Abstract
SNP polymorphism of the TNFα gene’s promoter region at position 824 A→G in Holstein cows of TOO Bayserke-Agro is represented by the following genetic variants: AA, 22.4%; AG, 63.8%; GG, 13.8%; the frequency of alleles A and G was 0.54 and 0.46, respectively. The population under study revealed an excessive frequency of the heterozygous genotype AG, 21.49 individuals, while other genotypes showed a deficit of homozygous variants GG and AA, by 11.16 and 10.32 individuals, respectively. The traits of reproductive function were high in cows with genotype GG: the interval between calving and productive insemination was 259 days, the insemination index was 2.63, the proportion of animals inseminated after more than 91 days was minimal (47.36%) in individuals with the homozygous genotype GG. As an additional criterion for estimating the fertility of bull sperm, the DNA fragmentation method is recommended: the maximum permissible level of sperm cells with DNA fragmentation in frozen sperm is between 13.00 and 17.15%.
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ACKNOWLEDGMENTS
In the part of sample preparation and analysis of the results, this study was carried out with the support of the State Task of the Federal Agency for Scientific Organizations, no. GZ AAAA-A18-118021590138-1. The work on investigating sperm fertility was carried out at the expense of grant funding of the Ministry of Education and Science of the Republic of Kazakhstan on the project topic Intensification of the Breeding Process in Animal Husbandry through the Use of Cellular Reproductive Technology, state registration no. 0115RK00728.
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Conflict of interests. The authors declare that they have no conflict of interest. Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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Bimenova, Z.Z., Ussenbekov, Y.S., Terletskiy, V.P. et al. Reproductive Function of Cows with Different Genotypes for TNFα Locus and Estimation of Sperm Fertility by the DNA Fragmentation Method. Cytol. Genet. 53, 42–48 (2019). https://doi.org/10.3103/S0095452719010043
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DOI: https://doi.org/10.3103/S0095452719010043