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Reporting of B-Chromosomes in Trachyspermum ammi (L.) Sprague (Ajwain)

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Abstract

To the best of our knowledge this is the first study regarding the reporting of B chromosomes in this plant species. During the meiotic study of Trachyspermum ammi (L.) Sprague, maximum two B chromosomes were reported in diploid plants which were retained in induced autotetraploid after the treatment of 0.2% colchicine at seedling stage. In the present study, the meiotic behaviour of B-chromosomes was analyzed in Trachyspermum ammi (L.) Sprague. The presence of B chromosomes and their number in the plant species of higher ploidy level is controversial. The existence of B chromosomes in polyploids varies from species to species. In the present case, no change has been observed in the number of B chromosomes despite the duplication of A chromosomes in autotetraploid plant. The unaffected number of B chromosomes represents their peculiar behaviour of inheritance. The plant species show adaptive mechanism for their retention in population. In the present study, significant changes (p < 0.05) have been observed in the chiasma frequency and pollen fertility of both diploid carrier plant as compare to non-carrier ones. Furthermore, the chromosomal associations have also been considered with observed number of B chromosomes in autotetraploid carrier plant. In autotetraploid carrier plant, the frequencies of multivalents were found to be quite low in comparison to diploid carrier plant.

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REFERENCES

  1. Levin, D.A., Palestis, B.G., Jones, R.N., and Trivers, R., Phyletic hotspots for B chromosomes in angiosperms, Evolution, 2005, vol. 59, pp. 962–969.

    Article  PubMed  Google Scholar 

  2. Houben, A. and Carchilan, M., Plant B chromosomes: what makes them different?, in Plant Cytogenetics, Bass, H.W. and Birchler, J.A., Eds., Plant Genetics 59 and Genomics: Crops and Models 4, 2012.

  3. Perfectti, F. and Werren, J.H., The interspecific origin of B chromosomes: experimental evidence, Evolution, 2001, vol. 55, no. 5, pp. 1069–1073.

    Article  CAS  Google Scholar 

  4. Camacho, J.P.M., Sharbel, T.F., and Beukeboom, L.W., B-chromosome evolution, Philos. Trans. R. Soc. B Biol. Sci., 2000, vol. 355, pp. 163–178.

    CAS  Google Scholar 

  5. Maluszynska, J. and Schweizer, D., Ribosomal RNA genes in B chromosomes of Crepis capillaries detected by non-radioactive in situ hybridization, Heredity, 1989, vol. 62, pp. 59–65.

    Article  PubMed  Google Scholar 

  6. Stitou, S., Diaz de La Guardia, R., Jimenez, R., and Burgos, M., Inactive ribosomal cistrons are spread throughout the B chromosomes of Rattus rattus (Rodentia, Muridae). Implications for their origin and evolution, Chromosome Res., 2000, vol. 8, pp. 305–311.

    Article  CAS  PubMed  Google Scholar 

  7. van Vugt, J., de Nooijer, S., Stouthamer, R., and de Jong, H., NOR activity and repeat sequences of the paternal sex ratio chromosome of the parasitoid wasp Trichogramma kaykai, Chromosoma, 2005, vol. 114, pp. 410–419.

    Article  CAS  PubMed  Google Scholar 

  8. Green, D.M., Muller’s rachet and the evolution of supernumerary chromosomes, Genome, 1990, vol. 33, pp. 818–824.

    Article  Google Scholar 

  9. Jones, R.N., Gonzalez-Sanchez, M., Gonzalez-Garcia, M., Vega, J.M., and Puertas, M.J., Chromosomes with a life of their own, Cytogenet. Genome Res., 2008, vol. 120, pp. 265–280.

    Article  CAS  PubMed  Google Scholar 

  10. Beukeboom, L.W., Bewildering Bs: an impression of the 1st B-Chromosome Conference, Heredity, 1994, vol. 73, pp. 328–336.

    Article  Google Scholar 

  11. Jones, R.N., Tansley Review No. 85: B chromosomes in plants, New Phytol., 1995, vol. 131, pp. 411–434.

    Article  Google Scholar 

  12. Jones, R.N., B-chromosome drive, Am. Nat., 1991, vol. 137, pp. 430–442.

    Article  Google Scholar 

  13. Dhar, M.K., Friebe, B., Koul, A.K., and Gill, B.S., Origin of an apparent B chromosome by mutation, chromosome fragmentation and specific DNA sequence amplification, Chromosoma, 2002, vol. 111, pp. 332–340.

    Article  CAS  PubMed  Google Scholar 

  14. Staub, R.W., Leaf striping correlated with the presence of B chromosomes in maize, J. Hered., 1987, vol. 78, pp. 71–74.

    Article  Google Scholar 

  15. Gill, J.J.B., Jones, B.M.G., Marchant, C.J., NeLeish, J., and Ockendoy, D.J., The distribution of chromosome races of Ranunculus ficaria L. in the British Isles, Ann. Bot., 1972, vol. 36, pp. 31–47.

    Article  Google Scholar 

  16. Sharma, A.K. and Aiyangar, H.R., Occurrence of B chromosomes in diploid Allium stracheyii Baker and their elimination in polyploids, Chromosoma (Berl.), 1961, vol. 12, pp. 310–317.

    Article  Google Scholar 

  17. Papes, D., B chromosomes of the genus Leucanthenum in Yugoslavia, Acata Biol. Yugoslav., Genet, 1971, vol. 3, pp. 261–270.

    Google Scholar 

  18. Bocher, T.W., Chromosome behaviour and syncyte formation in Phleum phleoides (L.), Bot. Notiser, 1950, pp. 353–368.

    Google Scholar 

  19. Hakanson, A., Behaviour of accessory rye chromosome in the embryo-sac, Hereditas, 1948, vol. 34, nos. 1/2, pp. 35–59.

    Article  Google Scholar 

  20. Hakanson, A., Embryology of Poa alpina plants with accessory chromosomes, Hereditas, 1948, vol. 34, no. 1, pp. 233–247.

    Article  Google Scholar 

  21. Hakanson, A., Transmission of accessory chromosomes in Poa alpina, Hereditas, 1954, vol. 40, pp. 523–526.

    Google Scholar 

  22. Muntzing, A., Different chromosome numbers in root tips and pollen mother cells in a sexual strain of Poa alpina, Hereditas, 1946, vol. 32, pp. 127–129.

    Google Scholar 

  23. Muntzing, A., Accessory chromosomes in Poa alpina, Heredity, 1948, vol. 2, no. 1, pp. 60–61.

    Article  Google Scholar 

  24. Muntzing, A., Some recent data on accessory chromosomes in Secale and Poa. Chromosomes Today, 1966, vol. 1, pp. 7–14.

    Google Scholar 

  25. Haga, T., Intra-individual variation in number and linear patterning of the chromosomes I. B chromosomes in Rumex paris and Scilla Proc. Japan. Acad., 1961, vol. 37, no. 10, pp. 627–632.

    Article  Google Scholar 

  26. Teppner, H., Cytosystematik, bimodale Chromosomensatze and Permanentanorthoploidie bei Onosma (Boraginaceae), Ost. But. Z., 1971, vol. 119, pp. 196–233.

    Article  Google Scholar 

  27. Raghuvanshi, S.S. and Pant, M., (nee' Upreti), Response of B chromosomes to induced tetraploid in Trigonella-foenum-graecum, Perspect. Cytol. Genet., 1981. V. 3. P. 167–170.

    Google Scholar 

  28. Raghuvanshi, S.S. and Mahajan, S., Chromosomal association in B carrier and non-carrier diploid, tetraploid and octaploid Impatiens balsamina L., Ind. Nat. Sci. Acad., 1982, vol. 48, pp. 147–151.

    Google Scholar 

  29. Kootin-Sanwu, M., Populations and hybrids in Caltha palustris, Chromosomes Today, 1966, vol. 1, p. 266.

    Google Scholar 

  30. Kootin-Sanwu, M. and Woodell, S.R.J., The cytology of Caltha palustris L. (Sensulato): distribution and behaviour of the chromosome races, Caryologia, 1970, vol. 23, pp. 225–239.

    Google Scholar 

  31. Kootin-Sanwu, M., The cytology of Caltha palustris. Cytogenetic relationships, Heredity, 1971, vol. 26, pp. 121–135.

    Article  Google Scholar 

  32. Jones, R.N. and Rees, H., Genotypic control of chromosome behavior in Rye XI. The influence of B chromosomes on meiosis, Heridity, 1967, vol. 22, pp. 333–346.

    Article  Google Scholar 

  33. Jones, R.N. and Rees, H., B Chromosomes, London: Academic. 1982, 1st ed.

    Google Scholar 

  34. Jayalakshmi, K. and Pantulu, J.V., The effect of B-chromosome on A-chromosome chiasma formation in pearl millet, Cytologia, 1984, vol. 49, pp. 635–643.

    Article  Google Scholar 

  35. Rao, M.V.S. and Pantulu, J.V., The effect of B chromosome on meiosis in pearl millet Pennisetum typhoides, Chromosoma (Berl.), 1978, vol. 69, pp. 121–130.

    Article  Google Scholar 

  36. Raghuvanshi, S.S. and Kumar, G., No male sterility gene on B-chromosome in Plantago coronopus, Heredity, 1983, vol. 51, pp. 429–433.

    Article  Google Scholar 

  37. Hanson, G.P., B-chromosome stimulated crossing over in maize, Genetics, 1969, vol. 63, pp. 601–609.

    CAS  PubMed  PubMed Central  Google Scholar 

  38. Ward, E.J., The heterochromatic B-chromosome of maize: the segments affecting recombination, Chromosoma, 1973, vol. 43, pp. 177–186.

    Article  Google Scholar 

  39. Kumar, G. and Singh, V., Influence of B-chromosomes on genetic recombination and fertility in pearl millet, Cytologia, 2004, 69. P. 431–437.

    Article  Google Scholar 

  40. Jackson, R.C. and Newmark, P., Effects of supernumerary chromosomes on production of pigment in happlopappus gracilis. Science, New York, 1960, vol. 132.

  41. Pant, M. and Raghuvanshi, S.S., Chromosomal association in C1, C2 and C8 auto-tetraploid B carrier Trigonella foenum-graceum L., J. Cytol. Genet., 1980, vol. 15, pp. 93–98.

    Google Scholar 

  42. Paliwal, R.L. and Hyde, B.B., The association of a single B chromosome with male sterility in Plantago coronopus, Am. J. Bot., 1959, vol. 46, pp. 460–466.

    Article  Google Scholar 

  43. Kumar, G. and Dikshit, A., Influence of B chromosomes on chiasma frequency in Impatiens balsamina, J. Cytol. Genet., 2002, vol. 3, pp. 101–104.

    CAS  Google Scholar 

  44. Bala, S., Kaushal, B., and Gupta, R.C., Effect of B‑chromosomes on meiotic behaviour and genetic recombination in Artemisia nilagirica (C. B. Clarke) Pamp., Chromosome Botany, 2012, vol. 7, pp. 9–12.

    Article  Google Scholar 

  45. Carlson, W.R., Roseman, R., and Zheng, Y., Localizing a region on the B chromosome that influences crossing over, Maydica, 1993, vol. 38, pp. 107–113.

    Google Scholar 

  46. Evans, G.M. and Macefield, A.J., Suppression of homoeologous pairing by B chromosomes in a Lolium species hybrid, Nat. New Biol., 1972, vol. 236, pp. 110–111.

    Article  CAS  PubMed  Google Scholar 

  47. Evans, G.M. and Macefield, A.J., The effect of B chromosomes on homoeologous pairing in species hybrids. I. Lolium temulentum x Lolium perenne, Chromosoma, 1973, vol. 41, pp. 63–73.

    Article  Google Scholar 

  48. Marschner, S., Kumke, K., and Houben, A., B chromosomes of B. dichromosomatica show a reduced level of euchromatic histone H3 methylation marks, Chromosome Res., 2007, vol. 15, pp. 215–222.

    Article  CAS  PubMed  Google Scholar 

  49. Morais-Cecilio, L., Delgado, M., Jones, R.N., and Viegas, W., Painting rye B chromosomes in wheat: interphase chromatin organization, nuclear disposition and association in plants with two, three or four Bs, Chromosome Res., 1996, vol. 4, pp. 195–200.

    Article  CAS  PubMed  Google Scholar 

  50. Bougourd, S.M. and Jones, R.N., B chromosomes: a physiological enigma, New Phytol., 1997, vol. 137, pp. 43–54.

    Article  Google Scholar 

  51. Muntzing, A., Accessory chromosomes in Secale and Poa, in Proc. VIII Int. Congr. Genet., Hereditas, 1949, suppl. vol., pp. 402–411.

  52. Jones, R.N., Viegas, W., and Houben, A., A century of B chromosomes in plants: so what?, Ann. Bot. (Lond.), 2008, vol. 111, pp. 767–775.

    Article  Google Scholar 

  53. Birchler, J.A., Yu, W., and Han, F., Plant engineered minichromosomes and artificial chromosome platforms, Cytogenet. Genome Res., 2008, vol. 120, pp. 228–232.

    Article  CAS  PubMed  Google Scholar 

  54. Yu, W., Han, F., Gao, Z., Vega, J.M., and Birchler, J.A., Construction and behavior of engineered minichromosomes in maize, Proc. Natl. Acad. Sci. U. S. A., 2007, vol. 104, pp. 8924–8929.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  55. Yu, W., Lamb, J.C., Han, F., and Birchler, J.A., Telomere-mediated chromosomal truncation in maize, Proc. Natl. Acad. Sci. U. S. A., 2006, vol. 103, pp. 17331–17336.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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ACKNOWLEDGMENTS

Authors are thankful to NRCSS, Ajmer, Rajasthan, India for supplying seeds of T. ammi var. AA-1. One of the authors (Harshita Dwivedi) is extremely thankful to University Grant Commission (UGC) for financial assistance. Sincere thanks to all the members of Plant Genetics Laboratory for their encouragement and support.

 COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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  1. Plant Genetics Laboratory, Department of Botany, University of Allahabad, 211002, Allahabad, India

    Harshita Dwivedi &  Girjesh Kumar

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  1. Harshita Dwivedi
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Correspondence to Harshita Dwivedi.

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Harshita Dwivedi, Girjesh Kumar Reporting of B-Chromosomes in Trachyspermum ammi (L.) Sprague (Ajwain). Cytol. Genet. 53, 68–75 (2019). https://doi.org/10.3103/S0095452719010079

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