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The Effect of Platelet-Rich Plasma on Morphogenesis and Expression of Chondrogenic Marker Genes by Chondrocyte-Like Cells of the Rat Nucleus Pulposus in Vitro

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Abstract

The aim of the study was to investigate the effect of 3, 10, and 20% of platelet-rich rat plasma (PRP) on the morphological characteristics of chondrocyte-like cells of the rat nucleus pulposus (NP) and the expression of chondrogenic genes ACAN, COLII, GPC3, MGP, PTN, ANXA3, and VIM in vitro. A dose-dependent increase of NP cell viability was observed in cultures supplemented with 3, 10, and 20% PRP. Platelets showed tropicity to blast-like cells in culture and formed adhesion zones. Differentiation of blast-like cells was observed and dividing cells were identified on day 14 of cultivation with 20% PRP. PRP prevented the decrease of chondrogenic gene expression in a dose-dependent manner, with 20% PRP being the most efficient. Expression of the majority of chondrogenic genes studied was observed in these cultures: the expression of COLII increased 2.9 times (P = 0.002); that of VIM, 2.1 times (P = 0.0003); that of ANXA3, 2.3 times (P = 0.0003); that of GPC3, 1.4 times (P = 0.0011); that of PTN, 1.6 times (P = 0.0017); and that of ACAN, 1.2 times (P = 0.0606).

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Conflict of interests. The authors declare that they have no conflict of interest. Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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  1. Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine, 04050, Kyiv, Ukraine

    E. G. Pedachenko, I. G. Vasilyeva, M. V. Khizniak, N. G. Chopyck, N. P. Oleksenko, I. N. Shuba, O. I. Tsjubko, O. S. Galanta, N. D. Snitsar, T. A. Makarova & A. B. Dmytrenko

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  1. E. G. Pedachenko
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  2. I. G. Vasilyeva
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  3. M. V. Khizniak
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  4. N. G. Chopyck
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Correspondence to E. G. Pedachenko, I. G. Vasilyeva, M. V. Khizniak, N. G. Chopyck, N. P. Oleksenko, I. N. Shuba, O. I. Tsjubko, O. S. Galanta, N. D. Snitsar, T. A. Makarova or A. B. Dmytrenko.

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Pedachenko, E.G., Vasilyeva, I.G., Khizniak, M.V. et al. The Effect of Platelet-Rich Plasma on Morphogenesis and Expression of Chondrogenic Marker Genes by Chondrocyte-Like Cells of the Rat Nucleus Pulposus in Vitro. Cytol. Genet. 53, 124–131 (2019). https://doi.org/10.3103/S0095452719020087

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