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Calcium-Dependent Changes in Cellular Redox Homeostasis and Heat Resistance of Wheat Plantlets under Influence of Hemin (Carbon Monoxide Donor)

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Abstract

Carbon monoxide (CO) is considered to be an important molecule-gasotransmitter involved in the regulation of the functional activity of plants, including in the processes of adaptation to stress factors. However, the associations of CO with other participants of signaling in the plant cells remain poorly studied. Using an inhibitory method, the role of different calcium pools in realization of the influence of hemin (carbon monoxide donor) on generation and neutralization of reactive oxygen species (ROS) in the root cells of wheat (Triticum aestivum L.) plantlets and their resistance to damaging heating (45°C, 10 min) was studied. The treatment of plantlets with 5 μM hemin caused a transient increase in the activity of extracellular peroxidase in roots and an increase in the generation of ROS with the maximum in 1.5–2 h after the beginning of treatment. The chelator of extracellular calcium EGTA and the inhibitor of inositol-1,4,5-phosphate formation neomycin, which reduces calcium influx into the cytosol from intracellular compartments, almost completely eliminated an increase in the activity of extracellular peroxidase caused by exogenous CO. At the same time, EGTA (completely) and neomycin (partially) leveled an increase in the content of hydrogen peroxide in the roots of plantlets occurring under the influence of CO donor. The treatment of plantlets with hemin also induced an increase in the activity of superoxide dismutase, catalase, and intracellular peroxidase in roots. Both calcium antagonists eliminated these effects. In the presence of EGTA and neomycin, there was also no positive effect of the treatment of hemin on the state of biomembranes and survival of plantlets after the damaging heating. It was concluded that both extracellular calcium and that deposited in intracellular compartments is involved in CO donor-induced increase in the formation of ROS, induction of the antioxidant system, and development of heat resistance of wheat plantlets.

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Authors and Affiliations

  1. Dokuchaev Kharkiv National Agrarian University, 62483, Kharkiv, Ukraine

    M. A. Shkliarevskyi, Yu. V. Karpets, Yu. E. Kolupaev & A. A. Lugovaya

  2. Karazin Kharkiv National University, 61022, Kharkiv, Ukraine

    Yu. E. Kolupaev

  3. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, 03143, Kyiv, Ukraine

    A. P. Dmitriev

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  1. M. A. Shkliarevskyi
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  2. Yu. V. Karpets
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  3. Yu. E. Kolupaev
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  4. A. A. Lugovaya
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Shkliarevskyi, M.A., Karpets, Y.V., Kolupaev, Y.E. et al. Calcium-Dependent Changes in Cellular Redox Homeostasis and Heat Resistance of Wheat Plantlets under Influence of Hemin (Carbon Monoxide Donor). Cytol. Genet. 54, 522–530 (2020). https://doi.org/10.3103/S0095452720060109

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