacomb, T., Michel, J., and Laucou, B.V., Large-scale parentage analysis in an extended set of grapevine cultivars (Vitis vinifera L.), Theor. Appl. Genet., 2013, vol. 126, no. 2, pp. 401–414. doi 10.1007/s00122-012-1988-2

    Article  Google Scholar 

  5. Cabezas, J.A., Ibánez, J., Lijavetzky, D., Vélez, D., Bravo, G., Rodríguez, V., Carreco, I., Jermakow, A.M., Carreco, J., Ruiz-García, L., Thomas, M.R., and Martinez-Zapater, J.M., A 48 SNP set for grapevine cultivar identification, BMC Plant Biol.. 2011, vol. 11, p. 153. doi.org/10.1186/1471-2229-11-153

  6. Akkak, A., Boccacci, P., and Botta, R., Cardinal grape parentage: a case of a breeding mistake, Genome, 2007, vol. 50, no. 3, pp. 325–328. doi 10.1139/g06-145

    Article  PubMed  CAS  Google Scholar 

  7. Bowers, J.E. and Meredith, C.P., The parentage of a classic wine grape Cabernet Sauvignon, Nat. Genet., 1997, vol. 16, no. 1, pp. 84–87. doi 10.1038/ng0597-84

    Article  PubMed  CAS  Google Scholar 

  8. Crespan, M., The parentage of Muscat of Hamburg, Vitis, 2003, vol. 42, no. 4, pp. 193–197.

    CAS  Google Scholar 

  9. Boursiquot, J.-M., Lacombe, T., Laucou, V., Julliard, S., Perrin, F.-X., Lanier, N., Legrand, D., Meredith, C., and This, P., Parentage of Merlot and related winegrape cultivars of southwestern France: discovery of the missing link, Aust. J. Grape Wine R, 2009, vol. 15, no. 2, pp. 144–155.

    Article  Google Scholar 

  10. Bautista, J., Dangl, G.S., Yang, J., Reisch, B., and Stover, E., Use of genetic markers to assess pedigrees of grape cultivars and breeding program selections, Am. J. Enol. Vitic., 2008, vol. 59, no. 3, pp. 248–254.

    Google Scholar 

  11. Ashley, M.V., Plant parentage, pollination and dispersal: how DNA microsatellites have altered the landscape, Crit. Rev. Plant Sci., 2010, vol. 29, no. 3, pp. 148–161.

    Article  CAS  Google Scholar 

  12. Di Gaspero, G., Copetti, D., Coleman, C., Castellarin, S.D., Eibach, R., Kozma, P., Lacombe, T., Gambetta, G., Zvyagin, A., Cindrić, P., Kovács, L., Morgante, M., and Testolin, R., Selective sweep at the Rpv3 locus during grapevine breeding for downy mildew resistance, Theor. Appl. Genet., 2012, vol. 124, no. 2, pp. 277–286. doi 10.1007/s00122-011-1703-8

    Article  PubMed  Google Scholar 

  13. De Lorenzis, G., Imazio, S., Biagini, B., Failla, O., and Scienza, A., Pedigree reconstruction of the Italian grapevine Aglianico (Vitis vinifera L.) from Campania, Mol. Biotechnol., 2013, vol. 54, no. 2, pp. 634–642. doi 10.1007/s12033-012-9605-9

    Article  PubMed  CAS  Google Scholar 

  14. Bowers, J.E., Dangl, G.S., and Meredith, C.P., Development and characterization of additional microsatellite DNA markers for grape, Am. J. Enol. Vitic., 1999, vol. 50, no. 3, pp. 243–246.

    CAS  Google Scholar 

  15. Kolesnyk, I.I., Chebotar, S.V., and Volkova, N.A., DNA typing of agricultural crops by microsatellite analysis for the purposes of genotype differentiation, identification and registration, Zb. Nauk. Pratz PBGI, 2015, vol. 25, pp. 213–226.

    Google Scholar 

  16. Galaev, O.V., Shevchuk, G.Yu., Dudchenko, V.V., and Sivilap, Yu.M., Molecular genetic analysis of the soriz genome (Sorghum oryzoidum L.), Cytol. Genet., 2011, vol. 45, no. 4, pp. 208–213.

    Article  Google Scholar 

  17. Lakhneko, O.R., Stepanenko, A.I., and Morgun, B.V., Genotyping Triticum aestivum L. cultivars of Ukraine with microsatellite markers, Fakt. Eksp. Evol. Org., 2016, vol. 19, pp. 61–3.

    Google Scholar 

  18. Ivanovych, Y. and Volkov, R., Genetic relatedness of sweet cherry (Prunus avium L.) cultivars from Ukraine determined by microsatellite markers, J. Horticul. Sci. Biotechnol., 2017, vol. 92, no. 1, pp. 64–72. doi 10.1080/14620316.2017.134256

    Google Scholar 

  19. Bocharova, B.R., Kovaliova, I.A., and Mazurenko, L.S., Identification of grapevine clone genotypes by use of microsatellite markers, Cytol. Genet., 2009, vol. 43, no. 6, pp. 371–379.

    Article  Google Scholar 

  20. Bocharova, V., Mulukina, N., and Kovaliova, I., Differentiation and identification of grapevine accessions of Ukraine by means of molecular markers, Mitt. Klosterneuburg, 2012, vol. 62, no. 4, pp. 154–160.

    CAS  Google Scholar 

  21. Heuertz, M., Goryslavets, S., and Hausman, J.F., Characterization of grapevine accession from Ukraine using microsatellite markers, Am. J. Enol. Vitic., 2008, vol. 59, no. 2, pp. 169–177.

    CAS  Google Scholar 

  22. Risovannaya, V.I., The use of molecular-genetic passports grapes in production, Magarach. Vinograd. Vinodel., 2013, no. 1, pp. 7–9.

  23. Goryslavets, S., Risovanna, V., and Bacilieri, R., A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers, Cytol. Genet., 2010, vol. 44, no. 2, pp. 95–102.

    Article  Google Scholar 

  24. Marshall, T.C., Slate, J., Kruuk, L., and Pemberton, J.M., Statistical confidence for likelihood-based paternity inference in natural populations, Mol. Ecol., 1998, vol. 7, no. 5, pp. 639–655.

    Article  PubMed  CAS  Google Scholar 

  25. Eibach, R., Zyprian, E., Welter, L., and Topper, R., The use of molecular markers for pyramiding resistance genes in grapevine breeding, Vitis, 2007, vol. 46, no. 3, pp. 120–124.

    CAS  Google Scholar 

  26. This, P., Jung, A., Boccacci, P., Borrego, J., Botta, R., Costantini, L., Crespan, M., Dangl, G.S., Eisenheld, C., Ferreira-Monteiro, F., Grando, S., Ibácez, J., Lacombe, T., Laucou, V., Magalhaes, R., Meredith, C.P., Milani, N., Peterlunger, E., Regner, F., Zulini, L., and Maul, E., Development of a standard set of microsatellite reference alleles for identification of grape cultivars, Theor. Appl. Genet., 2004, vol. 109, no. 7, pp. 1448–1458.

    Article  PubMed  CAS  Google Scholar 

  27. Karastan, O.M., Mulyukina, N.A., Plachinda, G.V., and Papina, O.S., The identification and origin of NSC Tairov Research Institute of Viticulture and Winemaking collection seedless grape varieties, Zb. Nauk. Pratz PBGI, 2014, vol. 24, no. 64, pp. 76–84.

    Google Scholar 

  28. Karastan, O.M., Mulyukina, N.A., and Plachinda, G.V., Microsatellite profiles of varieties and forms of grapes in ampelographic collection of NSC Tairov Research Institute of Viticulture and Winemaking, Zb. Nauk. Pratz SGI-NTsN, vol. 29, no. 69, pp. 107–116.

  29. Sefc, K.M., Steinkellner, H., and Wagner, H.W., Application of microsatellite markers to parentage studies in grapevine, Vitis, 1997, vol. 36, no. 4, pp. 179–183.

    Google Scholar 

  30. Crespan, M., Coletta, A., Crupi, P., Giannetto, S., and Antonacci, D., Malvasia nera di Brindisi/Lecce’grapevine cultivar (Vitis vinifera L.) originated from “Negroamaro” and “Malvasia bianca lunga,” Vitis, 2008, vol. 47, no. 4, pp. 205–212.

    CAS  Google Scholar 

  31. Vargas, A.M., Andres, M.T., Borrego, J., and Ibanez, J., Pedigrees of fifty table-grape cultivars, Am. J. Enol. Viticult., 2009, vol. 60, no. 4, pp. 525–532.

    Google Scholar 

  32. Sefc, K.M., Steinkellner, H., Gloss, J., Kampfer, S., and Regner, F., Reconstruction of a grapevine pedigree by microsatellite analysis, Theor. Appl. Genet., 1998, vol. 97, nos. 1–2, pp. 227–231.

    Article  Google Scholar 

  33. Crespan, M., Crespan, G., Giannetto, S., Meneghetti, S., and Costacurta, A., “Vitouska” is the progeny of “Prosecco tondo” and “Malvasia Bianca lunga,” Vitis, 2007, vol. 46, no. 4, pp. 192–194.

    Google Scholar 

  34. Vouillamaoz, J.F., Monaco, A., Constantini, L., Stefanini, M., Scienza, A., and Grando, M.S., The parentage of “Sangiovese”, the most important Italian wine grape, Vitis, 2007, vol. 46, no. 1, pp. 19–22.

    Google Scholar 

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Authors and Affiliations

  1. Tairov Institute of Viticulture and Winemaking National Research Center, 65496, Tairovo, Ovidiopol raion Odessa oblast, Ukraine

    O. M. Karastan, N. A. Muliukina & O. S. Papina

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  1. O. M. Karastan
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  2. N. A. Muliukina
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  3. O. S. Papina
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Correspondence to O. M. Karastan, N. A. Muliukina or O. S. Papina.

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Translated by S. Semenova

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Karastan, O.M., Muliukina, N.A. & Papina, O.S. Verification of Grape Pedigree by Microsatellite Analysis. Cytol. Genet. 52, 331–342 (2018). https://doi.org/10.3103/S0095452718050031

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