Skip to main content
SpringerLink
Log in

Molecular and Spectroscopic Evaluation of the Effects of Coumarin on Lentil

  • Published:
Cytology and Genetics Aims and scope Submit manuscript

Abstract

This study examines the genotoxic effects of coumarin (2H-1-benzopyran-2-one) on Lens culinaris Medik cv Sultan in terms of DNA polymorphism and protein quantification. Effective concentration values were calculated according to a probit model, which is a type of regression where the dependent variable can take only two values, i.e., life or death, after 48 or 72 h of treatment. Based on this analysis, the effective concentration value of EC50 identified as approximately 278 μM, and then adjusted to 300 μM. The bulb roots were treated with 300 μM (EC50), 600 μM (2X EC50) concentrations and the Hoagland was used in the control group.10 RAPD primers were used and as a result of the analysis, it was observed that 2 monomorphic and 8 polymorphic band profile primers for treatment of 600 and 300 μM, coumarin concentration according to the control group. Total of 39 polymorphic bands were detected from total of 97 bands and the percentage of polymorphism was detected as 48.75%. SDS-PAGE analysis for total protein profile showed that there were differences between the treatment groups. In addition to PCR analysis, the root samples were examined by Fourier transform infrared (FT-IR) spectroscopy in order to determine the effects of coumarin on the quantity of biomolecules structure of L. culinaris One-way analysis of variance (ANOVA) was used in the calculation of statistical differences between the groups (P < 0.05).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.

Similar content being viewed by others

REFERENCES

  1. Ana, A.S., Penelope, G.A., Hugo, M., et al., The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors, Plant Signal. Bah., 2011, vol. 6, pp. 1104–1110. https://doi.org/10.4161/psb.6.8.15793

    Article  CAS  Google Scholar 

  2. Atienza, F.A., Conradie, M., Evenden, A.J., et al., Qualitative assessment of genotoxicity using random amplified polymorphic DNA: comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene, Env. Tox. Chem., 1999, vol. 18, pp. 2275–2282. https://doi.org/10.1002/etc.5620181023

    Article  Google Scholar 

  3. Atienzar, F.A., Venier, P., Jha, A.N., et al., Evaluation of the random amplified polymorphic DNA (RAPD) assay for the detection of DNA damage and mutations, Mut. Res./Gen Tox. Env. Mut., 2002, vol. 521, pp. 151–163. https://doi.org/10.1016/S1383-5718(02)00216-4

    Article  CAS  Google Scholar 

  4. Atienzar, F.A. and Jha, A.N., The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: a critical review, Mut. Res. Rev., 2006, vol. 613, pp. 76–102. https://doi.org/10.1016/j.mrrev.2006.06.001

    Article  CAS  Google Scholar 

  5. Barun, S.G., Bjorn, P.J., Tao, G., et al., Application of ATR-FTIR spectroscopy to compare the cell materials of wood decay fungi with wood mould fungi, Int. J. Spectrosc., 1999, vol. 2015, pp. 1–8. https://doi.org/10.1155/2015/521938

    Article  CAS  Google Scholar 

  6. Borgaud, F., Hehn, A., Larbat, R., et al., Biosynthesis of coumarins in plants: a major pathway still to be unraveled for cytochrome P450 enzymes, Phytochem. Rev., 2006, vol. 5, pp. 293–308. https://doi.org/10.1007/s11101-006-9040-2

    Article  CAS  Google Scholar 

  7. Damjanovich, S., Gáspár, R., and Panyi, G., An alternative to conventional immuno suppression: small-molecule inhibitors of Kv1. 3 channels, Mol. Int., 2004, vol. 4, pp. 250–254. https://doi.org/10.1124/mi.4.5.4

    Article  CAS  Google Scholar 

  8. De Wolf, H., Blust, R., and Backeljau, T., The use of RAPD in ecotoxicology, Mut. Res./Rev. Mut. Res., 2004, vol. 566, pp. 249–262. https://doi.org/10.1016/j.mrrev.2003.10.003

    Article  CAS  Google Scholar 

  9. Gnonlonfin, G.B., Sanni, A., and Brimer, L., Review scopoletin—a coumarin phytoalexin with medicinal properties, Crit. Rev. Plant Sci., 2012, vol. 31, pp. 47–56. https://doi.org/10.1080/07352689.2011.616039

    Article  CAS  Google Scholar 

  10. Kawase, M., Sakagam, H., Motohash, N., et al., Coumarin derivatives with tumor-specific cytotoxicity and multi drug resistance reversal activity in vivo, IV. IIAR J., 2005, vol. 19, pp. 705–711.

    CAS  Google Scholar 

  11. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685. https://doi.org/10.1038/227680a0

    Article  CAS  PubMed  Google Scholar 

  12. Liu, W., Yang, Y.S., Zhou, Q., et al., Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers, Chemosphere, 2007, vol. 67, pp. 1155–1163. https://doi.org/10.1016/j.chemosphere.2006.11.011

    Article  CAS  PubMed  Google Scholar 

  13. Malaiyandi, V., Sellers, E.M., and Tyndale, R.F., Implications of CYP2A6 genetic variation for smoking behaviors and nicotine dependence, Clin. Pharm. Ther., 2005, vol. 77, pp. 145–158. https://doi.org/10.1016/j.clpt.2004.10.011

    Article  CAS  Google Scholar 

  14. Nei, M., Genetic distance between populations, Am. Nat., 1972, vol. 106, pp. 283–292. https://doi.org/10.1086/282771

    Article  Google Scholar 

  15. Ozek, N., Bal, I., Sara, Y., et al., Structural and functional characterization of simvastatin-induced myotoxicity in different skeletal muscles, Biochim. Biophys. Acta, 2014, vol. 1840, pp. 406–415. https://doi.org/10.1016/j.bbagen.2013.09.010

    Article  CAS  Google Scholar 

  16. Plumas, J., Drillat, P., Jacob, M., et al., Extra corporeal photochemotherapy for treatment of clonal T cell proliferations, Bul. Ducan., 2003, vol. 90, pp. 763–770.

    Google Scholar 

  17. Shinde, R.G., Khan, A.A., and Barik, A., Coumarin derivatives with antioxidant and anticancer potential: a review, Int. J. Med. Appl. Sci., 2014, vol. 3, pp. 165–184.

  18. Turker, S., Dogan, F.T., and Severcan, F., The characterization and differentiation of higher plants by Fourier transform infrared spectroscopy, Appl. Spectrosc., 2007, vol. 61, pp. 300–308.

    Article  Google Scholar 

  19. Venugopala, K.N., Rashmi, V., and Odhav, B., Review on natural coumarin lead compounds for their pharmacological activity, BioMed. Res. Int., 2013.

  20. Williams, J.G., Kubelik, A.R., Livak, K.J., et al., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucleic Acids Res., 1990, vol. 18, pp. 6531–6535. https://doi.org/10.1093/nar/18.22.6531

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Wulff, H., Rauer, H., Düring, T., et al., Alkoxypsoralens, novelnon peptide blockers of Shaker-type K+ channels: synthesis and photo reactivity, J. Med. Chem., 1998, vol. 41, pp. 4542–4549. https://doi.org/10.1021/jm981032o

    Article  CAS  PubMed  Google Scholar 

  22. Xu, R.X., Gao, S., Zhao, Y., Lou, H.X., and Cheng, A.X., Functional characterization of a Mg2+-dependent O‑methyltransferase with coumarin as preferred substrate from the liverwort Plagiochasma appendiculatum, Plant Physiol. Biochem., 2016, vol. 106, pp. 269–277.

  23. Yuksel, B. and Aksoy, O., Cytological effects of coumann on the mitosis of Lens culinaris Medik, Fresemus Env. Bull., 2017, vol. 26, pp. 6400–6407.

    CAS  Google Scholar 

  24. Yuksel, B. and Aksoy, O., Analysis of the effects of coumarin on Lens culinaris Medik by some biochemical parameters using real-time polymerase chain reaction, Legume Res., 2019, vol. 42, pp. 640–645. https://doi.org/10.18805/LR-439

    Article  Google Scholar 

Download references

ACKNOWLEDGMENTS

We would like to express our gratitude to Kocaeli University [BAP-2016-02] for their financial support during this study.

Author information

Authors and Affiliations

  1. Kocaeli University, Umuttepe, Izmit, Kocaeli, 41380, Turkey

    Burcu Yuksel, Ozlem Aksoy & Togayhan Kutluk

Authors
  1. Burcu Yuksel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ozlem Aksoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Togayhan Kutluk
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Burcu Yuksel.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yuksel, B., Aksoy, O. & Kutluk, T. Molecular and Spectroscopic Evaluation of the Effects of Coumarin on Lentil. Cytol. Genet. 55, 274–282 (2021). https://doi.org/10.3103/S0095452721030117

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0095452721030117

Keywords:

Search

Navigation