Abstract
Allelic variants were identified using SSR-markers, and locus-specific features of the genetic structure of the round goby (Neogobius melanostomus) populations from five samples of the Black and Azov Sea basin were established. When comparing the values of observed Ho and expected He heterozygosity, it was found that the lowest average value for all analyzed loci was noted in the sample of Lake Yalpug (0.69 and 0.60, respectively), and the highest was in the sample of Zmeyniy Island (0.89 and 0.83). The average polymorphism information content (PIC) ranged from 0.64 for Ame129 locus to 0.68 for Ame133, which indicates a high level of polymorphism of selected markers for this fish species. The phylogenetic analysis showed that the main cluster is formed by representatives of the round goby of the localities Zmeyniy Island and Dzharilgatsky Bay (identity was 0.33) as well as the Dnieper-Bug estuary (0.26). The similarity of the round goby of Lake Yalpug with representatives of the main cluster is 0.23. Pisces of this locality occupy a separate position on the dendrogram, which indicates the specificity of their genetic structure that was formed historically under the influence of abiotic factors of the studied geographical zones during prolonged adaptation.
Similar content being viewed by others
REFERENCES
Adrian-Kalchhauser, I., Blomberg, A., Larsson, T., et al., The round goby genome provides insights into mechanisms that may facilitate biological invasions, BMC Biol., 2020, vol. 18, no. 1, pp. 1–33. https://doi.org/10.1186/s12915-019-0731-8
Brandner, J., Cerwenka, A., Schliewen, U.K., et al., Invasion strategies in round goby (Neogobius melanostomus): is bigger really better?, PLoS One, 2018, vol. 13. e0190777. https://doi.org/10.1371/journal.pone.0190777
Brown, J.E. and Stepien, C.A., Ancient divisions, recent expansions: phylogeography and population genetics of the round goby Apollonia melanostoma, Mol. Ecol., 2008, vol. 17, pp. 2598–2615. doi . 2008.03777.xhttps://doi.org/10.1111/j.1365-294X
Burkett, E.M. and Jude, D.J., Long-term impacts of invasive round goby Neogobius melanostomus on fish community diversity and diets in the St. Clair River, Michigan, J. Great Lakes Res., 2015, vol. 41, no. 3, pp. 862–872. https://doi.org/10.1016/j.jglr.2015.05.004
Cerwenka, A.F., Brandner, J., Geist, J., et al., Strong versus weak population genetic differentiation after a recent invasion of gobiid fishes (Neogobius melanostomus and Ponticola kessleri) in the upper Danube, Aquat. Invasions, 2014, vol. 9, pp. 71–86. https://doi.org/10.3391/ai.2014.9.1.06
Cristescu, M.E., Genetic reconstructions of invasion history, Mol. Ecol., 2015, vol. 24, pp. 2212–2225. https://doi.org/10.1111/mec.13117
Demchenko, V.O. and Tkachenko, M.Y., Biological characteristics of the round goby, Neogobius melanostomus (Pallas, 1814), from different water bodies, Arch. Pol. Fish., 2017, vol. 25, no. 1, pp. 51–61. https://doi.org/10.1515/aopf-2017-0006
Farwell, M., Hughes, G., Smith, J.L., et al., Differential female preference for individual components of a reproductive male round goby (Neogobius melanostomus) pheromone, J. Great Lakes Res., 2017, vol. 43, no. 2, pp. 379–386. https://scholar.uwindsor.ca/biologypub/234.
Feldheim, K.A., Willink, P., Brown, J.E., et al., Microsatellite loci for Ponto-Caspian gobies: markers for assessing exotic invasions, Mol. Ecol. Resour., 2009, vol. 9, no. 2, pp. 639–644. https://doi.org/10.1111/j.1755-0998.2008.02495.x
Gorev, L.M., Peleshenko, V.I., and Khilchevsky, V.K., Hydrochemistry of Ukraine, Kyiv: High School, 1995.
Hempel, M., and Thiel, R., First records of the round goby Neogobius melanostomus (Pallas, 1814) in the Elbe River, Germany, BioInvasions Records, 2013, vol. 2, pp. 291–295. https://doi.org/10.3391/bir.2013.2.4.05
Janáč, M, Valová, Z., Roche, K., et al., No effect of round goby Neogobius melanostomus colonisation on young-of-the-year fish density or microhabitat use, Biol. Invasions, 2016, vol. 18, pp. 2333–2347. https://doi.org/10.1007/s10530-016-1165-7
Janáč, M., Roche, K., Šlapansky, L., et al., Long-term monitoring of native bullhead and invasive gobiids in the Danubian rip-rap zone, Hydrobiologia, 2017a, vol. 807, no. 1, pp. 263–275. https://doi.org/10.1007/s10750-017-3398-6
Janáč, M., Bryja, J., Ondračková, M., et al., Genetic structure of three invasive gobiid species along the Danube–Rhine invasion corridor: similar distributions, different histories, Aquat. Invasions, 2017b, vol. 12, pp. 551–564. https://doi.org/10.3391/ai.2017.12.4.11
Johansson, M.L., Dufour, B.A., Wellband, K.W., et al., Human-mediated and natural dispersal of an invasive fish in the eastern Great Lakes, Heredity, 2018, vol. 120, no. 6, pp. 533–546. https://doi.org/10.1038/s41437-017-0038-x
Kornis, M.S., Mercado-Silva, N., and Vander Zanden, M.J., Twenty years of invasion: a review of round goby Neogobius melanostomus biology, spread and ecological implications, J. Fish Biol., 2012, vol. 80, pp. 235–285. https://doi.org/10.1111/j.1095-8649.2011.03157.x
Kumar, S., Stecher, G., Li, M., et al., MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms, Mol. Biol. Evol., 2018, vol. 35, no. 6, pp. 1547–1549. https://doi.org/10.1093/molbev/msy096
Kuznetsov, V.M., Wright’s F-statistics: evaluation and interpretation, Probl. Biol. Product. Anim., 2014, vol. 4, pp. 80–104.
Lukashov, V.V., Molecular Evolution and Phylogenetic Analysis, Moscow: BINOM, 2009.
Maqsood, H.M. and Ahmad, S.M., Advances in molecular markers and their applications in aquaculture and fisheries, GenAqua, 2017, vol. 1, pp. 27–41. https://doi.org/10.4194/2459-1831-v1_1_05
Morissette, O., Paradis, Y., Pouliot, R., et al., Spatio-temporal changes in littoral fish community structure along the St. Lawrence River (Quebec, Canada) following round goby (Neogobius melanostomus) invasion, Aquat. Invasion, 2018, vol. 13, pp. 501–512. https://doi.org/10.3391/ai.2018.13.4.08
Nagy, S., Poczai, P., Cernák, I., et al., PICcalc: an online program to calculate polymorphic information content for molecular genetic studies, Biochem. Genet., 2012, vol. 50, nos. 9–10, pp. 670–672. https://doi.org/10.1007/s10528-012-9509-1
Nei, M., Estimation of average heterozygosity and genetic distance from a small number of individuals, Genetics, 1978, vol. 89, no. 3, pp. 583–590.
Nei, M., Phylogenetic analysis in molecular evolutionary genetics, Annu. Rev. Genet., 1996, vol. 30, pp. 371–403. https://doi.org/10.1146/annurev.genet.30.1.371
Peakall, R. and Smouse, P.E., GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update, Bioinformatics, 2012, vol. 28, pp. 2537–2539. https://doi.org/10.1093/bioinformatics/bts460
Raab, D., Mandrak, N.E., and Ricciardi, A., Low-head dams facilitate Round Goby Neogobius melanostomus invasion, Biol. Invasions, 2018, vol. 20, pp. 757–776. https://doi.org/10.1007/s10530-017-1573-3
Snyder, M.R. and Stepien, C.A., Genetic patterns across an invasion’s history: a test of change versus stasis for the Eurasian round goby in North America, Mol. Ecol., 2017, vol. 26, no. 4, pp. 1075–1090. https://doi.org/10.1111/mec.13997
Szalyky, Z., Bammer, V., Gyorgy, A., et al., Offshore distribution of invasive gobies (Pisces: Gobiidae) along the longitudinal profile of the Danube River, Fundam. Appl. Limnol., 2015, vol. 187, no. 2, pp. 127–133. https://doi.org/10.1127/fal/2015/0768
Tserkova, F., Kirilova, I., Tcholakova, T., et al., Comparative study of round goby (Neogobius melanostomus) populations inhabiting Black Sea and North-West European water basins as revealed by variability in cytochrome b gene, Bulgar. J. Agric. Sci., 2015, vol. 21, no. 1, pp. 100–105.
Tserkova, F., Gevezova-Kazakova, M., Gospodinov, G., et al., Genetic diversity and geographic distribution of round goby Neogobius melanostomus (Pallas, 1814) (Perciformes: Gobiidae) as revealed by mtDNA cyt b gene haplotypes, Acta Zool. Bulg., Suppl., 2017, pp. 41–45.
White, N.J., Snook, R.R., and Eyres, I., The past and future of experimental speciation, Trends Ecol. Evol., 2020, vol. 35, pp. 10–21. https://doi.org/10.1016/j.tree.2019.08.009
Zamorov, V.V. and Radionov, D.B., Polymorphism at the β-esterase locus of the Neogobius melanostomus goby of the Odessa Bay and the waters of the Snake Island, Hydrobiol. J., 2014, vol. 50, no. 3, pp. 67–77. http://nbuv.gov.ua/UJRN/gbj_2014_50_3_8
Zamorov, V.V., Radionov, D.B., Kucherov, V.A., et al., The dynamics of genetic structure of round goby Neogobius melanostomus (Pallas) groupings in the Odessa Bay of the Black Sea utilizing biochemical marker loci, Acta Biol. Univ. Daugav., 2017, vol. 17, no. 2, pp. 257–264.
Zamorov, V.V., Karavanskiy, Y., Leonchyk, Y., et al., The effect of atmospheric pressure and water temperature on the swimming activity of round goby, Neogobius melanostomus (Actinopterygii: Perciformes: Gobiidae), Acta Ichthyol. Piscat., 2018, vol. 48, no. 4, pp. 373–379. https://doi.org/10.3750/AIEP/02445
Funding
This study was performed at the expense of Ministry of Education and Science obtained for research (basic research) on “Patterns of Differentiation of Gobiidae Populations at the Morphological and Genetic Levels in the Black Sea–Azov Basin of Ukraine” in 2017–2019.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interests. The authors declare that they have no conflict of interests.
Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The study was performed in accordance with the European Convention for the protection of vertebrate animals used for experimental and other scientific purposes (Strasbourg, 1986) ETS no. 123.
Additional information
Translated by V. Mittova
About this article
Cite this article
Tarasjuk, S.I., Zamorov, V.V., Zaloilo, O.V. et al. Genetic Differentiation of the Round Goby (Neogobius melanostomus) from Certain Localities of the Black and Azov Sea Basin Using Microsatellite Loci. Cytol. Genet. 55, 236–247 (2021). https://doi.org/10.3103/S0095452721030105
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0095452721030105