Abstract
Streptomyces albus J1074 is one of the most popular chassis for heterologous expression of actinobacterial biosynthetic gene clusters (BGCs). Considerable efforts are invested into understanding all the physiological and genetic aspects of secondary metabolism of J1074 in order to maximize the expression of heterologous BGCs. It has to be noted that the J1074 genome itself is home to numerous (>20) BGCs, whose expression varies widely. Therefore, the identification of the factors limiting the expression of J1074 BGCs might help improve this strain for heterologous expression purposes. As first steps towards this goal, herein we describe the secondary metabolome of J1074 in liquid medium SG2, previously shown by us to support the production of antibacterial and antifungal compounds. We compare the results of metabolomic studies with the transcriptome of J1074 in SG2 after 60 h of growth. Results of our studies are discussed in the context of current knowledge on the J1074 transcriptome and metabolome data.
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ACKNOWLEDGMENTS
This work was supported by grants Bg-41Nr, M/26-2018 (to B.O. Ostash) and BG-46F (to V.O. Fedorenko), all from Ministry of Education and Science of Ukraine. Ostash B.O. was supported by DAAD fellowship. Süssmuth R.D. and Ostash B.O. thank the Bundesministerium für Bildung und Forschung (BMBF) project: 021DK17013 AWAKEDRUGS for funding.
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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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Koshla, O.T., Rokytskyy, I.V., Ostash, I.S. et al. Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2. Cytol. Genet. 53, 1–7 (2019). https://doi.org/10.3103/S0095452719010080
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DOI: https://doi.org/10.3103/S0095452719010080