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Phylogenetic Analysis of the Bacillus subtilis IFBG MK-2 Strain and Riboflavin Production by Its Induced Clones

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Abstract

The aim of this work was to study phylogenetic relationships between the new producer strain Bacillus subtilis IFBG MK-2 and the existing strains and increase the biosynthetic activity of this producer by mutagenesis. As a result of sequencing the 16S rRNA gene of the IFBG MK-2 strain, gene fragments with a total length of 1305 nucleotides have been obtained. Based on the 16S rRNA gene nucleotide sequences, a phylogenetic analysis was performed and a dendrogram was constructed using the Neighbor Joining method and the Kimura two-parameter model. It has been shown that the similarity between the sum of the 16S rRNA gene sequenced fragments of the IFBG MK-2 strain and the GenBank-deposited sequence of this gene of the B. subtilis IAM 12118 type strain reaches 99%. Based on the phylogenetic analysis, the IFBG MK-2 strain was identified as B. subtilis. Through induced mutagenesis, using UV radiation and subsequent stepwise selection, a mutant clone B. subtilis IFBG MK-1A providing for a 50% increase in riboflavin production compared with the original strain has been obtained.

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Funding

The study was supported by the Creation of Overproducer Strains for Secondary Metabolites (Amino Acids, Alcohols, and Vitamins) departmental program. State registration no. 0119U101489.

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Authors and Affiliations

  1. Institute for Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, 04123, Kyiv, Ukraine

    M. M. Radchenko, O. O. Tigunova, N. Ye. Beiko, H. S. Andriiash & S. M. Shulga

  2. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 03143, Kyiv, Ukraine

    L. B. Zelena

Authors
  1. M. M. Radchenko
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  2. O. O. Tigunova
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  3. L. B. Zelena
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  4. N. Ye. Beiko
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  5. H. S. Andriiash
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  6. S. M. Shulga
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Corresponding author

Correspondence to S. M. Shulga.

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The authors declare that they have no conflict of interests.

This article contains no results of any investigations involving humans and animals as their objects.

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Translated by N. Tarasyuk

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Radchenko, M.M., Tigunova, O.O., Zelena, L.B. et al. Phylogenetic Analysis of the Bacillus subtilis IFBG MK-2 Strain and Riboflavin Production by Its Induced Clones. Cytol. Genet. 55, 145–151 (2021). https://doi.org/10.3103/S0095452721020134

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